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Biochem Int ; 27(5): 811-22, 1992 Aug.
Article in English | MEDLINE | ID: mdl-1329751

ABSTRACT

Mouse monoclonal antibodies to the bovine brain regulatory subunit type II (RII) were produced and antibodies of 11 clones were characterized. We determined their subclass, affinity constants, competivity and influence on two RII functions, namely cAMP binding and inhibition of the catalytic subunit (C) activity. 4 clones produced antibodies that increased RII-cAMP binding 1,5-2-fold. Antibodies of other 3 clones prevented R-C association and inhibition of C phosphotransferase activity. Some antibodies affected neither of these RII functions.


Subject(s)
Antibodies, Monoclonal/immunology , Brain/enzymology , Cyclic AMP/metabolism , Protein Kinases/immunology , Animals , Antibodies, Monoclonal/biosynthesis , Antibodies, Monoclonal/isolation & purification , Antibody Specificity/immunology , Ascitic Fluid/metabolism , Cattle , Cyanogen Bromide , Enzyme-Linked Immunosorbent Assay , Hybridomas/immunology , Hydrolysis , Phosphotransferases/metabolism , Protein Binding
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