Mercury accumulation and biomarkers of exposure in two popular recreational fishes in Hawaiian waters.

Mercury (Hg) exposure has not been examined in many recreational nearshore fish species that are commonly consumed around the Hawaiian Islands. Specific gene transcripts, such as metallothionein (MET) and thioredoxin reductase (TrxR), can be used to examine Hg exposure responses in aquatic organisms. This study measured total mercury (THg) in four species from two groups of Hawaiian nearshore fishes: giant trevally (Caranx ignobilis, n = 13), bluefin trevally (C. melampygus, n = 4), sharp jaw bonefish (Albula virgata, n = 2), and round jaw bonefish (A. glossodonta, n = 19). Total Hg accumulation and abundance profiles of MET and TrxR were evaluated for muscle, liver, and kidney tissues. Total Hg in round jaw bonefish and giant trevally tissues accumulated with length and calculated age. In round jaw bonefish tissues, mean THg was greater in kidney (1156 ng/g wet mass (wm)) than liver (339 ng/g wm) and muscle (330 ng/g wm). Giant trevally muscle (187 ng/g wm) and liver (277 ng/g wm) mean THg did not differ significantly. Fish species in this study were compared to commercial and local fish species with state and federal muscle tissue consumption advisories based on THg benchmarks developed by the U.S. Food and Drug Administration (FDA) and Environmental Protection Agency (EPA). Both bonefishes had mean muscle THg that exceeded benchmarks suggesting consumption advisories should be considered. MET transcript in round jaw bonefish kidney tissue and kidney THg exhibited a marginally significant positive correlation, while TrxR transcript in liver tissue negatively correlated with increasing liver THg. These results contribute to our understanding of Hg exposure associated health effects in fish.

Fish swimming mode and body morphology affect the energetics of swimming in a wave-surge water flow.

Fish swimming modes and the shape of both the fins and body are expected to affect their swimming ability under different flow conditions. These swimming strategies and body morphologies often correspond to distributional patterns of distinct functional groups exposed to natural and variable water flows. In this study, we used a swimming-respirometer to measure energetic costs during prolonged, steady swimming and while station holding in a range of simulated oscillatory wave-surge water flows, within the natural range of flow speeds and wave frequencies on coral reefs. We quantified the net cost of swimming (NCOS, metabolic costs above resting) for four reef fish species with differences in swimming mode and morphologies of the fin and body: a body and caudal fin (BCF) swimmer, the Hawaiian flagtail, Kuhlia xenura, and three pectoral fin swimmers, the kole tang, Ctenochaetus strigosus, the saddle wrasse, Thalassoma duperrey, and the Indo-Pacific sergeant major, Abudefduf vaigiensis. We found that the BCF swimmer had the highest rates of increase in NCOS with increasing wave frequency (i.e. increased turning frequency) compared with the pectoral fin swimmers. The wrasse, with a more streamlined, higher body fineness, had lower rates of increase in NCOS with increasing swimming speeds than the low body fineness species, but overall had the highest swimming NCOS, which may be a result of a higher aerobic swimming capacity. The deep-bodied (low fineness) pectoral fin swimmers (A. vaigiensis and C. strigosus) were the most efficient at station holding in oscillating, wave-surge water flows.

Bidirectional cyclical flows increase energetic costs of station holding for a labriform swimming fish, Cymatogaster aggregata.

Wave-induced surge conditions are found in shallow marine ecosystems worldwide; yet, few studies have quantified how cyclical surges may affect free swimming animals. Here, we used a recently adapted respirometry technique to compare the energetic costs of a temperate fish species (Cymatogaster aggregata) swimming against a steady flow versus cyclical unidirectional and bidirectional surges in which unsteady swimming (such as accelerating, decelerating and turning) occurs. Using oxygen uptake (MO2) as an estimate of energetic costs, our results reveal that fish swimming in an unsteady (i.e. cyclical) unidirectional flow showed no clear increase in costs when compared to a steady flow of the same average speed, suggesting that costs and savings from cyclical acceleration and coasting are near equal. Conversely, swimming in a bidirectional cyclical flow incurred significantly higher energetic costs relative to a steady, constant flow, likely due to the added cost of turning around to face the changing flow direction. On average, we observed a 50% increase in MO2 of fish station holding within the bidirectional flow (227.8 mg O2 kg-1 h-1) compared to a steady, constant flow (136.1 mg O2 kg-1 h-1) of the same mean velocity. Given wave-driven surge zones are prime fish habitats in the wild, we suggest the additional costs fish incur by station holding in a bidirectional cyclical flow must be offset by favourable conditions for foraging and reproduction. With current and future increases in abiotic stressors associated with climate change, we highlight the importance of incorporating additional costs associated with swimming in cyclical water flow in the construction of energy budgets for species living in dynamic, coastal habitats.

Swimming in unsteady water flows: is turning in a changing flow an energetically expensive endeavor for fish?

Unsteady, dynamic flow regimes commonly found in shallow marine ecosystems such as coral reefs pose an energetic challenge for mobile organisms that typically depend on station-holding for fitness-related activities. The majority of experimental studies, however, have measured energetic costs of locomotion at steady speeds, with only a few studies measuring the effects of oscillatory flows. In this study, we used a bidirectional swimming respirometer to create six oscillatory water flow regimes consisting of three frequency and amplitude combinations for both unidirectional and bidirectional oscillatory flows. Using the goldring surgeonfish, Ctenochaetus strigosus, a pectoral-fin (labriform) swimmer, we quantified the net cost of swimming (swimming metabolic rate minus standard metabolic rate) associated with station-holding under these various conditions. We determined that the swimming costs of station-holding in the bidirectional flow regime increased by 2-fold compared with costs based on swimming over the same range of speeds at steady velocities. Furthermore, as we found minimal differences in energetic costs associated with station-holding in the unidirectional, oscillating flow compared with that predicted from steady swimming costs, we conclude that the added acceleration costs are minimal, while the act of turning is an energetically expensive endeavor for this reef fish species.

Swimming in unsteady water flows: is turning in a changing flow an energetically expensive endeavor for fish?

Unsteady, dynamic flow regimes commonly found in shallow marine ecosystems such as coral reefs pose an energetic challenge for mobile organisms that typically depend on station holding for fitness-related activities. The majority of experimental studies, however, have measured energetic costs of locomotion at steady speeds, with only a few studies measuring the effects of oscillatory flows. In this study, we used a bidirectional swimming respirometer to create six oscillatory water flow regimes consisting of three frequency and amplitude combinations for both unidirectional and bidirectional oscillatory flows. Using the goldring surgeonfish, Ctenochaetus strigosus, a pectoral-fin (labriform) swimmer, we quantified the net cost of swimming (swimming metabolic rate minus standard metabolic rate) associated with station-holding under these various conditions. We determined that the swimming costs of station-holding in the bidirectional flow regime increased by 2-fold compared with costs based on swimming over the same range velocities at steady speeds. Furthermore, as we found minimal differences in energetic costs associated with station-holding in the unidirectional, oscillating-flow compared with that predicted from steady swimming costs, we conclude that the added acceleration costs are minimal, while the act of turning is an energetically expensive endeavor for this reef fish species.

Energetics and behavior of coral reef fishes during oscillatory swimming in a simulated wave surge.

Oxygen consumption rates were measured for coral reef fishes during swimming in a bidirectional, oscillatory pattern to simulate station-holding in wave-induced, shallow-water flows. For all species examined, increases in wave intensity, as simulated by increases in frequency and amplitude of oscillation, yielded increased metabolic rates and net costs of swimming (NCOS; swimming metabolic rate minus standard metabolic rate). Comparing species with different swimming modes, the caudal fin swimming Kuhlia spp. (Kuhliidae) and simultaneous pectoral-caudal fin swimming Amphiprion ocellaris (Pomacentridae) turned around to face the direction of swimming most of the time, whereas the median-paired fin (MPF) swimmers, the pectoral fin swimming Ctenochaetus strigosus (Acanthuridae) and dorsal-anal fin swimming Sufflamen bursa (Balistidae), more frequently swam in reverse for one half of the oscillation to avoid turning. Contrary to expectations, the body-caudal fin (BCF) swimming Kuhlia spp. had the lowest overall NCOS in the oscillatory swimming regime compared with the MPF swimmers. However, when examining the effect of increasing frequency of oscillation at similar average velocities, Kuhlia spp. showed a 24% increase in NCOS with a 50% increase in direction changes and accelerations. The two strict MPF swimmers had lower increases on average, suggestive of reduced added costs with increasing frequency of direction changes with this swimming mode. Further studies are needed on the costs of unsteady swimming to determine whether these differences can explain the observed prevalence of fishes using the MPF pectoral fin swimming mode in reef habitats exposed to high, wave-surge-induced water flows.

Effects of salinity on metabolic rate and branchial expression of genes involved in ion transport and metabolism in Mozambique tilapia (Oreochromis mossambicus).

This study investigated the effects of two rearing salinities, and acute salinity transfer, on the energetic costs of osmoregulation and the expression of metabolic and osmoregulatory genes in the gill of Mozambique tilapia. Using automated, intermittent-flow respirometry, measured standard metabolic rates (SMRs) of tilapia reared in seawater (SW, 130 mg O2 kg⁻¹ h⁻¹) were greater than those reared in fresh water (FW, 103 mg O2 kg⁻¹ h⁻¹), when normalized to a common mass of 0.05 kg and at 25±1°C. Transfer from FW to 75% SW increased SMR within 18h, to levels similar to SW-reared fish, while transfer from SW to FW decreased SMR to levels similar to FW-reared fish. Branchial gene expression of Na⁺-K⁺-2Cl⁻ cotransporter (NKCC), an indicator of SW-type mitochondria-rich (MR) cells, was positively correlated with SMR, while Na⁺-Cl⁻ cotransporter (NCC), an indicator of FW-type MR cells, was negatively correlated. Principal Components Analysis also revealed that branchial expression of cytochrome c oxidase subunit IV (COX-IV), glycogen phosphorylase (GP), and a putative mitochondrial biogenesis regulator in fish, peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), were correlated with a higher SMR, plasma osmolality, and environmental salinity, while expression of glycogen synthase (GS), PGC-1ß, and nuclear respiratory factor 1 (NRF-1) had negative correlations. These results suggest that the energetic costs of osmoregulation are higher in SW than in FW, which may be related to the salinity-dependent differences in osmoregulatory mechanisms found in the gills of Mozambique tilapia.

Energetics of median and paired fin swimming, body and caudal fin swimming, and gait transition in parrotfish (Scarus schlegeli) and triggerfish (Rhinecanthus aculeatus).

To determine the energetic costs of rigid-body, median or paired-fin (MPF) swimming versus undulatory, body-caudal fin (BCF) swimming, we measured oxygen consumption as a function of swimming speed in two MPF swimming specialists, Schlegel's parrotfish and Picasso triggerfish. The parrotfish swam exclusively with the pectoral fins at prolonged swimming speeds up to 3.2 total lengths per second (L s(-1); 30 min critical swimming speed, U(crit)). At higher speeds, gait transferred to a burst-and-coast BCF swimming mode that resulted in rapid fatigue. The triggerfish swam using undulations of the soft dorsal and anal fins up to 1.5 L s(-1), beyond which BCF undulations were recruited intermittently. BCF swimming was used continuously above 3.5 L s(-1), and was accompanied by synchronous undulations of the dorsal and anal fins. The triggerfish were capable of high, prolonged swimming speeds of up to 4.1 L s(-1) (30 min U(crit)). In both species, the rates of increase in oxygen consumption with swimming speed were higher during BCF swimming than during rigid-body MPF swimming. Our results indicate that, for these species, undulatory swimming is energetically more costly than rigid-body swimming, and therefore support the hypothesis that MPF swimming is more efficient. In addition, use of the BCF gait at higher swimming speed increased the cost of transport in both species beyond that predicted for MPF swimming at the same speeds. This suggests that, unlike for terrestrial locomotion, gait transition in fishes does not occur to reduce energetic costs, but to increase recruitable muscle mass and propulsive surfaces. The appropriate use of the power and exponential functions to model swimming energetics is also discussed.