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A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC.

Alimov, A A; Rodova, M A; Gizatullin, R Z; Kost-Alimova, M Y; Fedorova, L I; Barsky, V E; Kashuba, V; Klein, G; Zabarovsky, E R; Zelenin, A V.

Genet Anal ; 14(1): 21-3, 1997 Mar.

Article in English | MEDLINE | ID: mdl-9158962

ABSTRACT

We demonstrate that micro-dissection can be used for isolating NotI linking clones from the human 3p21-pter region. This approach is an improvement to positional cloning techniques, since NotI linking clones are directly linked with genes.

Subject(s)

Carcinoma, Renal Cell/genetics , Carcinoma, Small Cell/genetics , Cloning, Molecular/methods , Micromanipulation/methods , Base Sequence , Chromosome Deletion , Chromosome Mapping/methods , Chromosomes, Human, Pair 3 , Deoxyribonucleases, Type II Site-Specific , Humans , Molecular Sequence Data

ABSTRACT

Subject(s)

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