1.
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
Nature
; 317(6034): 230-4, 1985.
Article
in English
| MEDLINE
| ID: mdl-2995814
ABSTRACT
A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
Subject(s)
DNA Transposable Elements , DNA, Recombinant/metabolism , Genes , Globins/genetics , Base Sequence , Chromosome Mapping , Cloning, Molecular , DNA Restriction Enzymes , Humans , Nucleic Acid Hybridization
2.
Cold Spring Harb Symp Quant Biol
; 49: 161-70, 1984.
Article
in English
| MEDLINE
| ID: mdl-6597754
