ABSTRACT
A modified method for the detection of argyrophilic nucleolar organizer regions (AgNORs) is described. By combination of the original silver staining with a Feulgen staining at the same specimen both the AgNORs and the nuclei of the cells under study are sufficiently contrasted, so that a partial automated counting and measurement of these structures can be done using a computerized microscope image analysis system.
Subject(s)
Nucleolus Organizer Region/ultrastructure , Rosaniline Dyes , Animals , Astrocytoma/ultrastructure , Coloring Agents , Glioblastoma/ultrastructure , Histocytochemistry , Humans , In Vitro Techniques , Liver/ultrastructure , Rats , Silver , Staining and Labeling , Urinary Bladder Neoplasms/ultrastructureABSTRACT
Computer-assisted image analysis was used to measure nucleolar organizer regions (NORs) of 22 astrocytomas and glioblastomas. Image analysis provides reproducible information about number and size of NORs together with further karyometric data, which can be compared and processed with other patient-related data. Our study exhibits a statistical relationship between number and size of NORs and malignancy of the measured gliomas.
Subject(s)
Astrocytoma/ultrastructure , Brain Neoplasms/ultrastructure , Glioma/ultrastructure , Image Processing, Computer-Assisted , Nucleolus Organizer Region/ultrastructure , Humans , Karyotyping , Pilot Projects , Staining and LabelingABSTRACT
The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same slide. The changes of extinction measurements caused by the changes of slide thickness and of tissue inhomogeneity could be lowered using this method.
