Short and long latency response due to transition from bilateral to unilateral contraction.

The aim of this study was to investigate the effect of fast and slow relaxation of the knee extensor muscle of the dominant leg on torque-time curve of the unilaterally contracting contralateral muscle. Eight adult male subjects were recruited without bilateral deficit. In Task 1 subjects exerted bilateral and unilateral torque and bilateral index was calculated. In Task 2 subjects relaxed the right knee extensors as slow as possible while maximum activation of the contralateral muscle had to be maintained. In Task 3 the relaxation was as fast as possible. During slow relaxation the short latency response (dM1) was 6.6% torque reduction in the left leg. On the contrary fast muscle relaxation resulted in a 7.3% increase. During long latency response the torque increased in both tasks, but did not exceed the torque measured in Task 1. Significant correlation was found between the rate of torque reduction (RTR) and dM1 (r = 0.95, P < 0.001), time to peak (t1) and dM1 (r = 0.812, P < 0.01). The regression analysis indicated that RTR greater or less than -1.0 Nm/ms results in opposite short latency response. We concluded that the different tasks for two knee extensor muscles result in transient interhemispheric effects which are time and rate of torque reduction dependent.

[Secondary malignancies in urinary diversions]. / Sekundärmalignome in Harnableitungen.

In contrast to ureterosigmoidostomy no reliable clinical data exist for tumor risk in different forms of urinary diversion using isolated intestinal segments.In 44 German urological departments, operation frequencies, indications, patient age, and operation dates of the different forms of urinary diversion, operated between 1970 and 2007, could be registered. The secondary tumors up to 2009 were registered as well and related to the numbers of the different forms of urinary diversions resulting in tumor prevalences.In 17,758 urinary diversions 32 secondary tumors occurred. The tumor risk in ureterosigmoidostomy (22-fold) and cystoplasty (13-fold) is significantly higher than in other continent forms of urinary diversion such as neobladders or pouches (p<0.0001). The difference between ureterosigmoidostomy and cystoplasty is not significant, nor is the difference between ileocecal pouches (0.14%) and ileal neobladders (0.05%) (p=0.46). The tumor risk in ileocecal (1.26%) and colonic neobladders (1.43%) is significantly higher (p=0.0001) than in ileal neobladders (0.5%). Of the 16 tumors that occurred following ureterosigmoidostomy, 16 (94%) developed directly at the ureterocolonic borderline in contrast to only 50% following urinary diversions via isolated intestinal segments.From postoperative year 5 regular endoscopic controls of ureterosigmoidostomies, cystoplasties, and orthotopic (ileo-)colonic neobladders are necessary. In ileocecal pouches, regular endoscopy is necessary at least in the presence of symptoms or should be performed routinely at greater intervals. Following neobladders or conduits, only urethroscopies for urethral recurrence are necessary.

Cistectomía radical laparoscópica asistida por robot: evaluación de los resultados funcionales y oncológicos / Robotic-Assisted Laparoscopic Radical Cystectomy: Evaluation of Functional and Oncological Results

Objetivo: presentamos nuestra experiencia en cistectomía radical robótica informando sobre los resultados quirúrgicos, tanto desde el punto de vista oncológico como funcional. Material y método: entre enero de 2007 y agosto de 2010 fueron operados en nuestra institución un total de 84 pacientes (70 hombres y 14 mujeres) con diagnóstico histopatológico de cáncer de vejiga invasor no metastásico. Evaluamos variables quirúrgicas, el tiempo de recuperación intrahospitalario, los hallazgos patológicos y las complicaciones peri y postoperatorias. Resultados: la edad media de la población en estudio fue 65,6 años (rango: 28-82). En 62 pacientes se realizó derivación urinaria con ureteroileostomía tipo Wallace, los 22 pacientes restantes recibieron una neovejiga ileal tipo Studer. El tiempo promedio de cirugía fue de 261 minutos (rango: 243-618) y la pérdida promedio de sangre intraoperatoria fue de 298ml (rango 50-2.000). Un 29, 38, 26 y 7% presentaron estadios histopatológicos postoperatorios pT1, pT2, pT3 y pT4, respectivamente. El 15% de los pacientes operados presentó metástasis locales a nivel ganglionar. El número de ganglios linfáticos resecados por procedimiento quirúrgico fue de 14,47 (rango: 1-33). En dos casos (2,4%) se registraron márgenes quirúrgicos positivos. El tiempo promedio de aparición de flatos fue 2,12 días, evacuación intestinal postoperatoria 2,87 y alta médica 17,710-33. Un total de 45 pacientes presentaron complicaciones perioperatorias. De estos sólo un 11,9% tuvo complicaciones mayores (Clavien III o mayor) que necesitaron de un tratamiento más invasivo. El tiempo promedio de seguimiento fue de 16,7 meses. Durante ese período 10 pacientes (11%) presentaron recidiva del tumor vesical, de los cuales dos (2,38%) fallecieron. Conclusión: nuestra experiencia con cistectomía radical robótica en el tratamiento del cáncer de vejiga sugiere que en manos adecuadas este procedimiento proporciona resultados quirúrgicos y patológicos aceptables (AU)

Purpose: radical cystectomy remains the most effective treatment for patients with localized, invasive bladder cancer and recurrent noninvasive disease. We report our experience with 84 consecutive cases of robotic assisted laparoscopic radical cystectomy with regard to perioperative results, pathological outcomes and surgical complications. Materials and methods: a total of 84 consecutive patients (70 male and 14 female) underwent robotic radical cystectomy and urinary diversion at our institution from January 2007 to August 2010 for clinically localized bladder cancer. Outcome measures evaluated included operative variables, hospital recovery, pathological outcomes and complication rate. Results: mean age of this cohort was 65.5 years (range 28 to 82). Of the patients 62 underwent ileal conduit diversion, 22 received a neobladder. Mean operating room time for all patients was 261min. (range: 243-618min.) and mean surgical blood loss was 298ml (range: 50-2000ml). 29% of the cases were pT1 or less disease, 38% were pT2, 26% and 7% were pT3 and T4 disease respectively, 15% were node positive. Mean number of lymph nodes removed was 15 (range 1 to 33). In 2 cases (2.4%) there was a positive surgical margin. Mean days to flatus were 2.12, bowel movement 2.87 and discharge home 17.7 (range: 10-33). There were 45 postoperative complications with 11.9% having a major complication (Clavien grade 3 or higher). At a mean followup of 16.7 months 10 patients (11%) had disease recurrence and 2 died of disease. Conclusions: our experience with robotic radical cystectomy for the treatment of bladder cancer suggests that in proper hands this procedure provides acceptable surgical and pathological outcomes (AU)

[Robotic-assisted laparoscopic radical cystectomy: evaluation of functional and oncological results]. / Cistectomía radical laparoscópica asistida por robot: evaluación de los resultados funcionales y oncológicos.

PURPOSE: radical cystectomy remains the most effective treatment for patients with localized, invasive bladder cancer and recurrent noninvasive disease. We report our experience with 84 consecutive cases of robotic assisted laparoscopic radical cystectomy with regard to perioperative results, pathological outcomes and surgical complications. MATERIALS AND METHODS: a total of 84 consecutive patients (70 male and 14 female) underwent robotic radical cystectomy and urinary diversion at our institution from January 2007 to August 2010 for clinically localized bladder cancer. Outcome measures evaluated included operative variables, hospital recovery, pathological outcomes and complication rate. RESULTS: mean age of this cohort was 65.5 years (range 28 to 82). Of the patients 62 underwent ileal conduit diversion, 22 received a neobladder. Mean operating room time for all patients was 261min. (range: 243-618min.) and mean surgical blood loss was 298ml (range: 50-2000ml). 29% of the cases were pT1 or less disease, 38% were pT2, 26% and 7% were pT3 and T4 disease respectively, 15% were node positive. Mean number of lymph nodes removed was 15 (range 1 to 33). In 2 cases (2.4%) there was a positive surgical margin. Mean days to flatus were 2.12, bowel movement 2.87 and discharge home 17.7 (range: 10-33). There were 45 postoperative complications with 11.9% having a major complication (Clavien grade 3 or higher). At a mean followup of 16.7 months 10 patients (11%) had disease recurrence and 2 died of disease. CONCLUSIONS: our experience with robotic radical cystectomy for the treatment of bladder cancer suggests that in proper hands this procedure provides acceptable surgical and pathological outcomes.

Rapid analysis of abietanes in conifers.

Diterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis of abietanes by reversed-phase high-performance liquid chromatography using multiwavelength detection to optimize quantification of underivatized abietic, neoabietic, palustric, levopimaric, and dehydroabietic acids. The utility of the method is demonstrated with methanol extracts of white spruce (Picea glauca) phloem, and representative concentrations are reported.

Factor structure and psychometric properties of the brief Mizes Anorectic Cognitions questionnaire.

The Mizes Anorectic Cognitions-Revised (MAC-R) questionnaire is a 24-item self-report instrument designed to assess cognitions associated with anorexia and bulimia nervosa. We examined the factor structure and psychometric properties of the MAC-R in a sample of 290 undergraduates. We found that a brief 3-factor oblique model (composed of 12 items) of the MAC-R provided acceptable fit to the present sample data. This model was largely invariant across male and female participants. The brief version of the MAC-R (the BMAC) and subscale scores correlated significantly with scores from related measures of eating disorders, showing evidence of concurrent validity. The BMAC total and subscales also showed evidence of discriminant validity by differentiating between empirically defined subclinical eating disorders and control participants. Both logistic regression and discriminant function analyses showed that only the self-control and self-esteem subscale was most useful in distinguishing between the subgroups.

Development and psychometric properties of the Student Worry Questionnaire-30.

Described are the development and initial psychometric properties (Ns = 50 and 188) of a self-report measure, the Student Worry Questionnaire-30, for use with college undergraduates. Exploratory principal components analyses (Ns = 388, 350, and 396) with oblimin rotation indicated six domains of worrisome thinking, financial-related concerns, significant others' well-being, social adequacy concerns, academic concerns, and general anxiety symptoms. The total score and scale scores showed internal consistency of .80 to .94. Also, test-retest reliability analyses (.75 to .80) support consistency of responses over 4 wk. Strong evidence for convergent validity) was indicated. Confirmatory factor analysis confirmed the fit of the 6-factor oblique model. Limitations of the present studies, and directions for research are discussed.

Assessment of suicidal ideation in young men and women: the incremental validity of the MMPI-2 content scales.

This study of 139 women and 75 men investigated the incremental validity of the MMPI-2 in assessing suicidal ideation by examining the unique contribution of the 15 content scales beyond what is provided by the 13 basic validity and clinical scales. A multivariate analysis of variance resulted in a significant difference on the validity, clinical, and content scales for young men and women. Women scored significantly higher than men on the Hypochondriasis, Depression, Conversion Hysteria, and Masculinity-Femininity scales and lower than men on the Hypomania, Fears, Anger, and Type A scales. The results of the hierarchical regression analyses indicated that for women, hopelessness and reasons for living; the Correction, Paranoia, Conversion Hysteria, Psychopathic Deviate, and Hypomania scales; and the Anger content scale contributed significantly to the prediction of suicidal ideation. For men, hopelessness, the Lie and Hypomania scales, and the Type A content scale contributed significantly to the prediction of suicidal ideation. Thus, it would appear that a different pattern of concerns and difficulties are manifested in young men and women related to suicidal ideation. Limitations of the present study and future directions for research are discussed.

The Suicidal Behaviors Questionnaire-Revised (SBQ-R): validation with clinical and nonclinical samples.

Past suicidal behaviors including ideation and attempts have been identified as significant risk factors for subsequent suicidal behavior. However, inadequate attention has been given to the development or validation of measures of past suicidal behavior. The present study examined the reliability and validity of a brief self-report measure of past suicidal behavior, the Suicidal Behaviors Questionnaire-Revised (SBQ-R). Participants included psychiatric inpatient adolescents, high school students, psychiatric inpatient adults, and undergraduates. Logistic regression analyses provided empirical support for the usefulness of the SBQ-R as a risk measure of suicide to differentiate between suicide-risk and nonsuicidal study participants. Receiver operating characteristic (ROC) analyses indicated that the most useful cutoff scores on the SBQ-R were 7 for nonsuicidal samples, and 8 for clinical samples. Both the single SBQ-R Item 1 and SBQ-R total scores are recommended for use in clinical and nonclinical settings.

Development and initial validation of the Self-harm Behavior Questionnaire.

Evaluation of suicide-related behaviors and thoughts about suicide with a newly developed self-report instrument is described. The Self-Harm Behavior Questionnaire (SHBQ; Gutierrez, 1998) generates detailed clinical information from an easy to administer and score self-report form. A sample of 342 participants were drawn from university undergraduate students enrolled in various psychology courses. Participants were then divided into a severe suicidal ideation group (n = 20) and a nonsuicidal control group (n = 20) to conduct subgroup and criterion-related validity analyses. Preliminary analyses indicate this new questionnaire is both valid and reliable when used with young adults drawn from a nonclinical population. The questionnaire should be of use to both clinicians and researchers due to the flexibility of the data generated.

Consequences of elevated carbon dioxide and ozone for foliar chemical composition and dynamics in trembling aspen (Populus tremuloides) and paper birch (Betula papyrifera).

Atmospheric chemical composition affects foliar chemical composition, which in turn influences the dynamics of both herbivory and decomposition in ecosystems. We assessed the independent and interactive effects of CO2 and O3 fumigation on foliar chemistry of quaking aspen (Populus tremuloides) and paper birch (Betula papyrifera) at a Free-Air CO2 Enrichment (FACE) facility in northern Wisconsin. Leaf samples were collected at five time periods during a single growing season, and analyzed for nitrogen. starch and condensed tannin concentrations, nitrogen resorption efficiencies (NREs), and C:N ratios. Enriched CO2 reduced foliar nitrogen concentrations in aspen and birch; O3 only marginally reduced nitrogen concentrations. NREs were unaffected by pollution treatment in aspen, declined with 03 exposure in birch, and this decline was ameliorated by enriched CO2. C:N ratios of abscised leaves increased in response to enriched CO2 in both tree species. O3 did not significantly alter C:N ratios in aspen, although values tended to be higher in + CO2 + O3 leaves. For birch, O3 decreased C:N ratios under ambient CO2 and increased C:N ratios under elevated CO2. Thus, under the combined pollutants, the C:N ratios of both aspen and birch leaves were elevated above the averaged responses to the individual and independent trace gas treatments. Starch concentrations were largely unresponsive to CO2 and O3 treatments in aspen. but increased in response to elevated CO2 in birch. Levels of condensed tannins were negligibly affected by CO2 and O3 treatments in aspen, but increased in response to enriched CO2 in birch. Results from this work suggest that changes in foliar chemical composition elicited by enriched CO2 are likely to impact herbivory and decomposition, whereas the effects of O3 are likely to be minor, except in cases where they influence plant response to CO2.

The Pain Catastrophizing Scale: further psychometric evaluation with adult samples.

Previous studies with undergraduates have provided support for the reliability and oblique three-factor structure of a new scale, the Pain Catastrophizing Scale (PCS). We examined the reliability and validity of the PCS in adult community and pain outpatient samples. The PCS showed a high internal consistency in both groups. Using data from the community sample, confirmatory factor analyses showed that the PCS taps a single construct characterized by three related dimensions. Gender differences were obtained on the PCS total score in the community and the outpatient samples. The analyses also showed significant differences between the community and the outpatient samples on the PCS total and subscales. Overall, the results showed strong evidence of criterion-related, concurrent, and discriminant validity for the PCS in the community sample. Limitations of the present study are discussed.

Why young people do not kill themselves: the reasons for living inventory for adolescents.

Assessed the reliability, validity, and predictive power of a new measure, the Reasons for Living Inventory for Adolescents (RFL-A; Osman et al., 1998). A group of 206 (101 boys and 105 girls) adolescent psychiatric inpatients completed the RFL-A, Minnesota Multiphasic Personality Inventory for Adolescents (Butcher et al., 1992), and a packet of self-report measures. Additional information about the patients including diagnosis and suicide status were obtained from their medical records. It was determined that the RFL-A is a valid and reliable measure of adolescent suicide risk potential. Additionally, the RFL-A possesses better predictive power than the Beck Hopelessness Scale (Beck, Weissman, Lester, & Trexler, 1974). A discussion of the clinical and research utility of the RFL-A is included along with suggestions for future research.

The multi-attitude suicide tendency scale: further validation with adolescent psychiatric inpatients.

The present study was designed to examine the construct validity and psychometric properties of the Multi-Attitude Suicide Tendency Scale in adolescent psychiatric inpatients. This new scale has four subscales: attraction to life, repulsion by life, attraction to death, and repulsion by death. Confirmatory factor analysis of the scale items provided support for the fit of the 28-item, four-factor model. Results of logistic regression analyses revealed that high scores on the repulsion by life and the attraction to death subscales were useful in assessing the risk for suicide attempts. Results of the hierarchical regression analysis showed that high repulsion by life and low attraction to life subscale scores contributed to the prediction of scores on the suicide probability scale. We also examined gender differences on the four subscales and the psychological correlates of each subscale.

Functional analysis of different LMP1 proteins isolated from Epstein-Barr virus-positive carriers.

The Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis and is implicated in the development of several human malignancies. Latent membrane protein 1 (LMP1), an EBV protein with known oncogenic properties, may be important in the pathogenesis of EBV-associated tumors, particularly nasopharyngeal carcinoma (NPC) and Hodgkin's disease (HD). Several reports suggested that sequence variations in the LMP1 gene may define a more aggressive, geographically restricted EBV-genotype. Most mutations in the LMP1 gene described are located within the C-terminus of the protein. However, the effect of these mutations on the biological function of the protein remains widely unknown. Therefore, this study aimed in investigating whether mutations detected in LMP1 genes isolated from different EBV-positive carriers have an effect on the biological function of the protein. For this purpose the LMP1 genes were amplified by nested PCR from DNA out of bone marrow and peripheral blood lymphocytes and sequenced. Three functional assays were performed in order to evaluate the biological activity of the different isolates: activation of the transcription factors NF-kappaB and AP-1 as well as the anchorage independent growth of LMP1 transfected ratl cells in soft agar. The results suggested that whereas differences in the activation of NF-kappaB through the various LMP1 isolates correlated tightly with their different expression levels, the outgrowth of transfected cells in soft agar did not and the transcription factor NF-kappaB therefore appeared not to be the major effector for the transformation of the rodent cell line ratl by LMP1. The various LMP1-isolates also differed in their capacity in activating the transcription factor AP-1. We found no correlation between the transforming ability of the LMPI isolates and activation of AP-1 suggesting that other so far uncharacterized domains also influence the transforming ability of the protein.

The Reasons for Living Inventory for Adolescents (RFL-A): development and psychometric properties.

The role of adaptive beliefs and attitudes against suicide has not been given adequate attention in the clinical or assessment literature. This article reports on the development and initial psychometric properties of a 32-item self-report inventory, the Reasons for Living Inventory for Adolescents (RFL-A). In Phase 1, we used exploratory and confirmatory factor analyses to identify five correlated factors: Future Optimism, Suicide-Related Concerns, Family Alliance, Peer Acceptance and Support, and Self-Acceptance. In Phase 2, we cross-validated the 5-factor oblique model in a different group of adolescents recruited from two high schools. In addition, we examined evidence for convergent, discriminant, and construct validities. The coefficient alpha indices for the RFL-A total and scales were satisfactory. In Phase 3, we evaluated additional evidence of reliability and validity using samples of high school and psychiatric inpatient adolescents. The results suggest that the RFL-A is a short, reliable, and valid measure that is potentially useful in the assessment of adolescent suicidal behavior.

The positive and negative suicide ideation inventory: development and validation.

We conducted two studies to develop and validate a brief self-report measure for assessing the frequency of positive and negative thoughts related to suicidal behavior. Items on this new measure, the Positive and Negative Suicide Ideation inventory, were generated by undergraduates. In Study 1, we administered a 20-item version of the inventory to 150 male and 300 female undergraduates and conducted an exploratory principal axis factor analysis with varimax rotation. Two factors, Positive Ideation and Negative Ideation, were retained. In Study 2, we conducted a confirmatory factor analysis to validate the fit of the one-factor and the oblique two-factor models to data from another sample of 84 men and 202 women. The oblique two-factor model provided an excellent fit to the sample data. We also examined preliminary evidence of concurrent and predictive validity. Over-all, these findings suggested that the inventory is a well-developed self-report measure for assessing the frequency of positive and negative thoughts related to suicidal behavior.

Clinical utility of the MMPI-A content scales and Harris-Lingoes subscales in the assessment of suicidal risk factors in psychiatric adolescents.

This study of 143 inpatient adolescents (68 boys and 75 girls) investigated the clinical utility of the MMPI-A in assessing suicidal risk factors by examining the unique contribution of the content scales and Harris-Lingoes subscales beyond what is provided by the basic clinical scales. The results of the regression analyses indicated that for boys, the Depression, Psychopathic Deviate and Hypomania scales; Alienation and Anxiety content scales: and Subjective Depression. Self Alienation, Imperturbability, and Amorality Harris-Lingoes subscales contributed significantly to the prediction of suicide probability. For girls, the Depression, Psychopathic Deviate, and Hypomania scales; Family Problems, Conduct Problems, School Problems, Depression, and Social Discomfort content scales; and the Subjective Depression, Self Alienation, Psychomotor Acceleration, and Imperturbability Harris-Lingoes subscales contributed significantly to the prediction of suicide probability.

The Beck Anxiety Inventory: reexamination of factor structure and psychometric properties.

Several exploratory factor-analytic studies of the Beck Anxiety Inventory (BAI; Beck, Epstein, Brown, & Steer, 1988) have reported two, four, and five factors. This study evaluated the fit of four competing models to data provided by a sample of 350 undergraduates. Results of the initial confirmatory factor analyses (CFA) provided strong support for the fit of the four-factor oblique model. Next, we respecified the four-factor model as a single second-order BAI. Results showed that the second-order model also provided adequate fit to the data. Evidence also supported the psychometric indices of reliability and convergent validity. Finally, we examined the relation of the BAI to several demographic variables. Limitations of the study are discussed.