[The influence of the fluorine-containing activators of mitochondrial adenosine triphosphate sensitive potassium channels on the oxidative phosphorilation].

The cardioprotective mechanism of KATP channel openers and especially their influence on mitochondrial respiration has not been clarified yet. In this article we investigated the effect of DiazoFm and DiazoFp, the new fluor-containing analogues of diazoxide and the potential mitochondrial KATP channel openers, on the oxidative phosphorylation in the isolated mitochondria. It was shown that the influence of KATP channel openers on ADP-stimulated oxygen consumption (State 3) depended on the substrates we used (succinate or 2-oxoglutarate sodium). We have shown that the depression of State 3 was less when we used DiazoFm (30 MM) and DiazoFp (30 MM) in comparison with Diazoxide in experiments where succinate was used. The fluor-containing KATP, channels openers did not significantly change the activity of succinate dehydrogenase in comparison with diazoxide (it decreased succinate dehydrogenase activity by 27%). Thus, the fluor-containing analogues of diazoxide did not significant influence on the complex II of the respiratory chain. In the other experiments when we used 2-oxoglutarate sodium as an oxidative substrate, DiazoFp increased ADP-stimulated oxygen consumption by 33%. All the studied KATP openers have an uncoupling effect, regardless the substrates we used. This effect was more significant when we used succinate as a substrate. We have shown that the uncoupling effect of oxidative phosphorylation is a consequence of K channels activation. This statement was proved by 5-hydroxydecanoate (200 MM) with depressed influence of Diazoxide and its fluoring-containing analogues. Conclusion. The fluor-containig KATP channels openers had not direct influence on the respiratory chain in mitochondria, but activation mitochondrial KATP channels by them lead to uncoupling phosporylation and respiration.

[Effect of omega-3 polyunsaturated fatty acids on activity of glutathione-dependent enzymes in the liver cytosol and blood erythrocytes in rats with experimental chronic bronchitis and in the norm]. / Vliianie omega-3 polinenasyshchennykh zhirnykh kislot na aktivnost' glutationzavisimykh fermentov v tsitozole pecheni i éritrotsitakh krovi krys v norme i pri éksperimental'nom khronicheskom bronkhite.

The influence of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on the activity of glutathione reductase, glutathione transferase and glutathione peroxidase in the liver cytosole and red blood cells of normal rats and animals with experimental chronic bronchitis. omega-3 PUFA ("Tekom" medication) activate glutathione reductase of liver cytosole and glutathionperoxidase in the red blood cells in rats. In the rats with chronic inflammatory process in bronchia omega-3 PUFA corrects the glutathione-dependent systems of detoxication. Effects were more expressed in the liver cytosole in comparison with the red blood cells. The using of omega-3 PUFA as a means for treatment and prophylaxis was more effective than for treatment only.

[The antitryptic activity of the blood serum in patients with chronic bronchitis and lung cancer in relation to the phenotypic variant of the alpha-1 proteinase inhibitor]. / Antitripticheskaia aktivnost' syvorotki krovi u bol'nykh khronicheskim bronkhitom i rakom legkikh v zavisimosti ot fenotipicheskogo varianta al'fa-1-ingibitora proteinaz.

Blood serum was studied for its antitryptic activity in 161 patients with chronic bronchitis, 87 individuals with malignant tumors of the respiratory organs, 70 blood donors, with regard to phenotypic variant of alpha 1-inhibitor of proteinases. Difference was revealed in antitryptic activity, associated with the particular features of the pathologic process and phenotypic variant of the inhibitor. There exists in the carriers of homozygote latent insufficiency of the inhibitory defence of the organism against the proteolytic effects.

[State of microsomal oxidation enzymes as a function of acetyltransferase activity]. / Sostoianie sistemy fermentov mikrosomal'nogo okisleniia v zavisimosti ot atsetiltransferaznoi aktivnosti.

Quantity and activity of components in the monoxygenase system of the liver microsomes have been studied for their dependence on the phenotype of acetylation. Results of investigations are presented. The majority of these enzymes exist as numerous molecular forms: isozymes. It is shown that activity of aminopyrine-N-demethylase and NADPH-cytochrome P-450 reductase depends on the phenotype of acetyltransferase.

[Effects of low-energy laser irradiation on blood biochemical parameters in experimental chronic obstructive bronchitis]. / Vozdeistvie nizkoénergeticheskogo lazernogo izlucheniia na biokhimicheskie pokazateli krovi pri éksperimental'nom khronicheskom obstruktivnom bronkhite.

The study established that shifts in oxidant-antioxidant system induced by chronic obstructive bronchitis are correctable by He-Ne laser irradiation. This conclusion was based on biochemical indices of the blood measured in experimental animals: lipid peroxidation, general antitryptic activity of the serum, activity of the enzymes superoxide dismutase, gamma-glutamyl transferase, pseudocholine esterase, alpha-oxybutyrate dehydrogenase, glucose 6-phosphate dehydrogenase.

[The results of treating pulmonary tuberculosis patients with different concomitant diseases by using millimeter-wave resonance therapy]. / Rezul'taty lecheniia bol'nykh tuberkulezom legkikh s razlichnymi soputstvuiushchimi zabolevaniiami pri primenenii millimetrovovolnovoi rezonansnoi terapii.

The authors investigated the clinical and biochemical efficacy of millimeter-wave resonance therapy in patients with destructive pulmonary tuberculosis associated with different concomitant diseases. It was established that inclusion of millimeter-wave resonance therapy in the complex treatment of this patient category favoured the efficacy of tuberculosis treatment. The treatment produced also a favourable effect on concomitant diseases.

[Glucose-6-phosphate dehydrogenase activity in patients with chronic inflammatory lung diseases]. / Aktivnost' gliukozo-6-fosfatdegidrogenazy u bol'nykh khronicheskimi vospalitel'nymi zabolevaniiami legkikh.

The activity of the key enzyme of the pentoso-phosphate cycle--glucoso-6-phosphatedehydrogenase was examined in patients with chronic inflammatory pulmonary diseases. Results indicate that with exacerbation of the process in the bronchopulmonary system there occurs an increase of the activity of the mentioned enzyme. Examination of the activity of this enzyme may be of value for optimization of treatment measures in patients with inflammatory pulmonary diseases.

[Effect of tubaside and sodium succinate on oxidative phosphorylation and p-hydroxylation in the liver]. / Vliianie tubazida i suktsinata natriia na okislitel'noe fosforilirovanie i p-gidroksilirovanie v pecheni.

Tubaside causes disturbances in oxidative phosphorylation of the liver mitochondria. Sodium succinate administered during tubaside intoxication favours normalization of the oxygen uptake rate in state 3 and the ADP phosphorylation rate. Sodium succinate weakens the toxic effect of tubaside and favours normalization of p-hydroxylation in the liver. The animals, which were given tubaside in combination with sodium succinate are characterized by a higher survival rate.

[Effect of ethionamide and sodium succinate on NADPH-dependent oxidaton in the liver]. / Vliianie zhtionamida i suktsinata natriia na (NADPH) zavisimoe okislenie v pechni.

A single administration of ethionamide (LD50) to guinea pigs causes an inhibition of p-hydroxylation and N-demethylation in the liver. Sodium succinate favours the activation and, when it is used for correction of the ethionamide toxic effect, it normalizes the above-mentioned processes.

[Oxidative phoshporylation, proton exchange and volume changes in hepatocyte mitochondria under the effect of exogenous succinate]. / Okislitel'noe fosforilirovanie, obmen protonov i ob'emnye izmeneniia mitokhondrii gepatotsitov pri vozdeistvii ekzogennogo suktsinata.

Long use of exogenous succinate causes changes in hepatocytes bioenergetic functions in guinea pigs. The character of cation is of essential significance in this situation: sodium succinate produces a moderate stimulating effect, potassium succinate evokes a distinct energizing influence.

[Effect of benemycin and sodium succinate on metabolism of protons in mitochondria of guinea pig lung and liver tissues]. / Vliianie benemitsina i suktsinata natriia na obmen protonov v mitokhondriiakh tkanei legkikh i pecheni morskikh svinok.

The data of studies on proton metabolism in mitochondria of the guinea pig lungs and liver with application of benemycin and sodium succinate show that benemycin does not affect the metabolism of protons in the lungs and liver mitochondria. Sodium succinate activates the release of protons from the lungs and liver mitochondria in state 4 according to Chance. When using sodium succinate for reducing the effect of benemycin on theorganism it is found that sodium succinate inhibits the utilization of protons of the lungs and liver mitochondria in state 3 according to Chance and activates this release in state 4, thus favouring an increase in their concentration in the extra-mitochondrial space. Sodium succinate increases survival rate of animals which were administered benemycin to.