[Possible mechanisms of synapse formation during ontogenesis]. / Vozmozhnye mekhanizmy formirovaniia sinapsov v ontogeneze.

Electron microscopic investigation of synaptogenesis in the sensomotor cortex and in the caudate nucleus has been performed in the prenatal ontogenesis (16-22 days) and in newborn rats. The first immature synapses are demonstrated to appear beginning on the 16th day of embryogenesis. At the end of the prenatal development and especially in newborn animals desmosome-like, asymmetric and symmetric, mixed and complex forms of the synaptic contacts are revealed. As a result of the analysis performed on the ultrastructural organization of the contacts, a hypothesis explaining mechanisms of development of various elements of the synapses has been suggested. A part of the synaptic contacts of the asymmetric and symmetric types is supposed to be genetically programmed and membrane specialization of these contacts is formed earlier than synaptic vesicles appear. Other part of the synapses undergoes certain stages of differentiation before the functionally mature contact is formed. The initial stage in the synapses formation in formation of the desmosome-like junction. The second stage is appearance of synaptic vesicles in the area of this contact. The third stage includes development of pre- and postsynaptic membranous specialization and owing to this the contact acquires either asymmetric or symmetric appearance. For the ontogenetic periods investigated establishment of complex forms of the intercellular junctions (tangent, reciprocal, etc.) is specific; this evidently demonstrates certain plastic rearrangements in the synapses during the process of development.

[Various types of non-synaptic intercellular contacts in the developing brain of the rat]. / Razlichnye vidy nesinapticheskikh mezhkletochnykh kontaktov v razvivaiushchemsia mozge krysy.

Peculiarities of ultrastructural organization and localization of early forms of avascular nonsynaptic types of junctions formed in 14-18-day-old rat embryos have been studied; cerebral structures different in their phylogenic relations (the sensomotor cortex and nucleus caudatus) are taken as an example. Five main types of nonsynaptic intercellular junctions have been revealed: desmosome-like, gap, symmetric, asymmetric and mixed junctions. They differ by their ultrastructural organization. These types of junctions make the main types of contacts: soma-somatic, dendro-somatic, dendro-dendritic, axo-somatic, axo-dendritic. Desmosomes form the greatest number of the contacts. The earliest and the most primitive are gap junctions; they, evidently, reflect functional activity of desmosome-like junctions. The mixed junctions, perhaps, reflect the developmental stages of the intercellular contacts of transition from one type of junctions into another. Localization peculiarities of the nonsynaptic intercellular contacts are demonstrated: glomerule-like formations, establishment of numerous contacts looking like a successive chain, and so on. For some other indices a longer period of intercellular contact formation in the nucleus caudatus is noted, comparing the sensomotor cortex, though the latter is a newer structural cerebral formation from the phylogenic point of view.