ABSTRACT
We performed a seroepidemiological survey of the level and structure of population immunity to SARS-CoV-2 among employees of medical institution in the Irkutsk region during the COVID-19 pandemic. Seroprevalence assessment was organized from May 2020 to April 2021. The level of antibodies to SARS-CoV-2 was measured by ELISA. It was found that 139 (46%) of 299 examined workers were seropositive, including 50 (36%) vaccinated against COVID-19, 75 (54%) patients diagnosed with COVID-19, and 14 (10%) asymptomatic cases of SARS-CoV-2. The results obtained should be taken into account when predicting the dynamics of the epidemic process and organizing preventive (antiepidemic) measures, including vaccination.
Subject(s)
COVID-19 , Antibodies, Viral , COVID-19/epidemiology , COVID-19/prevention & control , Humans , Immunity, Humoral , Pandemics/prevention & control , SARS-CoV-2 , Seroepidemiologic StudiesABSTRACT
We studied the expression of activation markers CD25 and CD69 by blood lymphocytes in white mice vaccinated with Brucella abortus 19 BA in antigen-specific tests in vitro. During incubation of blood lymphocytes with brucellosis polysaccharide-protein antigen, a statistically significant increase in the expression of CD25 by B cells and CD69 by T cells was observed; brucellin increased the expression of CD25 by B and T cells. Comparative analysis of the action of antigen preparations B. abortus showed that only brucellin has antigen-specific activity against CD19+CD25+ cells. The used method can be considered as a promising test for evaluation of the effectiveness of brucellosis immunoprophylaxis, which substantiates the need for further research.
Subject(s)
Antigens, Bacterial/administration & dosage , B-Lymphocytes/drug effects , Brucella abortus/immunology , Brucellosis/immunology , T-Lymphocytes/drug effects , Animals , Antigens, Bacterial/immunology , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Differentiation, T-Lymphocyte/genetics , Antigens, Differentiation, T-Lymphocyte/immunology , B-Lymphocytes/immunology , B-Lymphocytes/microbiology , Brucellosis/diagnosis , Brucellosis/microbiology , Female , Flow Cytometry , Gene Expression , Immunophenotyping , Interleukin-2 Receptor alpha Subunit/genetics , Interleukin-2 Receptor alpha Subunit/immunology , Lectins, C-Type/genetics , Lectins, C-Type/immunology , Lymphocyte Activation/drug effects , Mice , T-Lymphocytes/immunology , T-Lymphocytes/microbiology , VaccinationABSTRACT
The effects of nanostructurized composites argentogalactomannan and argento-poly-1-vinyl-1,2,4-triasole on the kinetics of T cell maturation in the thymus of albino mice were studied. These nanocomposites stimulated the formation of CD3+CD4+CD8- and CD3+CD4-CD8+ cells from CD3-CD4-CD8- cells, the effect of argentogalactomannan was more potent. The efficiency of these compounds as agents improving the defense potential deserves special studies.
Subject(s)
Metal Nanoparticles/adverse effects , Nanocomposites/adverse effects , Silver/adverse effects , Thymus Gland/pathology , Lymphocyte Count , T-Lymphocyte Subsets/drug effects , Thymus Gland/drug effects , Vaccines/adverse effectsABSTRACT
Morphological changes in the immunocompetent organs of white mice with experimental plague infection manifested in activation of the immune response of different degree and pathological process of different severity that depended on the plasmid composition of Y. pestis. Widening of the T-dependent zones in the immune organs of white mice infected with isogenic strains of Y. pestis with different plasmid composition attests to activation of cellular immunity. Our findings allow considering Y. pestis subsp. altaica I-2948/3, Y. pestis subsp. pestis I-3479 and Y. pestis subsp. pestis I-3480 as promising candidates for vaccine strains.
