ABSTRACT
During the last decade brain organoids have emerged as an attractive model system, allowing stem cells to be differentiated into complex 3D models, recapitulating many aspects of human brain development. Whilst many studies have analysed anatomical and cytoarchitectural characteristics of organoids, their functional characterisation has been limited, and highly variable between studies. Standardised, consistent methods for recording functional activity are critical to providing a functional understanding of neuronal networks at the synaptic and network level that can yield useful information about functional network phenotypes in disease and healthy states. In this study we outline a detailed methodology for calcium imaging and Multi-Electrode Array (MEA) recordings in brain organoids. To illustrate the utility of these functional interrogation techniques in uncovering induced differences in neural network activity we applied various stimulating media protocols. We demonstrate overlapping information from the two modalities, with comparable numbers of active cells in the four treatment groups and an increase in synchronous behaviour in BrainPhys treated groups. Further development of analysis pipelines to reveal network level changes in brain organoids will enrich our understanding of network formation and perturbation in these structures, and aid in the future development of drugs that target neurological disorders at the network level.
ABSTRACT
Self-assessment of health is the one out of the most important characteristics, which gives an idea about the health of the respondent as a psychophysiological continuum. The purpose of this study is to establish the relationship between indices of self-rated health and characteristics of the psychological well-being. Sample 344 young adults - students at an average age of 20.9 ± 1.6 years. Methods. "Self-assessment of health" questionnaire was used; MOSSF36 questionnaire and "Psychological wellbeing scale" developed by K. Riff. Respondents were divided into groups: (1) without chronic diseases; (2) having one or more chronic diseases of any etiology. Results. The presence of chronic diseases was shown to negatively effect on the self-estimation of physical and psychological state. The significant positive correlation (p <0,001) between the perceived health and subjective well-being was found. Healthy individuals perceive the state of their own physical health and psychological wellbeing as unrelated to each other characteristics. In contrast, in cases with even mild chronic health problems there is pronounced the relationship between somatic and psychological health, which forms a complex of physical and psychological factors determining the general state of a person.
