ABSTRACT
The aim of this work was to examine the content of arylhydrocarbon receptor nuclear translocator (ARNT) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of ARNT in age-dependent changes in the number of fibroblasts in the dermis. ARNT, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for ARNT in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of ARNT positive fibroblasts in dermis is increased sufficiently since 41 year old until 60-85 years old group. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Age-related changes in the content of ARNT in fibroblasts is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts the dermis.
Subject(s)
Aging/metabolism , Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism , Skin Aging , Skin/metabolism , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Dermis/cytology , Dermis/metabolism , Fetus/metabolism , Fibroblasts/metabolism , Humans , Infant , Infant, Newborn , Skin/cytology , Young AdultABSTRACT
The aim of this work was to examine the content of heat shock protein 90 (HSP90) in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of HSP90 in age-dependent changes in the number of fibroblasts in the dermis. HSP90, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for HSP90 in the dermis is not changed from 20 weeks of development to 20 years old. Percent of HSP90 positive fibroblasts in dermis is decreased from 21 to 60 years old. From 61 year, the number of HSP90 positive fibroblasts in dermis is increased. Age-related changes in the number of HSP90 positive fibroblasts is not statistically associated with an age-related decrease in a total number and percent of PCNA positive fibroblasts the dermis.
Subject(s)
Aging , Dermis/cytology , Fibroblasts/metabolism , HSP90 Heat-Shock Proteins/metabolism , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Middle Aged , Pregnancy , Young AdultABSTRACT
The aim of this work was to examine the content of Yes-associated protein (YAP) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of YAP in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. YAP, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for YAP in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of YAP positive fibroblasts in dermis is increased sufficiently since 41 years old until 60-85 years old group. The content of YAP in blood microvessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old. Age-related changes in the content of YAP in fibroblasts and blood microvessels is not statistically associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.
Subject(s)
Adaptor Proteins, Signal Transducing , Endothelial Cells , Skin Aging , Transcription Factors , Adaptor Proteins, Signal Transducing/genetics , Adaptor Proteins, Signal Transducing/metabolism , Adult , Aged , Aged, 80 and over , Aging , Dermis/cytology , Dermis/metabolism , Female , Fibroblasts/metabolism , Humans , Middle Aged , Pregnancy , Skin Aging/physiology , Transcription Factors/genetics , Transcription Factors/metabolism , YAP-Signaling ProteinsABSTRACT
The aim of this work was to examine the content of transcription coactivator with PZD-binding motif (TAZ) in fibroblasts and blood vessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TAZ in age-dependent changes in the number of fibroblasts and blood vessels in the dermis. TAZ, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TAZ in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of TAZ positive fibroblasts in dermis is increased since 41 years old until 60-85 years old group. The content of TAZ in blood vessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old followed by an increase from 41 years old. From 61 to 85 years of life, content of TAZ in dermal vessels was not differ from those in 41-60 age group. Age-related changes in the content of TAZ in fibroblasts and blood vessels is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.
Subject(s)
Aging , Fibroblasts/cytology , Protein Serine-Threonine Kinases/genetics , Skin Aging/genetics , Trans-Activators/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Dermis/blood supply , Dermis/cytology , Female , Hippo Signaling Pathway , Humans , Infant , Infant, Newborn , Middle Aged , Pregnancy , Signal Transduction , Transcriptional Coactivator with PDZ-Binding Motif Proteins , Young AdultABSTRACT
The aim of our work was to examine content of serine-arginine protein kinase 1 (SRPK1) in human dermis at different ages (from 20 weeks of pregnancy to 85 years old). SRPK1, proliferating cells nuclear antigen (PCNA ), endothelial marker CD31 were detected in sections of the skin by indirect immunohistochemistry. Results showed, that content of SRPK1 in dermal fibroblasts was increased form antenatal period to 20 years of life followed by a decrease until 61-85 years period. SRPK1 content in dermal blood vessels is slowly gradually increased from antenatal period to 61-85 age interval. The number of fibroblasts and their proliferative activity, the number of CD31 positive blood vessels in dermis were decreased from antenatal period to 61-85 years period of life. Age-dependent decrease in SRPK1 in dermal fibroblasts from 20 years is associated with a reduction in the number and proliferative activity of fibroblasts. Age-related increase in SRPK1 content in dermal blood vessels is associated with a diminishing of the number of blood vessels. Hence, it can be supposed that SRPK1 has different actions on proliferation of differ components of dermis during aging.
Subject(s)
Dermis/enzymology , Protein Serine-Threonine Kinases/metabolism , Adolescent , Adult , Age Factors , Aged , Aged, 80 and over , Aging/metabolism , Child , Child, Preschool , Female , Fetus/enzymology , Fibroblasts/enzymology , Humans , Infant , Infant, Newborn , Male , Middle Aged , Platelet Endothelial Cell Adhesion Molecule-1/analysis , Pregnancy , Proliferating Cell Nuclear Antigen/analysis , Young AdultABSTRACT
In this study, quantitative analysis of inflammatory effectors--mast cells and eosinophils--in derma of people of different ages is performed. The study shows that mast cell quantity in derma increases with age. Eosinophils are rarely observed in human dermis. There are no age-correlated changes of dermal eosinophils quantity observed. Age-correlated dermal fibroblast quantity is established. PCNA+ fibroblast percentage demonstrating their proliferative pool also reliably decreases with age. Results of correlation analysis show that age-correlated increase in mast cells' quantity is reliably correlated with decrease in total number and percentage of PCNA+ fibroblasts in derma. Consequently, age-correlated increase in dermal mast cell may be proposed to be one of the inflammatory and aging mechanisms. Mast cells, whose number increases with aging, may influence dermal fibroblast number with aging.
Subject(s)
Aging/physiology , Dermis/metabolism , Eosinophils/metabolism , Fibroblasts/metabolism , Mast Cells/metabolism , Proliferating Cell Nuclear Antigen/metabolism , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Dermis/cytology , Eosinophils/cytology , Female , Fibroblasts/cytology , Humans , Infant , Infant, Newborn , Male , Mast Cells/cytology , Middle Aged , PregnancyABSTRACT
The aim of this work was the study of age-related changes in the number of CD45+ cells in human dermis. The results showed that the number of CD45+ cells in dermis increased gradually with age. At the same time, a total number of fibroblasts in dermis decreased with age. The number of PCNA+ fibroblasts in dermis showing their proliferative activity was observed to be decreased with the progression of age. The results of the correlation analysis show that the age-related increase of CD45+ cells number is statistically associated with the decrease in the total number of fibroblasts and with the quantity of PCNA+ fibroblasts in dermis through age. Therefore, accumulation of CD45+ cells in human dermis with age may be regarded as a mechanism which brings about inflammatory reaction and emergence of signs of aging. It is also possible that the increased number of bone marrow derived cells has an impact on the age-related decline in the number of fibroblasts in dermis with age.
Subject(s)
Dermis/pathology , Fetus/pathology , Leukocyte Common Antigens/biosynthesis , Skin Aging/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Cell Count , Cell Proliferation , Child , Child, Preschool , Data Interpretation, Statistical , Dermis/embryology , Dermis/growth & development , Dermis/metabolism , Female , Fetus/metabolism , Fibroblasts/metabolism , Fibroblasts/pathology , Humans , Immunohistochemistry , Infant , Infant, Newborn , Male , Middle Aged , Proliferating Cell Nuclear Antigen/biosynthesis , Young AdultABSTRACT
Skin aging is important medical and social problem in modern world. A goal of present work was to estimate changes in the number of fibroblasts and PCNA-positive fibroblasts in human dermis at different ages. Total number of fibroblasts per unit area was counted in slides stained with haematoxylin and eosin. PCNA which is present in cells being in the cell cycle was revealed immunohistochemically. Results showed a decrease in a total number of fibroblasts in dermis through aging. The number of PCNA-positive fibroblasts in dermis is also decreased with progression of age. It may be concluded that age-related decrease in dermal fibroblasts number is associated with diminished proliferation of these cells. Causes which lead to a decrease in proliferation are not clear, and new researches are needed to reveal them.
Subject(s)
Cell Proliferation , Fibroblasts/pathology , Skin Aging/pathology , Skin/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Cell Count , Child , Child, Preschool , Female , Fibroblasts/metabolism , Humans , Infant , Infant, Newborn , Male , Middle Aged , Proliferating Cell Nuclear Antigen/metabolism , Skin/embryology , Skin/growth & development , Skin/metabolism , Young AdultABSTRACT
15 uteri removed surgically because of abruptio placentae (AP) were studied morphologically. AP develops as a process due to a combination of maternal (endometritis, gestosis, adenomyosis), placental (pathology of microvilli, hypercoagulation of venous blood) and hemorheological (thrombosis of placental bed veins, partial blockade of venous circulation) factors. These factors result in the formation of retroplacental hematoma and progressing intrauterine fetal hypoxia.
Subject(s)
Abruptio Placentae/pathology , Uterus/pathology , Abruptio Placentae/etiology , Endometriosis/complications , Endometriosis/pathology , Endometritis/complications , Endometritis/pathology , Female , Fetal Hypoxia/etiology , Fetal Hypoxia/pathology , Hematoma/complications , Hematoma/pathology , Humans , Placenta/blood supply , Placenta/pathology , Placental Circulation , Pre-Eclampsia/pathology , PregnancyABSTRACT
An accelerated method of paraffin embedding of placentas in pathohistological practice is suggested allowing to get micropreparations stained with hematoxylin and eosin and other methods from 500 500 and more blocks 4-5 hours after cutting. This enables to analyze material from groups of risk among children born alive and rapid informing neonatologists and pediatricians. The method is based on rejection of formaldehyde fixation (this excludes a toxic effect on the personnel). Use of acetone combines fixation and dehydration. The reagents usable for technical reasons make the proposed method cost effective.
