ABSTRACT
Intercropping fodder plants with medicinal plants, in addition to enhancing productivity, can remarkably reduce the population of weeds, pests and diseases and for naturally meeting of livestock medicinal needs. Two experiments were conducted to evaluate biological yield, essential oil (EO) composition and yield of sweet basil (Ocimum basilicum L.) treated with N2 fixing bacteria in additive intercropping with forage maize during the 2018 and 2019. Treatments were arranged in factorial split-plot-in time in randomized complete block design with three replications. The factors were 100% chemical fertilizer (N), N2 fixing bacteria (Azospirillum brasilense and Azotobacter chroococcum), integration of N2 fixing bacteria + 50% nitrogen chemical fertilizer and control. The cropping pattern factor included of sole cropping basil and the additive intercropping of maize + 25% basil, maize + 50% basil, maize + 75% basil, and maize + 100% basil. The results indicated that the highest essential oil yield (30.8 kg ha-1) and essential oil percentage (0.75%) were obtained in sole cropping with A. brasilense and A. chroococcum + 50% chemical nitrogen fertilizer application in second harvest in 2019. In both cropping systems, the N2 fixing bacteria application significantly increased fresh and dry yield and land equivalent ratio (LER) as compared to control plants. In both years of experiments could remarkably vary depending on type of treatment. In both years, eight constituents including methyl chavicol (17.24-51.28%), Z-citral (neral) (8.33-24.3%), geranial (10.2-31.3%), (E)-caryophyllene (1.05-5.64%), α-trans-bergamotene (0.53-1.7%), α-humulene (0.4-1.69%), germacrene-D (0.2-1.88%), and (Z)-α- bisabolene (1.16-3.86%) were the main constituents of EO. The highest content of methyl chavicol was found through sole cropping of sweet basil with nitrogen chemical fertilizer followed by sole cropping of sweet basil with an integration of A. brasilense and A. chroococcum + 50% nitrogen chemical fertilizer in 2018 and 2019. Intercropping system and N2 fixing bacteria can be effective in reducing chemical fertilizer consumption and environmental pollution and achieving the sustainable agriculture goals.
ABSTRACT
As a potent herbicide capable of contaminating water and soil environments, paraquat, which is still widely used worldwide, is toxic to mammals, algae, aquatic animals, etc. Paraquat was loaded on novel nanoparticles composed of pectin, chitosan, and sodium tripolyphosphate (PEC/CS/TPP). The size, polydispersity index, and ζ potential of nanoparticles were characterized. Further assessments were carried out by SEM, AFM, FT-IR, and DSC. The encapsulation was highly efficient, and there was a delayed release pattern of paraquat. The encapsulated herbicide was less toxic to alveolar and mouth cell lines. Moreover, the mutagenicity of the formulation was significantly lower than those of pure or commercial forms of paraquat in a Salmonella typhimurium strain model. The soil sorption of paraquat and the deep soil penetration of the nanoparticle-associated herbicide were also decreased. The herbicidal activity of paraquat for maize or mustard was not only preserved but also enhanced after encapsulation. It was concluded that paraquat encapsulation with PEC/CS/TPP nanoparticles is highly efficient and the formulation has significant herbicide activity. It is less toxic to human environment and cells, as was evidenced by less soil sorption, cytotoxicity, and mutagenicity. Hence, paraquat-loaded PEC/CS/TPP nanoparticles have potential advantages for future use in agriculture.
