ABSTRACT
Metabolites of 3-[2-(N,N-dimethylaminoethyl)phenylthio]phenol (I) were isolated from the urine of rats, mice, rabbit and dog and from the faeces of rats by extraction and thin-layer chromatography. From the mass spectrum of I and using characteristics mass shifts in the spectra of metabolites, the structures of four metabolites were determined, whereas for two other isomeric metabolites the structures were resolved by means of IR spectra. Conclusions regarding the structures derived from the spectra were confirmed by comparing them with synthetic standards. Generally, metabolic changes of I can be characterized by demethylation, hydroxylation combined with methylation, S-oxidation and by a combination of these metabolic reactions.
Subject(s)
Benzylamines/analysis , Serotonin Antagonists/metabolism , Animals , Benzylamines/metabolism , Benzylamines/urine , Chromatography, Thin Layer , Dogs , Feces/chemistry , Male , Mass Spectrometry , Mice , Rabbits , Rats , Rats, Inbred Strains , Serotonin Antagonists/urine , Spectrophotometry, InfraredABSTRACT
Metabolites of drug VUFB 15468 present in the urine of rats, mice and dogs and in rat faeces after peroral administration were determined qualitatively. The relative representation of the individual metabolites in the urine of rats and mice was determined radiometrically after p. o. administration of [3H]VUFB 15468. For qualitative demonstration the metabolites were extracted; they were detected, partially identified and purified by thin-layer chromatography and then analyzed by mass spectrometry and IR-spectrometry. Structures of 11 metabolites were completely or partially determined in rat urine. Five of them were also found in rat faeces, one in murine urine and five in canine urine. In all animal species also unchanged VUFB 15468 was found. For quantification of the individual metabolites and VUFB 15468, TLC-radiometry and liquid scintillation spectrometry were used in rats and mice. Of the relative representation of metabolites, about one third is unchanged VUFB 15468, the rest are metabolites. In mice, the proportion of unchanged VUFB 15468 is much higher, about two thirds.
Subject(s)
Antidepressive Agents/metabolism , Benzylamines/metabolism , Animals , Biotransformation , Dogs , Male , Mice , Mice, Inbred Strains , Rats , Rats, Inbred StrainsABSTRACT
The cellular fatty acid composition determined by gas chromatography-mass spectrometry, was found not to differ among Listeria monocytogenes, L. innocua and L. ivanovii. Slight quantitative differences found in the fatty acid pattern of L. welshimeri were significantly pronounced in L. denitrificans. L. murrayi and L. grayi displayed characteristic closely related patterns. Considerable amounts of fatty aldehydes and their dimethyl acetals were observed in hydrolysates and methanolysates of L. seeligeri. The fatty acid composition of Erysipelothrix rhusiopathiae was found to strongly differ from that of Listeria.
Subject(s)
Aldehydes/analysis , Erysipelothrix/analysis , Fatty Acids/analysis , Listeria/analysis , Erysipelothrix/classification , Gas Chromatography-Mass Spectrometry , Listeria/classificationSubject(s)
Bromosuccinimide , Dibenzothiepins , Succinimides , Chemical Phenomena , Chemistry , Drug Interactions , Humans , Spectrometry, FluorescenceSubject(s)
Antineoplastic Agents/metabolism , Fluorenes/metabolism , Animals , Humans , Mice , Rats , Swine , Swine, MiniatureSubject(s)
Antineoplastic Agents/blood , Fluorenes/blood , Mass Spectrometry/methods , Humans , KineticsSubject(s)
Antineoplastic Agents/blood , Fluorenes/blood , Mass Spectrometry/methods , Animals , Deuterium , SwineABSTRACT
Metabolites of benzofenac (3-chloro-4- benzyloxyphenylacetic acid) were determined in rat, rabbit, dog, and human urine by GC/MS. Before analysis the urine was extracted and the extracts were treated with diazomethane. Deuterium-labelled benzofenac , 3-chloro-4-[( methylene-2H2]- benzolyoxy ) phenylacetic acid was used for the elucidation of the structures of some main metabolites and the determination of trace metabolites. The structures of the metabolites were determined from spectra obtained by electron impact and chemical ionization.
