[A study of parietal gastrointeatinal microflora of rats after oral administration of probiotics].

The article is dedicated to one of the topical problems of medical microecology, possibility of modification of parietal gastrointestinal microflora by oral administration of various probiotics, and evaluation of the effects of such modification. The results of the study show that parietal gastrointestinal microflora can be modified by oral administration of probiotics.

[Study of the parietal microflora in the rat intestine].

The article deals with the adaptation of the existing method of the isolation of microorganisms from parietal mucin of the intestine of experimental animals (rats) with a view to ensure the methodological uniformity of investigations. The effectiveness of the method of sparing disintegration of parietal mucin taken from the mucous membrane of the gastrointestinal tract, specially developed for human biomaterial, and its adequacy for microbiological investigations of the digestive organs of rats have been confirmed. A certain similarity between the microbiocenosis of the parietal mucin in the intestine of humans and rats has been established.

[An analysis of the strain homogeneity of parietal biotopes of the gastrointestinal tract]. / Analiz shtammovoi obshchnosti pristenochnykh biotopov zheludochno-kishechnogo trakta.

The lumen and parietal biotopes of the human gastrointestinal tract were investigated, i.e. the antibiotic sensitivity of strains E. coli and Enterococcus faecalis residing in them were comparatively analyzed. Certain isolation degrees not only of the parietal and fecal biotopes but also of certain micro-regions in the intestine wall were detected. The obtained data confirm a previously made assumption on the cluster distribution of microorganisms in the parietal mucin. The distinct strain differences between the parietal and fecal biotopes are not enough to completely extrapolate the available characteristics of the lumen microflora on the parietal microbiocenose. The above stated underlines the necessity in purpose-oriented research of parietal microbe populations of "internal" biotopes.

[The parietal mucin microbial population in different sections of the human gastrointestinal tract]. / Mikrobnye soobshchestvo pristenochnogo mutsina razlichnykh otdelov zheludochno-kishechnogo trakta cheloveka.

Microbiological examinations of the human-colon parietal mucin were made in different sections of the human gastrointestinal tract (GIT). Biopsy samples of the human colon mucus tunic were used as the research material. Convincing data were found by research on the importance of the anatomic-and-morphological factor in shaping-up of microbiocenosis of the colon-wall parietal mucin. It was established that, depending on a GIT section, not only the quantitative and qualitative composition of normal microflora but also the nature of inter-microbe interactions are subject to changes. In particular, biopsy materials of the small-intestine parietal mucin are much different from that of the colon. The colon section from the ascending colon to the sigmoid colon has, with some exceptions, an identical microbial composition. At the same time, the rectum is significantly different from other colon sections. The results are suggestive of the below cluster-type pattern of parietal biological material: microorganisms are clustered as small domains with certain specific and quantitative compositions. It was established that, although feces and parietal mucin have a similar species composition of microorganisms, still, there is a number of essential differences between them in as far as the frequency ratio and the microorganism concentration are concerned, which signifies a certain degree of isolation of the above biopsy materials. Finally, a certain isolation degree of the feces biopsy materials and of the parietal ones was established.

[A study of the human parietal gastrointestinal tract microflora in health and disease]. / Issledovanie pristenochnoi mikroflory zheludochno-kishechnogo trakta u cheloveka v norme i pri patologii.

An original technique was used to investigate the human colon mucin in different pathologies of gastrointestinal tract. The microbial pattern of the biotope in question was found to be dependable on a morphological-and-functional condition of the intestinal wall. It is noteworthy, that not only its quantitative and qualitative composition but also the nature of the inter-microbe relations and the "specific content" of microbes are subject to changes. A correlation was established between the studied characteristics of the human parietal gastrointestinal biotope and the biochemical blood parameters. The results revealed a new chain in the pathogenesis of disbiotic processes occurring in the colon mucous tunic. The diagnostic value of microbiological parameters of the human colon mucous tunic is under discussion.

[Parital microflora of human intestine]. / Issledovanie pristenochnoi mikroflory kishechnika cheloveka.

The modified method of the microbiological study of parietal mucin has been developed. The proposed method makes it possible to evaluate the parietal microflora of the intestine. The advantages of using physiological saline and Hanks' solution as medium ensuring the storage and self-thinning of mucin have been proved. The optimum time of making the microbiological study of intestinal mucin has also been determined.