ABSTRACT
Age-dependent angiogenesis intensity changes have been studied in transgenic HER-2/neu (FBV/N) mice characteristic of breast tumors' high incidence with hyperexpression of HER-2/neu. Concentration of vascular endothelial growth factor, insulin-dependent growth factor 1, nitrogen monoxide, tissue plasminogen activator and type 1 plasminogen activator inhibitor were assessed by means of immune-enzyme assay. The results testify to angiogenesis processes activation side by side with aging and growth of the tumors. Maximum manifestation of these disturbances (growth factors' blood concentrations increase and endotheliocytes' functional activity inhibition) has been revealed in 6-month-old mice during neoplasma maximum intensive and aggressive growth period.
Subject(s)
Adenocarcinoma/blood , Aging/blood , Biomarkers, Tumor/blood , Mammary Neoplasms, Animal/blood , Neovascularization, Pathologic/blood , Receptor, ErbB-2/blood , Adenocarcinoma/blood supply , Aging/metabolism , Aging/pathology , Animals , Female , Insulin-Like Growth Factor I/metabolism , Mammary Neoplasms, Animal/blood supply , Mammary Neoplasms, Experimental , Mice , Mice, Inbred Strains , Mice, Transgenic , Nitric Oxide/blood , Plasminogen Activator Inhibitor 1/blood , Tissue Plasminogen Activator/blood , Vascular Endothelial Growth Factor A/bloodABSTRACT
Blood levels of vascular endothelial growth factor, the main marker of angiogenesis activity, and coagulation hemostasis were studied in CDF1 mice with transplanted P-388 lymphocytic leukemia. Blood levels of vascular endothelial growth factor increased by 168% in mice with tumors. The animals developed the hypercoagulation syndrome manifesting in shorter activated partial thromboplastin time and prothrombin time and development of hyperfibrinogenemia.
