ABSTRACT
The essence of studies was that the disease is simulated in 12-day albino mice subcutaneously infected with Hantaan virus, strain 76-118 in a dose of 10 LD50. As an efficiency index, the study of drugs uses major (death protection coefficient, mean animal lifetime) and auxiliary (virological: pathogen accumulation in the brain tissues of deceased animals) parameters, biochemical (the levels of creatinine, urea, alanine aminotransferase, aspartate aminotransferase, malonic dialdehyde), hematological (count of leukocytes, leukogram) ones; as well as interferon status (the levels of circulatory interferon, leukocytic production of alpha- and gamma-interferons). The procedure for simulating the disease caused by Hantaan virus on an experimental animal is used to choose effective drugs against the pathogen of hemorrhagic nephrosonephritis.
Subject(s)
Disease Models, Animal , Hantaan virus , Hemorrhagic Fever with Renal Syndrome , Mice , Alanine Transaminase/blood , Animals , Animals, Newborn , Antiviral Agents/isolation & purification , Antiviral Agents/pharmacology , Aspartate Aminotransferases/blood , Brain/virology , Creatinine/blood , Disease Susceptibility , Drug Evaluation, Preclinical , Guinea Pigs , Hantaan virus/drug effects , Hemorrhagic Fever with Renal Syndrome/blood , Hemorrhagic Fever with Renal Syndrome/immunology , Hemorrhagic Fever with Renal Syndrome/virology , Interferons/biosynthesis , Lethal Dose 50 , Leukocyte Count , Lymphocyte Activation , Lymphocytes/immunology , Malonates/blood , Rabbits , Rats , Urea/bloodABSTRACT
The antiviral effectiveness of the combined and single use of superlow-dose amixine and virasole on the course of experimental hemorrhagic fever with renal syndrome was studied in sucking albino mice parenterally infected with their virus Hantaan. The co-administration of virasole and amixine was shown to protect 52% of the infected animals from death, which is superior to the effect of their monotherapy. The combined use of the drugs substantially prolongs the survival of albino mice after their infection and the level of brain viral reproduction suppression ( delta = 3.21 g) in the experimental group as compared to the controls and to the mice given only one of the drugs.
Subject(s)
Antiviral Agents/therapeutic use , Hantaan virus , Hemorrhagic Fever with Renal Syndrome/drug therapy , Ribavirin/therapeutic use , Tilorone/therapeutic use , Animals , Animals, Suckling , Antiviral Agents/administration & dosage , Brain/virology , Drug Administration Schedule , Drug Evaluation, Preclinical , Drug Therapy, Combination , Hemorrhagic Fever with Renal Syndrome/virology , Ribavirin/administration & dosage , Tilorone/administration & dosageABSTRACT
Efficacy of drug therapy of Omsk hemorrhagic fever (OHF) was tested experimentally using cell culture and laboratory animals infected with the OHF virus strain Ondatra. Screening tests showed that high concentrations of Virazol or interferon inducers Larifan and Rifastin caused moderately pronounced suppression of virus reproduction in cell culture. Realdiron was found to be a high-efficacy preparation causing complete inhibition of virus reproduction in cell. Larifan demonstrated the highest antiviral efficacy against OHF virus in experiments with laboratory animals. This drug prevented the death of 65% infected mice and significantly decreased infection process severity in rabbits.
