Retinal projections to the accessory optic system in pigmented and albino ferrets (Mustela putorius furo).

We investigated if a reduced specificity of the retinal projection to the accessory optic system might be responsible for the loss of direction selectivity in the nucleus of the optic tract and dorsal terminal nucleus (NOT-DTN) and, in consequence of this, the optokinetic deficits in albino ferrets. Under electrophysiological control we performed dual tracer injections into the NOT-DTN and the medial terminal nucleus (MTN). Retrogradely labelled ganglion cells were found in the visual streak, the dorsal, and the ventral retina both after injections into the NOTDTN and the MTN indicating that both nuclei receive input from the same retinal regions. The distribution and spacing of labelled ganglion cells did not differ between pigmented and albino ferrets. However, retinal ganglion cells projecting simultaneously to both the NOT-DTN and the MTN occurred only in albino ferrets. These results suggest that a reduced specificity of the projection pattern of direction specific ganglion cells may contribute to the loss of direction selectivity in the NOT-DTN in albino ferrets.

Long-term evaluation of orofacial function in children with Down syndrome after treatment with a stimulating plate according to Castillo Morales.

The aim of this investigation was to evaluate the long-term orofacial development of Down children who received plate therapy according to Castillo Morales in their early childhood. The orofacial development of 27 Down children was documented before and after plate therapy and at a follow-up examination 13 years +/- 6 months after initiation of therapy. The orofacial appearance significantly improved during therapy (p = 0.00). During the follow-up, mouth posture remained stable (p = 0.259), whereas tongue position further improved (p = 0.034). A better long-term development was documented in children with initial severe orofacial dysfunctions.

Evaluation of an antimicrobial and fluoride-releasing self-etching primer on the shear bond strength of orthodontic brackets.

The enhanced risk of dental caries is one negative side-effect of fixed appliances. A new antimicrobial and fluoride-releasing self-etching bonding system (Clearfil Protect Bond) has been introduced in restorative dentistry and clinical studies have already shown the potential for this primer to be used clinically with effective antiplaque properties. Therefore, this in vitro study was conducted in order to evaluate the new primer in comparison with a conventional bonding preparation. One hundred and twenty extracted human teeth were randomly divided into three groups of 40 specimens each (20 incisors, 20 premolars). In group 1 a conventional bonding procedure was used (etching, Transbond XT), in group 2 the new primer was used according to the manufacturer's recommendations when bonding to intact enamel, while in group 3 the new primer was used without prior etching. Shear bond strength (SBS) was measured with a universal testing machine and the adhesive remaining after debonding was determined using an optical microscope at x10 magnification. The adhesive remnant index was used in order to assess the mode of failure. No enamel fractures were detected in any of the specimens. In all groups acceptable bond strengths were observed. The only statistically significant difference (P = 0.004) was found for the incisors in group 2, which showed the highest mean SBS (17.46 MPa). Considering the acceptable bond strength and the mode of failure, use of the new primer without prior etching is recommended in patients with fixed appliances. Further in vivo studies will be carried out in order to evaluate clinical performance.

Dentomaxillofacial imaging in Proteus syndrome.

Proteus syndrome is a rare condition that involves atypical growth of the bones, skin and head and a variety of other symptoms. Only a few authors have reported on the craniofacial manifestations so far. The authors present a case of a 7-year-old girl with Proteus syndrome in which the facial skeleton showed unilateral overgrowth. The analysis of the radiological evaluation revealed a bialveolar prognathism, a skeletal class III, a dolicocephalic growth pattern and a left convex face scoliosis. On the left side, the lesser wing of the sphenoid was elevated and the ethmoidal cell complex was hypertrophic. The left ramus and body of the mandible were enlarged. The asymmetric dental development with a precocious dental age on the affected side was the most striking feature on the panoramic view. Early diagnosis and therapy depend on clinical evaluation and imaging. Therefore, further evaluations on the craniofacial features of patients with Proteus syndrome are necessary in order to establish a list of characteristic symptoms.

Contribution of inhibitory mechanisms to direction selectivity and response normalization in macaque middle temporal area.

Inhibitory mechanisms contribute to directional tuning in primary visual cortex, and it has been suggested that, in the primate brain, the middle temporal area (MT) inherits most of its directional information from primary visual cortex (V1). To test the validity of this hierarchical scheme, we investigated whether directional tuning in MT was present upon blockade of local gamma-aminobutyratergic (GABAergic) inhibitory mechanisms. Direction selectivity during the initial 50 ms after response onset was abolished in many MT cells when the local inhibitory network was inactivated whereas direction selectivity in later response periods was largely unaffected. Thus, direction selectivity during early response periods is often generated autonomously within MT whereas direction selectivity during later response periods is either inherited from other visual areas or locally mediated by mechanisms other than gamma-aminobutyric acid type A receptor (GABA(A)) inhibition. GABAergic inhibition may also mediate contrast normalization. Our data suggest that GABA(A) inhibition implements a local direction-selective static nonlinearity, rather than a full normalization in MT. These findings put constraints on strict hierarchical models according to which MT performs more complex computations based on local motion measurements provided by earlier areas, arguing for more distributed and independent information processing.

Optimizing interdisciplinary cooperation for patients with orofacial dysfunctions. Presentation of an interdisciplinary diagnostic referral sheet.

BACKGROUND: The quality of interdisciplinary cooperation is one of the most important factors in the success and long-term stability of treatment of patients suffering from orofacial dysfunctions. However, speech pathologists have criticized the poor communication and diagnostics at the time of referral and have requested a standardized diagnostic referral sheet with the aim of improving interdisciplinary cooperation. DIAGNOSTIC REFERRAL SHEET: The diagnostic referral sheet presented here is based on the demands of speech pathologists focusing on myofunctional therapy and helps in initial assessment of the individual situation and necessary treatment. It is subject to continuous updating and coordinates the many medical disciplines involved on account of the complex pathology. CONCLUSION: The multidisciplinary diagnostic referral sheet, which is independent of treatment methods and compressed into one page, is aimed at contributing to quality assurance and to the improved documentation of orofacial dysfunctions.

Early orthodontic treatment of Class-III malocclusion in Germany.

In order to evaluate current attitudes to early interceptive treatment, 2001 orthodontic offices in Germany were asked to fill in a questionnaire comprising the following topics: indication, appliances for the early correction of Class-III malocclusions, diagnostic records, duration, and benefits to overall therapy. Based on the 677 evaluable questionnaires, the following statistically significant conclusions could be drawn: 92.6% of the orthodontists see Class-III malocclusion as an indication for early treatment. Early treatment of severe crowding, diastemata, Class-II malocclusion, deep bite, increased overjet and impacted incisors was declined by most orthodontists. The interceptive treatment of further malocclusions was controversially discussed. Functional appliances (67.5%), in particular the Fränkel III (47.3%), were dominant in correction of Class-III malocclusions. Typical orthodontic records relating to early interceptive treatment include panoramic radiographs, lateral headfilms, photos and dental casts. 2.5% of the orthodontists routinely take a hand-wrist radiograph. Although recently published studies support the use of facial masks in theory, they are rarely used in practice. To what extent early interceptive treatment of Class-III malocclusion influences the overall treatment is the subject of further studies.

Influence of orthodontic appliances on myofunctional therapy.

Various removable and fixed orthodontic appliances were rated by interview and questionnaire by 42 myofunctional therapists in the Hamburg area with respect to their influence during myofunctional therapy. The Nance holding arch was given the most negative rating of all appliances covering the palate area. For the active plate, marking the rest position of the tongue by roughening the acrylic surface or by reproducing the palatal relief was considered beneficial. The quadhelix expansion device and Hyrax palatal expander were rated as unfavorable because of their positioning in the palatal area. Among the functional appliances, Fränkel's function regulator was given the best rating. Regular fixed appliances (brackets, bands) were not considered a disturbance. Habit reminders (plates and spurs) were given a very negative rating by ca. 80% of the therapists because they disturbed the myofunctional exercises and led to adaptive dysfunctions. Since many patients with dysfunction of the orofacial musculature undergo simultaneous myofunctional and orthodontic therapy, treatment planning and choice of orthodontic appliances should be carefully coordinated.

Development of the optokinetic system in macaque monkeys.

Optokinetic nystagmus in response to horizontal movement of a whole field random dot pattern was measured in infant macaque monkeys from the first week to about 5 months after birth using electrooculography. During monocular and binocular viewing conditions stimulus velocities were varied between 10 and 120 degrees/s. Monocular stimulation in the temporonasal direction yielded slow phase gain of the optokinetic system which was relatively constant for a given stimulus velocity over the whole period of observation. Gain during nasotemporal stimulation was also clearly present but significantly lower at early stages and increased during further development. This asymmetry of monocular horizontal optokinetic nystagmus (OKN) clearly depended on the stimulus velocity. At lower stimulus velocities (10-20 degrees/s) OKN was largely symmetrical at 2-5 weeks of age. At higher stimulus velocities (40 degrees/s) symmetry was reached at about 12 weeks of age or even much later (80-120 degrees/s).