ABSTRACT
Non-producer hamster and virus-producing chicken Rous sarcoma cells contain a complete avian sarcoma virus (ASV)-provirus DNA (pro-DNA). Unlike this, in normal hamster liver DNA to ASV--specific sequences are absent. Moreover, the nuclear chicken Rous sarcoma and chick embryo cell (CEC) DNA preparations contain practically all endogenous chicken virus (ECV)-specific sequences, while the hamster tumor DNA was annealed with only more than half of the ECV-RNA sequences. Thus, the pro-DNA of permissive hosts contains so-called "sarcoma-specific", "common" and "endogenous--specific" nucleotide sequences. The "sarcoma-specific" and "common" sequences are present in the hamster sarcoma pro-DNA, only. Both the pro-DNA of permissive cells and the pro-DNA of non-permissive cells consist of moderately reiterated and unique sequences. The "common" regions of pro-DNA are localized, mainly, in the unique zone, while "sarcoma-specific" and "endogenous-specific" sequences of pro-DNA - in the moderately reiterated and unique zones. The pro-DNA organization of permissive and non-permissive hosts is discussed.
Subject(s)
Avian Sarcoma Viruses/analysis , DNA, Viral/analysis , Animals , Base Sequence , Cricetinae , Genes, ViralABSTRACT
In embryonal human cell culture there was found the biological activity of DNA preparations, obtained from peripheral blood cells of acute leukemia patients, which was manifested in the production of virus-like RNA-containing particles, as evidenced by the radioisotope analysis findings and by the reverse transcriptase content in these particles. Also, a rapid growth of the cultures was noted. The DNA preparations from rhabdomyosarcoma, neurinoma and synovial sarcoma in man would cause only an enhancement of human embryonal cell cultures growth. No production of virus-like particles in the latter was noted.
Subject(s)
DNA, Neoplasm/genetics , Neoplasms/genetics , Retroviridae/genetics , Transfection , Adult , Cells, Cultured , Enzyme Activation , Female , Humans , Leukemia, Myeloid, Acute/genetics , Lung , Neurilemmoma/genetics , RNA-Directed DNA Polymerase/analysis , Rhabdomyosarcoma/genetics , Sarcoma/genetics , Sarcoma, Synovial/genetics , Soft Tissue Neoplasms/genetics , Time FactorsABSTRACT
The nuclear genome of Syrian hamster virrion non-producer tumor contains the Rous sarcoma virus-specific nucleotide sequences. The information complexity of the proviral DNA from its tumor is similar to that for the DNA of Rous chicken sarcoma. The proviral DNA both in the hamster and chicken tumor consists of moderately reiterated and unique sequences. The avian oncornavirus-specific sequences are absent in the DNA of normal hamster tissue.
Subject(s)
DNA, Neoplasm/analysis , DNA, Viral/analysis , Nucleic Acid Hybridization , Sarcoma, Avian/analysis , Animals , Avian Sarcoma Viruses , Base Sequence , Chickens , Chromosomes/analysis , Cricetinae , Genes , MesocricetusABSTRACT
An infectious process was reproduced in the culture of chick embryo cells by means of DNA isolated from Rous chick sarcoma tissue (Carr-Zilber strain). This DNA preparation displays biological activity also in the culture of human embryo diploid cells (HEDC) which is manifested in: 1. discontinuous synthesis of avian oncovirus group-specific antigen; 2. enhancement of proliferative activity and morphological transformation of human cells; 3. continuous presence of virus-specific sequences as revealed by DNA/RNA hybridization. Producing complete oncornavirus by means of DNA isolated from Rous chick sarcoma in HEDC was unsuccessful. DNA preparation from gs negative chick embryo cells shows no infectious activity in HEDC culture.
Subject(s)
Antigens, Viral , Avian Sarcoma Viruses/immunology , Cell Transformation, Neoplastic , DNA, Neoplasm/physiology , Cell Division , Cell Line , Cell Nucleus/ultrastructure , DNA, Viral , Nucleic Acid HybridizationABSTRACT
The infection process was reproduced in the culture of chick embryonal cells by means of: a) DNA isolated from chick Rous sarcoma (Carr-Zilber strain); b) DNA from blood cells of chicks with myeloblastosis (strain A); c) DNA from Rous virus malignified (Prague strain) rat cells (XC). Antigenic properties of the virus, transfected with DNA from chick Rous sarcoma, would differ from the original parental strain (Carr-Zilber RSV), that evidences the possibility of a partial transfer of genetic information in transfection. A suggestion is made on four presumable variants of oncornaviruses transfection by means of tumor cells DNA.
