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Nucleotide excision repair in nuclear extracts from Xenopus oocytes.

Ackerman, E J; Koriazova, L K; Saxena, J K; Spoonde, A Y.

Methods Mol Biol ; 113: 347-55, 1999.

Article in English | MEDLINE | ID: mdl-10443433

Subject(s)

Cell Nucleus/metabolism , DNA Damage , DNA Repair , DNA/genetics , Oocytes/metabolism , Pyrimidine Dimers/metabolism , Animals , Cell Fractionation/methods , DNA/isolation & purification , DNA/metabolism , DNA Replication , DNA, Viral/genetics , Electrophoresis, Agar Gel/methods , Endodeoxyribonucleases/metabolism , Female , Multienzyme Complexes/metabolism , N-Glycosyl Hydrolases/metabolism , Plasmids/genetics , Plasmids/metabolism , Plasmids/radiation effects , Ultraviolet Rays , Xenopus laevis

[Development of monoclonal antibody-based immunoenzyme kit for detection of human thyrotropin hormone]. / Razrabotka immunofermentnogo nabora dlia opredeleniia tireotropnogo gormona cheloveka na osnove monoklonal'nykh antitel.

Sigal, E R; Koriazova, L K; Kovalevskaia, G I; Pozdniakova, T A; Kandyba, E I; Kuznetsov, V A; Vasilov, R G.

Klin Lab Diagn ; (4): 21-4, 1997 Apr.

Article in Russian | MEDLINE | ID: mdl-9221072

ABSTRACT

An enzyme immunoassay kit based on monoclonal antibodies (Mab) has been developed for measuring thyroid-stimulating hormone in human serum and plasma. Laboratory trials demonstrated its high sensitivity (3.1 microIU/ml), specificity, reliability, and accuracy. The analysis is simple and rapid (2.5 h), which is convenient for clinical use. The kit permits differentiation between normal subjects and patients with primary hypo- or hyperthyroidism and helps monitor the treatment efficacy. Comparison with the Amerlite TSH-60 kit, carried out on 357 sera, showed a high correlation coefficient (0.96) between the two kits.

Subject(s)

Immunoenzyme Techniques , Reagent Kits, Diagnostic , Thyrotropin/blood , Antibodies, Monoclonal , Diagnosis, Differential , Humans , Hyperthyroidism/diagnosis , Hypothyroidism/diagnosis , Sensitivity and Specificity

[Preparation of appropriate diagnostic systems of monoclonal antibodies based on human thyroid hormone, and their use in clinical practice]. / Poluchenie prigodnykh dlia diagnosticheskikh sistem monoklonal'nykh antitel k tireotropnomu gormonu cheloveka i ikh ispol'zovanie v klinicheskoi praktike.

Koriazova, L K; Kovalevskaia, G I; Sigal, E R; Pozdniakova, T A; Petrova, E N; Samoilova, V E; Vasilov, R G.

Biull Eksp Biol Med ; 118(10): 433-5, 1994 Oct.

Article in Russian | MEDLINE | ID: mdl-7532463

Subject(s)

Antibodies, Monoclonal , Hyperthyroidism/diagnosis , Hypothyroidism/diagnosis , Thyroid Hormones/immunology , Animals , Epitopes/immunology , Humans , Immunoenzyme Techniques , Mice , Mice, Inbred BALB C , Radioimmunoprecipitation Assay

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