The Role of Dimethoate and UV-B on Skin of Wistar Rats.

BACKGROUND/AIM: The pesticide dimethoate (O-dimethyl-S- Nmethylcarbamoylmethyl phosphorodithioate) is able to induce severe acute toxicity in living organisms. The aim of this study was to evaluate the effects of ultraviolet radiation, alone or combined with exposure to dimethoate, on the rat skin. MATERIALS AND METHODS: A total of 38 Wistar female rats (Rattus norvegicus albinus), were distributed into four groups: A (n=9) control group, B (n=10) exposed to ultraviolet-B radiation (UV-B), C (n=10) exposed to UV-B followed by application of dimethoate (UV-B+AGRO) and group D (n=9) exposed to dimethoate (AGRO). Histological examination of the tissues, as well as immunohistochemistry for cleaved caspase 3, Ki-67 and COX-2 expression were performed to all groups. RESULTS: Animals submitted to UV-B exhibited hyperkeratosis with moderate cell atypia. Regarding exposure to UV-B+AGRO, the animals presented hyperkeratosis and atrophy, whereas in animals exposed to AGRO, only atrophy was noticed. The immunohistochemical results on skin revealed that UVB, AGRO and UVB+AGRO decreased cleaved caspase 3 and Ki-67 expression when compared to the control group (p<0.05). COX-2 expression decreased to UVB or AGRO groups compared to controls (p<0.05). CONCLUSION: UV-B or AGRO exposure is able to induce histopathological changes and altered expression of cleaved caspase-3 and Ki-67 in rat skin, thus being categorized as a risk condition for skin carcinogenesis.

Caracterização do atendimento às vítimas de acidentes de trânsito em um serviço público de emergência / Characterization of the service to the victims of transit accidents in a public emergency service

Objetivo: caracterizar as vítimas de acidentes de trânsito em um serviço público de emergência, enfocando os acidentes que envolvem motocicleta. Método: estudo descritivo, transversal, de abordagem quantitativa, com análise documental de dados secundários, desenvolvido em um Hospital Universitário (HU). Resultados: a maior parte das vítimas foi do sexo masculino (76,4%). Com relação à idade, predominaram, neste estudo, indivíduos com idade superior a 20 anos (50%), seguidos pelos que tinham de zero a 15 (30,3%). Entre todos os casos, 1,9% das vítimas foram a óbito. Conclusão: Tendo em vista a abrangência da Rede PEBA, os resultados servirão de subsídio na implementação da Rede Interestadual de Atenção à Saúde do Vale do Médio São Francisco, podendo ainda ser implantadas ações educativas específicas de promoção e prevenção de acidentes no trânsito. Estas devem focar, prioritariamente, o perfil mais atingido, na perspectiva de conscientizá-lo para a redução do fenômeno.

Análise espacial dos acidentes de trânsito atendidos na urgência de um hospital universitário / Space analysis of transit accidents attended at the emergency of a university hospital

Objetivo: caracterizar os Acidentes de Trânsito (AT’s) atendidos na urgência. Método: estudo exploratório, de abordagem quantitativa, de dados retrospectivos e de base populacional. Os dados foram coletados a partir de um livro de registro utilizado pelo setor de epidemiologia de um Hospital Universitário e georreferenciados pelo método de Kernel. Resultados: a cidade de Petrolina apresentou o maior número nos índices de acidentes de trânsito (83,6%). O bairro Centro teve maior frequência (8,5%), predominância do sexo masculino, faixa etária do sexo masculino entre os 16 aos 45 anos, com 78,1% dos acidentes. Percebeu-se menor ocorrência no sábado e no domingo, com 8,3% e 8,5% casos, respectivamente. Conclusão: quanto ao Contorno de Kernel, identificaram-se dois pontos críticos para o acidente de trânsito: o Centro e o bairro João de Deus, sendo a BR 401 a de maior ocorrência para o acidente de trânsito.

Perfil dos usuários atendidos no serviço de emergência em um Hospital Universitário em Pernambuco / Perfil de los usuarios atendidos en el servicio de urgencias de un hospital universitario de Pernambuco / Profile of users seen in the emergency service at a university hospital in Pernambuco

Objetivo: Conhecer o perfil dos usuários atendidos na sala vermelha de um Hospital Universitário de Pernambuco. Métodos: Estudo descritivo, exploratório e quantitativo, realizado no setor do atendimento de emergência de um Hospital Universitário de Pernambuco, utilizando como fonte de dados o Livro de Registro dos atendimentos diários, coletados de janeiro a junho de 2013. Os dados foram organizados em uma planilha no programa Microsoft Office Excel® 2010. Logo após, foram apresentados em gráfico e tabelas, confrontando os resultados com a literatura. Resultados: A maioria dos pacientes atendidos é do sexo masculino, entre 20 e 29 anos, procedentes da mesma cidade, onde está localizado o hospital, e vítimas de causas externas, principalmente acidentes motociclísticos. Conclusões: A população economicamente ativa foi a mais atingida pelas causas externas, o que pode desencadear despesas elevadas para o Sistema Único de Saúde (SUS). Espera-se que o estudo sirva de subsídio para implantações de políticas públicas municipais e regionais.

Objetivo: Conocer el perfil de los alumnos en el Salón Rojo del Hospital de la Universidad de Pernambuco. Métodos: Estudio descriptivo, exploratorio y cuantitativo, realizado en el sector de respuesta de emergencia del Hospital de la Universidad de Pernambuco, se utilizando como fuente de datos el libro de registro de asistencia diaria, recogido de enero a junio de 2013. Los datos fueron organizados en una hoja de cálculo de Microsoft Office Excel® 2010. Después, se presentan en el gráfico y en las tablas, haciendo una comparación de los resultados con la literatura. Resultados: La mayoría de los pacientes atendidos son del sexo masculino, entre 20 y 29 años, de la misma ciudad, donde se encuentra el hospital, y víctimas de causas externas, principalmente los accidentes de motocicleta. Conclusiones: La población económicamente activa fue la más afectada por causas externas, que pueden desencadenar un alto costo para el Sistema Único de Salud (SUS). Se espera que el estudio sirva como un subsidio para los despliegues municipales y regionales de políticas públicas.

Objective: To be aware of the profile of the patients cared in the emergency room of the University Hospital of Pernambuco. Methods: DA descriptive, exploratory and quantitative study. We carried out this research in the emergency room of the University Hospital of Pernambuco, usinged as a source the visitor registration book, with datacollected collected from January to June 2013 sector. The selected variables were organizedin a Microsoft Office Excel 2010 spreadsheet in Microsoft Office Excel 2010 program. Shortly after, those data were presented in graphic and tables comparing the results with the literature. Results: The majority of cared patients seen is male, ranging from 20 to 29 years old, coming from the same city where the hospital is located, and victims of external causes, especially motorcycle accidents. Conclusions: The economically active population was the most affected by external causes, which may triggering higher costs for the Unified Health System (SUS). We hoped that this study will serves as an input for the implementation deployments of municipal and regional health public policiesy.

Induction of neoplastic cells in rat skin.

PURPOSE: To investigate the induction of neoplastic lesions under the action of ultraviolet B radiation (UVR-B) and dimethyl benzanthracene (DMBA). METHODS: Forty Wistar rats were assigned to four groups (ten animals each), according to the procedure: group A received UVR-B irradiation, group B received topic DMBA, group C, UVR-B+DMBA and group D as control, observed for ten weeks. In the tenth week they went through a skin biopsy and histopathological study. The average thickness of the epidermis was calculated and evaluated statistically. RESULTS: Macroscopic lesions in group B were more of inflammatory kind compared to group A. Group C presented more injuries with neoplastic features than the others (p<0.01). Histologically there was a significant increase in thickness of the epidermis of all groups compared to control, however the greatest thickness measures occurred in Group C (p<0.01). CONCLUSIONS: The population exposed to ultraviolet B radiation is subject to suffer skin lesions that can develop into cancer. The association with hydrocarbons as the dimethyl benzanthracene increases the possibility of malignancy. May not be clinically evident determine when a solar keratosis ends and when a CEC begins. For this reason, histological study associated with health education prompting the early and irreversible injury prevention is necessary.

Induction of neoplastic cells in rat skin

To investigate the induction of neoplastic lesions under the action of ultraviolet B radiation (UVR-B) and dimethyl benzanthracene (DMBA). METHODS: Forty Wistar rats were assigned to four groups (ten animals each), according to the procedure: group A received UVR-B irradiation, group B received topic DMBA, group C, UVR-B+DMBA and group D as control, observed for ten weeks. In the tenth week they went through a skin biopsy and histopathological study. The average thickness of the epidermis was calculated and evaluated statistically. RESULTS: Macroscopic lesions in group B were more of inflammatory kind compared to group A. Group C presented more injuries with neoplastic features than the others (p<0.01). Histologically there was a significant increase in thickness of the epidermis of all groups compared to control, however the greatest thickness measures occurred in Group C (p<0.01). CONCLUSIONS: The population exposed to ultraviolet B radiation is subject to suffer skin lesions that can develop into cancer. The association with hydrocarbons as the dimethyl benzanthracene increases the possibility of malignancy. May not be clinically evident determine when a solar keratosis ends and when a CEC begins. For this reason, histological study associated with health education prompting the early and irreversible injury prevention is necessary.