ABSTRACT
Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embryonic mouse NSCs developed into morphologically and biochemically fully maturated neurons, with extensive dendrites and multiple synaptic contacts. However, even after 22 days of culture, none of these neurons developed voltage-dependent sodium-channels characteristic for a functional neuron. Apparently, the morphological differentiation and the electrophysiological maturation of an embryonic mouse NSC into a neuron are independently regulated.
Subject(s)
Cell Differentiation/physiology , Neurons/cytology , Stem Cells/cytology , Animals , Cells, Cultured , Electric Stimulation/methods , Embryo, Mammalian , Epidermal Growth Factor/physiology , Fibroblast Growth Factor 2/physiology , Glial Fibrillary Acidic Protein/physiology , Immunohistochemistry/methods , Membrane Potentials/physiology , Membrane Potentials/radiation effects , Mice , Mice, Inbred C57BL , Microtubule-Associated Proteins/metabolism , Neurons/physiology , Patch-Clamp Techniques/methods , Stem Cells/physiologyABSTRACT
A unique insight into characteristics, motivation, and behavior of problem complete denture patients is colorfully presented.
Subject(s)
Consumer Behavior , Denture, Complete , Wit and Humor as Topic , Bruxism/physiopathology , Communication , Dentist-Patient Relations , Female , Gagging , Humans , Male , PersonalityABSTRACT
The problems involved in providing comfort, function, proper esthetics, and retention for the maxillary complete denture patient with natural opposing dentition can be challenging. Careful preparation of the patient is important. It provides the new complete denture patient the opportunity to adapt to a complete denture and allows the dentist to evaluate his patient physically and emotionally before fabrication of the final complete denture. Methods for planning and fabrication of a denture that provide comfort, function, and stability have been described.
Subject(s)
Dentition , Denture, Complete, Upper , Adaptation, Physiological , Consumer Behavior , Dental Occlusion, Centric , Denture Design , Denture Retention , HumansABSTRACT
Using an albumen-filled column, it was possible to separate highly motile sperm from autoagglutinated, dead and immotile sperm, as well as from other seminal debris. Motility was increased from a mean of 45.4 to 74.3% (P less than 0.001), although the sperm density fell from a mean of 28.2 million per ml to 9.7 million per ml (P less than 0.001). When these selected highly concentional AlH had been repeatedly tried without success, 3 pregnancies were achieved in 18 women (16.7%).
