ABSTRACT
The paper is concerned with the results of experimental investigations to develop methods for obtaining 99mTc and 67Ga-labeled liposomes, possessing tumor-tropic properties. Sphingomyelin vesiculae, labeled with 67Ga with the help of bifunctional chelates, were shown to possess a high level of hyperfixation. A study of biodistribution of these liposomes in rats with Plis lymphosarcoma showed that the level of accumulation of 67Ga-liposomes was enough to obtain a clear scintigraphic tumor image.
Subject(s)
Lymphoma, Non-Hodgkin/diagnostic imaging , Sarcoma, Experimental/diagnostic imaging , Animals , Gallium Radioisotopes/administration & dosage , Liposomes , Male , Radionuclide Imaging , Rats , Technetium/administration & dosageABSTRACT
A study was made of the distribution of 125I (a chloramine method of labeling) monoclonal antibodies to the surface antigen Ly 2.1 of T-lymphocytes during action of immunomodulators (tactivin, hydrocortisone, tactivin administered after hydrocortisone) on ACR mice. These antibodies were shown to retain antigen binding capacity, permitting monitoring of the redistribution of the antigen in the body exposed to immunomodulators.
Subject(s)
Adjuvants, Immunologic/pharmacology , Antibodies, Monoclonal/pharmacokinetics , Antigens, Ly/immunology , Iodine Radioisotopes , T-Lymphocytes/immunology , Animals , Female , Hydrocortisone/pharmacology , Male , Mice , Mice, Inbred AKR , Peptides/pharmacology , T-Lymphocytes/drug effects , Thymus Extracts/pharmacology , Time Factors , Tissue Distribution/drug effectsABSTRACT
A study was made of the biodistribution of 125I-labeled (chloramine, lactoperoxidase and iodogen tracer methods) monoclonal antibodies to free lambda and kappa chains of human immunoglobulins (Bence Jones proteins) using experimental models: ACR mice with a depot of antigen in the muscular tissue. Labeled MAB were shown to preserve their immunological properties. The results of investigation into biodistribution helped to reveal selective accumulation of radioactivity in the antigen depot (Bence Jones protein).
Subject(s)
Antibodies, Monoclonal/pharmacokinetics , Bence Jones Protein/immunology , Iodine Radioisotopes/pharmacokinetics , Animals , Antibodies, Monoclonal/isolation & purification , Chromatography, Gel , Enzyme-Linked Immunosorbent Assay , Isotope Labeling , Mice , Mice, Inbred AKR , Time Factors , Tissue DistributionABSTRACT
The investigation of 22 patients has established the concentration of fluothane in the blood to influence the catecholamine content and the cardio-vascular system. Its increased concentration in the blood results in certain decrease of the level of general catecholamines in the blood, decreased value of systolic arterial pressure and increased frequency of cardiac contractions.
