[The dynamics of the immune status indices in conditions of cranio-cerebral trauma on background of a streptozotocin-induced diabetes mellitus in experiment].

Peculiarities of changes in the immune status in a cranio-cerebral trauma (CCT) on a background of a streptozotocin-induced diabetes mellitus (DM) were studied up. After CCT there are noted the inhibition of predominantly cellular link of immunity, accompanied by reduction of the CD3+ lymphocytes quantity, as well as lymphocytes of the main subpopulations CD4+ and CD8+, CD16+, reduction of the neutrophils phagocytic activity, a complement titer enhancement. Experimental CCT do not cause the essential changes in a CD19+ lymphocytes quantity. The CCT modelling on a background of a streptozotocin-induced DM causes the immune deficiency deepening, in a cellular and humoral links together, significant reduction of activity of a phagocytosis system and complement as well.

[Protective effect of cresacin in D-galactosamine-induced acute experimental hepatitis]. / Zakhysna diia krezatsynu za hostroho eksperimental'noho hepatytu, indukovanoho D-halaktozaminom.

Antioxidant properties of cresacin were studied on the model of galactosamine hepatitis and on the isolated liver cells of white male rats. It has been shown, that cresacin in a dose of 20 mg/kg effectively inhibits the processes of lipid peroxidation induced by hepatotoxin. Cresacin also normalized some components of fermentative and non-enzymatic antioxidant system. In particular the indexes of the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and the level of reduced glutathione, total phospholipids and ascorbic acid was increased for certain. In vitro, on the isolated hepatocytes, cresacin showed the dose-dependent antioxidant effect. This fact is confirmed by its property to inhibit the speed of formation of the malonic dialdehyde in the incubation medium.

[Tris(2-hydroxyethyl)ammonium ortho-cresyl acetate inhibits oxidative modification of the low density lipoproteins]. / Tris(2-oksiétil)ammonii ortokrezoksiatsetat ingibiruet okislitel'nuiu modifikatsiiu lipoproteinov nizkoi plotnosti.

Oxidative modification of low-density lipoproteins (LDLP) may play an important role in the pathogenesis of atherosclerosis. The work deals with the study of the inhibiting effect of tris(2-hydroxyethyl)ammonium orthocresoxyacetate (cresacin) on Cu+2-induced oxidation of human LDLP in vitro. The degree of LDLP oxidation was judged according to the accumulation of diene conjugates in the incubation medium. Inhibition of the degree of LDLP oxidation was found to be dependent on the dose of cresacin. The level of products forming during LDPL oxidation and reacting with thiobarbituric acid was also determined. Like in the previous case, the dose-dependent effect of inhibition of LDLP peroxidation by cresacin was also observed. It is concluded that cresacin is an effective inhibitor of LDLP oxidative modification in vitro.

[Role of the antioxidant system in the pathogenesis of toxic hepatitis]. / Rol' antioksidantnoi sistemy v patogeneze toksicheskogo gepatita.

Four subcutaneous administrations of 2 g/kg of tetrachloromethane to albino rats inhibited the hepatic activity of superoxide dismutase, catalase, glutathione peroxidase, reduced the concentrations of tocopherol, retinol, ascorbic acid, glutathiones, decreased the plasma level of ceruloplasmin and the total antioxidative activity of liver tissue. The magnitude of changes in antioxidative parameters depended on the severity of hepatocytic destruction.

[Status of the antioxidant, monooxygenase and humoral immune system of the body in d-galactosamine hepatitis]. / Stan antyoksydovoï, monooksihenaznoï ta humoral'noï system organizmu pri d-halactozaminovomu hepatyti.

Galactosamine model of toxic hepatitis in rats which, as to its morphobiochemical picture, is considered adequate to human hepatitis was used to study the role of disturbances of the functional state of antioxidant and monooxygenase systems as well as humoral area of the systems of immune protection in pathogenesis of the given disease. It is shown that most components of enzymatic and nonenzymatic antioxidant system (superoxide dismutase, catalase, glutathione peroxidase, restored glutathione, phospholipids) are considerably inhibited by the toxin effect. At the same time content of ceruloplasmin is increased in the blood plasma. Considerable disturbances are also observed in the humoral chain of the immune system (content of immunoglobulins and circulating immune complexes varies) and in the processes of microsomal oxidation.

[The acetylcysteine correction of disorders of the oxidative processes in a toxic liver lesion]. / Korrektsiia atsetiltsisteinom narushenii okislitel'nykh protsessov pri toksicheskom porazhenii pecheni.

The damage of the liver in albino rats induces by CCl4 was followed by the disorders in the free radical, microsomal, and mitochondrial oxidation. The administration of acetylcysteine to the animals in a dose of 500 mg/kg 3 times a day resulted in normalization of chemiluminescence parameters of the liver tissue, the rate of oxygen consumption by microsomes and mitochondria of the hepatocytes, and in normalization of the succinatdehydrogenase, cytochromooxidase, H-ATP-ase activity and ATP concentration.

[Correction of disorders of oxidative processes in toxic liver injury using enterosorption]. / Korektsiia enterosorbtsiieiu porushen' okysniuval'nykh protsesiv pry toksychnomu urazhenni pechinky.

Liver injury with tetrachloromethane in white rats is accompanied by accumulation of hydroperoxides in the blood plasma and liver tissue and suppression of antioxidant system (the activity of superoxide dismutase, catalase, glutathione peroxidase, contents of SH-groups and total phospholipids drastically decreases). Ceruloplasmin level in plasma, on the contrary, increases. Enterosorbent SUGS-E in a dose of 19 g/kg partially normalizes violated balance between the activity of free radical processes and state of the antioxidant protection system.

[Antioxidant effect of dimethyl sulfoxide in acute liver injury by carbon tetrachloride]. / Antiokislitel'noe deistvie dimtilsul'foksida pri ostrom porazhenii pecheni tetrakhlormetanom.

After subcutaneous administration of dimethyl sulfoxide into rats with toxic, tetrachloromethane-produced impairment of liver tissue, lipid peroxidation was effectively inhibited in hepatocytes and the state of anti-oxidation system was improved. Antioxidative properties of dimethyl sulfoxide were shown also in vitro.

[Lipid peroxidation and impairement in erythrocytic properties in patients with ulcer disease before and after surgery]. / Perekysne okyslennia lipidiv i porushennia vlastyvostei erytrotsytiv u khvorykh na vyrazkovu khvorobu do i pislia operatsii.

The state of peroxide oxidation of lipids (POL) in the erythrocytes before and after the operation was studied. In patients with ulcer disease, the correlation between POL activity and changes in erythrocytic properties was noted. At the period of disease exacerbation, activation of POL processes in erythrocytes, suppression of the antioxidation protection (AOP), impairement in hemorheologic indices (increase in aggregation of erythrocytes, their resistance to acid hemolysis, reduction in their capacity for deformation) were revealed. Pronouncement of the pathologic changes after the operation depended on a background state of POL and AOP. In ulcer disease, the impairment in POL/AOP balance caused the development of postoperative complications. To prevent the development of complications, the administration of antioxidants is expedient.

[The effect of acetylcysteine on the antioxidant system in an experimental toxic lesion of the liver]. / Vliianie atsetiltsisteina na antioksidantnuiu sistemu pri éksperimental'nom toksicheskom porazhenii pecheni.

The liver damage by CCl4 in albino rats was followed by a drastic activation of lipid peroxidation processes and suppression of most components of the antioxidant system. The administration of acetylcysteine to the affected animals exerted the positive effect which manifested itself in a decrease of lipid peroxidation products content in the blood plasma and liver and in the partial normalization of the antioxidant protection system.

[Status of the free radical oxidation and antioxidant system in rats with toxic liver damage; effect of tocopherol and dimethylsulfoxide]. / Stan vil'noradykal'noho okislennia i antyoksydantovoï systemy u shchuriv z toksychnym urazheniiam pechinky; vplyv alpha-tokoferolu i dymetylsul'foksydu.

Carbon tetrachloride injected to white rats during four days in the dose of 2 g/kg drastically activates intensity of free radical lipid oxidation and induces impairment of the antioxidant system inhibition of the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, a decrease of SH-groups and general plasma ceruloplasmin level and total phospholipids in the liver. The greatest changes are observed by the 7th day. A complex use of tocopherol (30 mg/kg) and dimethyl-sulphoxide produce partial or complete normalization of all the above mentioned values. It is concluded that the optimization of the protective action of the antioxidant system requires a complex use of water and liposoluble antioxidants.

[Erythrocyte function in patients with complicated peptic ulcer]. / Funktsional'noe sostoianie éritrotsitov u bol'nykh s oslozhnennoi iazvennoi bolezn'iu.

A study of 63 patients with uncomplicated forms of ulcer of the duodenum revealed an increased aggregation activity of erythrocytes, reduction of their capacity to deformation, abnormal stability to oxygen hemolysis, increase of lipid peroxidation in erythrocytes. These disorders of the functional state of erythrocytes depended on the character of complication of the ulcer disease and are related to activation of the processes of lipid peroxidation. Antioxidant drugs are indicated.