[The comparative study of indicators of immunity and state of pro- and antioxidant systems in patients with chronic pyelonephritis of single kidney in active and latent stages of disease].

It is established that in patients with chronic pyelonephritis of single kidney the antibacterial defense of organism and balance between pro- and antioxidant systems are disturbed both in active and latent stages of disease. The results testify the need of organization of comprehensive immunologic and biochemical examination to establish a prognosis of complications and evaluation of effectiveness of applied treatment.

[Free-radical processes in cardiomyopathy and concomitant vibration disease].

Progression from local vibration to cardiovascular pathology in patients with vibration disease is associated with remodeling cardiac and vascular tissues under effect of oxidative stress. Myocardium and nodular tissue are most vulnerable during development of occupational cardiopathy. Alteration of the heart rhythm wave structure is a predictor of remodeling.

[Neutrophils and antioxidant activity of cervical secretion in women with the acute inflammation in the upper section of the reproductive tract]. / Neitrofily i antioksidantnaia aktivnost' tservikal'nogo sekreta u zhenshchin s ostrym vospaleniiem verkhnego otdela genitalii.

The functional activity of neutrophils, peroxidation products and antioxidative activity of cervical secretion in women with the acute inflammatory process in the upper section of the reproductive tract were under study. The most pronounced changes in the function of neutrophils were observed in women with the low antioxidative activity of cervical slime.

[Changes in oxidative processes in the anterior segment of the eye in patients with keratoconus]. / Izmeneniia okislitel'nykh protsessov v perednem otrezke glaza u bol'nykh keratokonusom.

Biochemical parameters of the tears, reflecting oxidative processes in the eyes, were analyzed in 79 patients (146 eyes) aged 19-42 years with progressive and acute keratocone and with long remission of the disease. The data indicate disorders in oxidative processes with predominance of aerobic oxidation over anaerobic in the anterior segment of the eye. Evaluation of lipid peroxidation and antioxidant enzymes showed predominance of prooxidant processes over oxidant. These disorders are related in keratocone and differ in progressive, acute condition, and remission. The greatest changes in the lacrimal oxidative processes are observed during the disease progress and even more so in acute disease. The detected changes in the lacrimal biochemistry in patients with keratocone can be regarded as an important mechanism underlying the dystrophic processes in the cornea.

[Lipid peroxidation and antioxidant system parameters in lacrimal fluid in post-traumatic uveitis]. / Pokazateli perekisnogo okisleniia lipidov i antioksidantnoi sistemy v sleznoi zhidkosti pri posttravmaticheskom uveite.

Lipid peroxidation (LPO) values and activities of antioxidant enzymes, catalase, and superoxide dismutase were studied in the lacrimal fluid (LF) of the damaged and intact eyes of 87 patients with posttraumatic uveitis (PTU). Catalase activity was decreased in sluggish PTU and increased in exacerbation, which is attributed to decompensation of the antioxidant system and its effect on the course of posttraumatic inflammation. Similar changes were detected in LF of paired eyes. Long treatment with antioxidants is recommended; the authors consider that analysis of the paired eye LF cannot be used for control in patients with PTU.

[Lipids in the exhaled air condensate of pneumonia patients]. / Lipidy kondensata vydykhaemogo vozdukha u bol'nykh pnevmoniei.

Lipid composition of exhaled air condensate (EAC) was assessed by thin layer chromatography in patients with pneumonia and normal subjects. In parallel with this, diene conjugates and diene ketones were assessed by spectrophotometry. Berhan's principle was for the first time used for noninvasive obtaining of EAC from each lung separately. Decreased percentage of phospholipids and increased level of free fatty acids in EAC of patients with massive pneumonia may be an evidence of disordered pulmonary surfactant, and an increase of the intermediate products of lipid peroxidation indicate the cause of these changes.

[Modification of the determination of lipid peroxidation products in a reaction with thiobarbituric acid]. / Modifikatsiia opredeleniia produktov perekisnogo okisleniia lipidov v reaktsii s tiobarbiturovoi kislotoi.

The aim of the study was to choose the optimal conditions for spectrophotometry of the blood serum lipid peroxidation (LPO) products by the thiobarbituric acid (TBA) test. LPO products have been measured in trichloroacetic acid filtrate and in lipoprotein sediment by spectrophotometry and fluorometry. The optimal conditions for spectrophotometry of TBA-active LPO products are as follows: the proteins are sedimented with 20% solution of phosphotungstic acid, stabilizing the pH values in 1.3-1.5 range; the incubation temperature is 99-100 degrees C; the optic density of the aqueous extract is measured spectrophotometrically at wavelengths 535 and 580 nm. The suggested method permits spectrophotometry of LPO products without additional extraction with butanol, and its results are compatible with those of fluorometry. The coefficient of convergence variations is 3.42%, the repeatability factor 3.31%.

[Dynamics of alpha1-antitrypsin and alpha2-macroglobulin in patients with acute and chronic pneumonia]. / Dinamika alpha1-antitripsina i alpha2-makroglobulina u bol'nykh ostrotekushchei i zatiazhnoi pnevmoniei.

Content of alpha 1-antitrypsin and alpha 2-macroglobulin was studied in blood serum of patients with acute and chronic forms of pneumonia. Within the first decade of the disease alpha 1-antitrypsin was more distinctly increased in the patients with chronic pneumonia. Stable elevation of alpha 1-antitrypsin correlated with long-term presence of the inflammation symptoms. Content of alpha 2-macroglobulin was slightly decreased during the disease.

[Prevention of stress damage to the body by antioxidants and the beta-blocker Inderal]. / Preduprezhdenie stressornykh povrezhdenii organizma antioksidantami i beta-blokatorom inderalom.

Ulcerous impairments of gastric mucosa and an increase in activity of some enzymes in blood plasma were observed in rats after severe emotional-pain stress (EPS). The activity of cytoplasmic enzymes in blood plasma reached maximal values immediately after EPS and then returned to the initial level within the first day. At the same time, activity of lysosomal enzymes reached the highest values only at the end of the first day after EPS and their increased activity was maintained within 5 days. Ulcerous impairments of stomach as well as destruction of cellular membranes, responsible for an increase in the enzymatic activity in blood plasma, might be prevented by beta-adrenoblocking agent inderal. These data suggested that the impairments were due to the effect of excessive amount of catecholamines. Antioxidants dibunol and OP-6, administered prior to EPS, prevented the impairments caused by stress more effectively as compared with inderal; other antioxidants--alpha-tocopherol acetate and sodium selenite were less effective. The data obtained support the assumption on the leading role of lipid peroxidation in pathogenesis of the stress impairments; these data established that the impairments caused by stress might be prevented using the combination of antioxidants and beta-adrenoblocking agents.