ABSTRACT
Cells from different human wounds were analyzed concerning their degree of ploidy. The experiments showed an increased tetraploidization rate in well-healing wounds especially during inflammation and proliferation. Recent data described a polyploidization in different tissues, which is accompanied and maybe caused by the multiplication of the centrosome. We show here for the first time that cells from nonmalignant tissue, namely human wound cells, are characterized by an extensive centrosome multiplication. In an effort to identify a certain mechanism, by which the centrosome may act as a modulator of the cells' ploidy, we focused our interest on p53, whose interaction with the centrosome was recently described. Applying a wound model onto p53-wildtype (wt) and p53-knockout (ko) mice, we could show that polyploidization was not reversible in p53-ko mice during wound healing. The lack of p53, the centrosome multiplication, and the polyploidization therefore may contribute to the physiological process of tissue repair in physiologically "normal" tissue.
Subject(s)
Centrosome/ultrastructure , Polyploidy , Wound Healing/genetics , Animals , Genes, p53 , Humans , In Situ Hybridization, Fluorescence , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Wounds and Injuries/genetics , Wounds and Injuries/pathologyABSTRACT
To investigate the role of chromosomal alterations in the process of wound healing on the cellular level, we analyzed biopsies from well-healing (10) and chronic defect (8) wounds. Classical chromosome preparation and fluorescence in situ hybridization were performed with cultured cells and smear preparations. Results from both techniques showed an unusual high rate of tetraploid cells (4 n) in granulation tissue of well-healing wounds (6.5-60%), whereas we found only a low amount of tetraploid cells (from 0 to 5.5%) in chronic wounds. In fibroblast control cultures, there was a percentage of 2-5.5%. In chromosome preparations, we noticed an increased number of nonclonal structural and numerical chromosome aberrations in both well-healing and chronic wounds. Our data show clearly that especially tetraploidization is a typical phenomenon in the well-healing wound, where it apparently supports the healing process.
Subject(s)
Polyploidy , Wound Healing/genetics , Wound Healing/physiology , Cells, Cultured , Chromosome Aberrations , Chromosomes, Human, Pair 7 , Chromosomes, Human, Pair 8 , Female , Granulation Tissue/ultrastructure , Humans , In Situ Hybridization, Fluorescence , Karyotyping , MaleABSTRACT
Granulation tissue of normal and non-healing human wounds showed a similar distribution pattern of the cell populations investigated by FACS analysis, whereas only non-healing wounds revealed a reduced density of cells and intercellular matrix. Thus, supporting the thesis that impaired wound healing is not caused by changes of the cellular distribution pattern of granulation tissue, but possibly by lessened cell function.
Subject(s)
Flow Cytometry , Granulation Tissue/pathology , Wound Healing/physiology , Cell Count , Extracellular Matrix/pathology , HumansABSTRACT
In a defect wound model in rats local application of hyaluronan improves proliferation of granulation tissue and epithelialization under diabetic conditions, thus, supporting its use in therapy of problem wounds in diabetes.
