[Subcellular distribution of adenosine system enzymes in the hypothalamus and hippocampus of the rat brain]. / Subkletochnoe raspredelenie nekotornykh fermentov sistemy adenozina v gipotalamuse i gippokampe golovnogo mozga krys.

The subcellular distribution of 5'-nucleotidase and adenosine deaminase in rat brain hypothalamus and hippocampus was studied. In the hippocampus the 5'-nucleotidase activity was shown to be much higher than in the hypothalamus, while the adenosine deaminase activity, contrariwise, is nearly two times as high as that in the hypothalamus. During the analysis of subcellular distribution 5'-nucleotidase and adenosine deaminase were detected in all fractions under study, i. e., in nuclear, soluble, myelin fractions as well as in synaptic membranes, synaptosomes and "pure" mitochondria. The highest 5'-nucleotidase activity was found in the myelinic and synaptic fractions both in the hypothalamus and in the hippocampus. The highest adenosine deaminase activity was detected in the soluble fraction of the above structures. The enzyme activity in synaptic membranes and synaptosomes was nearly two times as low.

[Effects of desoxycorticosterone on 5-nucleotidase and adenosine deaminase activity in the hypothalamus and hippocampus of the rat brain]. / Vliianie dezoksikortikosterona na aktivnost' 5'-nukleotidazy i adenozindezaminazy v gipotalamuse i gippokampe golovnogo mozga krys.

Influence of single (enzyme activities was determined 5h after the preparation) and multiple (for 7 days) DOCA administration on 5'-nucleotidase (EC 3.1.3.5) and adenosine deaminase (ES 3.5.4.4) activities in homogenate and subcellular fractions of the hypothalamus and hippocampus of the rat brain has been studied. It has been shown that DOCA enhances adenosine metabolism in the hypothalamus activating both the enzymes while in the hippocampus 5'-nucleotidase activity increases whereas adenosine deaminase activity decreases. Possible ways of mineralocorticoid effects on adenosine system and the role of this nucleoside in these hormones mechanism of action on CNS functional activity are being discussed.

[The effect of hydrocortisone on the activity of 5'-nucleotidase and adenosine deaminase in the hypothalamus and hippocampus of the rat brain]. / Vliianie gidrokortizona na aktivnost' 5'-nukleotidazy i adenozindezaminazy v gipotalamuse i gippokampe golovnogo mozga krys.

The effect of single (1.4 and 24 h) and long-term (7 days) hydrocortisone intramuscular administration (5 mg/100 g of body mass) on 5'-nucleotidase and adenosine deaminase activities in homogenate and subcellular fractions of the rat brain hypothalamus and hippocampus has been studied. Enzymatic activity of adenosine metabolism in hypothalamus and hippocampus has been shown to undergo changes with the duration of hydrocortisone administration. A possible role of adenosine in the mechanism of glucocorticoid action in the CNS and the regulation of the hypothalamic-pituitary-adrenal axis is under discussion.

[The effect of splenic humoral factors on corticosteroid metabolism and the cAMP content in the liver of rats with experimental toxic hepatitis]. / Vliianie gumoral'nykh faktorov selezenki na metabolizm kortikosteroidov i soderzhanie tsAMF v pecheni krys pri éksperimental'nom toksicheskom gepatite.

Corticosteroid metabolism in the liver and the level of c-AMP in the liver and spleen of rats with experimental toxic hepatitis were investigated after splenin administration. Hydrocortisone metabolites were isolated by a method of two-dimensional thin-layer chromatography. It was shown that their production and the level of c-AMP in the liver and spleen after simulation of experimental hepatitis were considerably reduced. The administration of this drug returned the indices under study to normal. The results obtained demonstrated that humoral factors could produce protective action in toxic liver lesion.

[Effect of corticotropin and hydrocortisone on the cyclic AMP content and phosphodiesterase activity in the skeletal and smooth muscles of rats]. / Vliianie kortikotropina i gidrokortizona na soderzhanie tsiklicheskogo AMF i aktivnost' fosfodiésterazy v skeletnykh i gladkikh myshtsakh krys.

A study was made of the influence of ACTH and hydrocortisone on cAMP content and phosphodiesterase (PDE) activity in the rat gastrocnemius and gastric skeletal muscles. One and 3 hours after injection hydrocortisone caused a decrease in PDE activity and raised cAMP content in both the muscles. ACTH in a dose of 1 Unit/100 g did not change PDE activity but increased cAMP content in the muscles one hour after injection, with an elevation in the blood corticosteroid level being insignificant. Three hours after injection of 2 Units/100 g ACTH, PDE activity dropped and muscle cAMP content ascended, with an appreciable increase in the blood corticosteroid level. In the smooth muscles, PDE activity was inhibited by corticosteroids to a greater degree than in the skeletal muscles.

[Effect of a varying corticosteroid allowance on the cAMP content of limbic system structures of the rat brain]. / Vliianie raznoi obespechennosti organizma kortikosteroidami na soderzhanie tsAMF v nekotorykh strukturakh limbicheskoi sistemy golovnogo mozga krys.

A study was made of the effect of adrenalectomy and substitution therapy in the absence of adrenals on the cAMP content in the hypothalamus, hippocamp and striate body of the rat brain. It was shown that in sham adrenalectomized animals, the concentration of cAMP in the brain structures under study did not differ from the characteristics recorded in the control group. Adrenalectomy in rats was followed by a significant reduction of the nucleotide in all the structures, namely in the hypothalamus by 89.5%, and in the hippocamp and striate body by 30.3 and 30.5%, respectively. Application of substitution therapy (5 mg hydrocortisone per 100 mg body mass) prevented the decreased content of cAMP seen in the adrenalectomized rats, with its magnitude being within the limits recorded in the intact animals. Doca was little effective in this aspect.

[Cyclic adenosine-3',5'-monophosphate content in the limbic system structures of the rat brain on the administration of corticosteroids]. / Soderzhanie tsiklicheskogo adenozin-3',5'-monofosfata v nekotorykh strukturakh limbicheskoi sistemy golovnogo mozga krys pri vvedenii kortikosteroidov.

The effect of hydrocortisone and DOCA on the cAMP content in the hypothalamus, hippocampus and striate body of the rat brain was investigated. Single (determined after 1 and 24 hours) and repeated (7 days) hydrocortison administration in a dose of 5 mg/100 g body weight was accompanied by an increase in the cAMP concentration in the brain structures under study. Single administration of DOCA in a dose of 0.5 mg/100 g body weight did not produce any changes in the cAMP level in the structures of the rat brain limbic system; however, the dose of 2.5 mg raised the cAMP level. Prolonged administration of the hormone in the above doses dod not change the cAMP level in the brain structures. Only the hippocampus showed a 210% increase in the cAMP level during DOCA administration in a dose of 0.5 mg.