ABSTRACT
OBJECTIVE: To evaluate efficacy and safety of hypoxic-hyperoxic therapy in rehabilitation after SARS-CoV-2 pneumonia. MATERIAL AND METHODS: There were 44 patients aged 49-75 years in early recovery period after previous SARS-CoV-2 pneumonia (ICD-10 U07.1 and U07.2). Patients were divided into 2 groups depending on treatment strategy: group 1 (n=23) - rehabilitation treatment with therapeutic exercises, massage and physiotherapy; group 2 (n=21) - basic procedures with hypoxic-hyperoxic therapy. RESULTS: At initial evaluation, we found positive tendencies of rehabilitation in both groups. There were no side effects associated with hypoxic-hyperoxic therapy in the second group. Patients positively perceived this method of rehabilitation. There were significant between-group differences in exercise tolerance (p<0.05). These data indicate more favorable physical recovery in the second group and similar improvement of psycho-emotional status in both groups. CONCLUSION: Hypoxic-hyperoxic therapy is effective and safe approach in rehabilitation of patients with previous SARS-CoV-2 pneumonia. It is necessary to continue searching for effective methods of rehabilitation and develop accurate rehabilitation methods for various groups of patients.
Subject(s)
COVID-19 , SARS-CoV-2 , Exercise Therapy/methods , HumansABSTRACT
OBJECTIVE: To evaluate the effectiveness and safety of ozone therapy in rehabilitation of patients with previous COVID-19. MATERIAL AND METHODS: A randomized controlled clinical trial included 51 patients aged 29 - 78 years with SARS-CoV-2 pneumonia (J12.8). Patients were divided into 3 comparable groups depending on the complex of rehabilitation. In the first (control) group (n=17), a 10-day course included daily breathing exercises and physiotherapy for the lungs (drug electrophoresis and low-frequency magnetotherapy). In the second (main) group (n=18), rehabilitation was combined with daily intravenous infusions of ozonized saline with ozone concentration of 2.0 mg/l within 5 days with subsequent standard rehabilitation. In the third group (n=16), patients received 5 ozone therapy procedures every other day. To determine the effectiveness and safety of systemic ozone therapy in rehabilitation of patients with previous COVID-19, we analyzed oxygen saturation, laboratory data (D-dimer and C-reactive protein), need for oxygen support before and after rehabilitation course. Complaints and quality of life throughout the rehabilitation program were assessed using the EQ-5D questionnaire. RESULTS: All patients had positive changes of all parameters. There were no adverse reactions throughout the rehabilitation program and 2 months later. We observed higher effectiveness of rehabilitation with systemic ozone therapy. Moreover, daily ozone therapy had a better effect on laboratory parameters compared to ozone therapy every other day. CONCLUSION: Ozone therapy is safe and effective in complex rehabilitation of patients with previous COVID-19. Further studies of large samples are needed to determine indications and appropriate criteria for this rehabilitation program.
Subject(s)
COVID-19 , Ozone , Humans , Lung , Ozone/therapeutic use , Quality of Life , SARS-CoV-2 , Treatment OutcomeABSTRACT
OBJECTIVE: To analyze the efficacy and safety of therapeutic exercises and chest hardware massage in electrostatic field in patients with COVID-associated viral pneumonia. MATERIAL AND METHODS: We retrospectively analyzed 1551 patients admitted to the Clinical Hospital No. 1 (MEDSI Group JSC) with COVID-associated pneumonia between April 01, 2020 and June 15, 2021 (ICD-10 U07.1 and U07.2). Considering inclusion and exclusion criteria, we enrolled 153 patients. All patients were divided into comparable groups and subgroups depending on the methods of rehabilitation treatment and CT stage of viral pneumonia. Lung damage was assessed semi-automatically using Philips Portal v11 COPD software. Rehabilitation measures included therapeutic exercises and chest hardware massage in electrostatic field. therapeutic exercises. RESULTS: Therapeutic exercises significantly reduced severity of lung damage in patients with viral pneumonia CT-2 and no oxygen support (from 28.05% [28; 29.5] at admission to 15.3% [14.2; 19.3] at discharge). It was not observed in patients without rehabilitation treatment and in patients undergoing therapeutic exercises and massage in electrostatic field. CONCLUSION: Therapeutic exercises in patients with COVID-19 and baseline lung damage > 25% and < 50% (CT-2 stage) significantly reduce severity of lung damage at discharge compared to the control group.
Subject(s)
COVID-19 , Pneumonia, Viral , Humans , Lung , Massage , Pneumonia, Viral/complications , Pneumonia, Viral/therapy , Retrospective Studies , SARS-CoV-2 , Static ElectricityABSTRACT
A simple and efficient synthesis of enantiomerically pure (1S,2S)-1,2-di(pyridin-2-yl)- and (1R,2R)-1,2-di(pyridin-4-yl)-ethane-1,2-diamines from commercial picolinaldehyde or isonicotinaldehyde and 2,2'-((1S,2S)-1,2-diaminoethane-1,2-diyl)diphenol (HPEN) via a stereospecific diaza-Cope rearrangement has been developed. Diamine (R,R)-2b was readily converted to novel diastereomeric ionic group-supported bis-prolinamides (R,S)-1b and (R,R)-1b which appeared to be efficient organocatalysts of asymmetric cross-aldol reactions in aldehyde/aldehyde, ketone/ketone or ketone/aldehyde reagent systems under neat conditions to afford corresponding aldols in high yields and with up to 99% ee. Furthermore, catalyst (R,S)-1b could be reused over 15 times in the same or similar catalytic reactions exhibiting close to original activity and a high level of stereoinduction.
ABSTRACT
OBJECTIVES: The recent success of individualized lung cancer therapy has triggered fundamental changes in clinical research strategies. To date there is a strong focus on early proof of concept trials in genetically preselected small patient subgroups. This analysis focuses on the economic burden caused by such trials for advanced lung cancer patients in a German Comprehensive Cancer Center (CCC). METHODS: The profit margins between recruiting groups with ≤3 and >3 patients were compared. Clinical and economic data from clinical trials for advanced lung cancer (LC), pharma-sponsored trials (PhST) as well as investigator initiated trials (IIT), conducted between 2011 and 2015 at the Center for Integrated Oncology (CIO) Cologne, were analyzed using a profit-center calculation model. RESULTS: 161 patients were enrolled in 27 clinical trials. The key economic parameter determining costs and payments was the 'trial visits'. In comparison of the two groups (A≤3; B>3 patients enrolled) we found negative profit margins for the low recruiting group ( -1444). Concerning the number of visits significant differences were found between PhST and IIT (p=0.009). Additionally, sub-analysis show structural differences in cost composition by conducting PhST and IIT. CONCLUSION: Trials with low patient numbers and IIT, do not cover the cost. To ensure adequate, cost-covering compensation by pharmaceutical companies CCCs have to thoroughly calculate the cost of early proof of concept trials. The findings of this study also underline the need for novel structures in public funding for investigator-initiated clinical trials in precision medicine.
Subject(s)
Costs and Cost Analysis , Lung Neoplasms/epidemiology , Aged , Cancer Care Facilities , Clinical Trials as Topic , Female , Germany/epidemiology , Humans , Lung Neoplasms/diagnosis , Male , Middle Aged , Neoplasm Staging , Retrospective Studies , Standard of CareABSTRACT
Novel C2-symmetric N,N'-bis-[(pyrrolidin-2-yl)methyl-squaramide] TFA salts bearing (R,R)- or (S,S)-1,2-di(pyridin-2-yl)ethane spacer groups were synthesized and applied, in combination with TEA, as efficient organocatalysts for asymmetric reactions between cyclohexanone derivatives and ß-nitrostyrenes to afford the corresponding Michael adducts in high yields with good to excellent enantioselectivity. The catalytic procedure is readily scalable and recyclable over four times without a negative impact on the selectivity of the reaction. Some of the prepared compounds are valuable precursors to useful bioactive molecules.
Subject(s)
Pyrrolidines/chemistry , Quinine/analogs & derivatives , Catalysis , Cyclohexanones/chemistry , Quinine/chemistry , Styrene/chemistryABSTRACT
Comprehensive molecular genotyping of lung cancers has become a key requirement for guiding therapeutic decisions. As a paradigm model of implementing next-generation comprehensive diagnostics, Network Genomic Medicine (NGM) has established central diagnostic and clinical trial platforms for centralised testing and decentralised personalised treatment in clinical practice. Here, we describe the structures of the NGM network and give a summary of technologies to identify patients with anaplastic lymphoma kinase (ALK) fusion-positive lung adenocarcinomas. As unifying test platforms will become increasingly important for delivering reliable, quick and affordable tests, the NGM diagnostic platform is currently implementing a comprehensive hybrid capture-based parallel sequencing pan-cancer assay.
Subject(s)
Adenocarcinoma/enzymology , Lung Neoplasms/enzymology , Precision Medicine , Receptor Protein-Tyrosine Kinases/genetics , Adenocarcinoma/diagnosis , Adenocarcinoma/genetics , Adenocarcinoma of Lung , Anaplastic Lymphoma Kinase , Animals , Gene Rearrangement , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Lung Neoplasms/diagnosis , Lung Neoplasms/genetics , Oncogene Proteins, Fusion/geneticsABSTRACT
International demands exist for the patients safety guaranteeng, in the live donors of organs peculiarly, who consciously agreed to be operated on with the organ excision for the patient sake. That's why it is important to elaborate optimal measures of safety for such operative intervention, including anesthesiological support for donors of kidney. Advantages of modern anesthetics application, ingalation introduction of sevofluran and intravenous injection of propophol over standard analgesia were discussed. The investigation was conducted in 53 live familial donors of kidney. Application of the recommended tactics have permitted to reduce the risk of occurrence and rate of complications in these patients.
Subject(s)
Anesthesia, Inhalation/methods , Anesthesia, Intravenous/methods , Living Donors , Methyl Ethers , Nephrectomy/methods , Propofol , Adult , Aged , Heart Function Tests , Humans , Kidney Function Tests , Middle Aged , Monitoring, Intraoperative , Sevoflurane , Treatment OutcomeABSTRACT
Prelamination, permitting improvement not esthetics only, but also function of the damaged region, to create necessary, by contents and configuration, vascularized complexes of tissues, is applied for effective treatment of complex defects in tissues. In experiment on laboratory animals for prelamination of vascularized flaps were applied separate and combined placement of mucosal and cartilaginous autotransplants. Results of the investigation witness possibility and efficacy of creation of content--component complexes of tissues, owing necessary characteristics for their further application in the clinic during performance of reconstructive interventions.
Subject(s)
Abdominal Wall/surgery , Cartilage/transplantation , Gastric Mucosa/transplantation , Myocutaneous Flap/transplantation , Skin Transplantation , Abdominal Wall/blood supply , Abdominal Wall/innervation , Animals , Cartilage/blood supply , Cartilage/innervation , Gastric Mucosa/blood supply , Gastric Mucosa/innervation , Myocutaneous Flap/blood supply , Myocutaneous Flap/innervation , Rats , Skin/blood supply , Skin/innervation , Tissue EngineeringABSTRACT
Diabetes mellitus accompanying tuberculosis complicates the course of the latter and remains a topical problem of phthisiology. Surgical treatment of patient with both conditions presents a high risk and is employed unreasonably rarely. The results of medical (173 patients) and surgical (107 patients) treatments were studied in patients with destructive tuberculosis concurrent with diabetes mellitus. Analyzing early and late results of treatment showed a more steady-state effect in the group of surgically treated patients. Thus, surgery should be desirably used in all patients with preserving destructive changes after the basic course of antibacterial therapy.
Subject(s)
Diabetes Complications , Tuberculosis, Pulmonary/complications , Tuberculosis, Pulmonary/therapy , Adult , Antibiotics, Antitubercular/therapeutic use , Antitubercular Agents/therapeutic use , Diabetes Mellitus, Type 1/complications , Female , Follow-Up Studies , Humans , Isoniazid/therapeutic use , Male , Middle Aged , Pneumonectomy , Rifampin/therapeutic use , Risk Factors , Thoracoplasty , Time Factors , Tuberculoma/complications , Tuberculoma/surgery , Tuberculoma/therapy , Tuberculosis, Pulmonary/drug therapy , Tuberculosis, Pulmonary/mortality , Tuberculosis, Pulmonary/surgeryABSTRACT
New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye 1 was covalently linked to 5'-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye 1 into fluorescent pyridinium cyanine structure 2.
Subject(s)
Fluorescent Dyes/chemistry , Oligonucleotides/chemistry , Staining and Labeling/methods , Thiazoles/chemistry , Amines/chemistry , Chromatography, High Pressure Liquid , Fluorescent Dyes/metabolism , Molecular Structure , Oligonucleotides/metabolism , Thiazoles/metabolismABSTRACT
Commonly seen in liquidators of the effects of the Chernobyl accident are affections of the liver (chronic hepatitis) and bile tract (chronic calculous cholecystitis) accompanied by IHD. The above patients demonstrate enhancement of the antioxidant defence in the blood with the values for free-radical peroxidation remaining unchanged. Absolute values for blood content of LTV4 in chronic active hepatitis were found out to be low, which fact suggests that such patients need to be kept under periodic oncological surveillance.
Subject(s)
Antioxidants/radiation effects , Hepatitis, Chronic/blood , Radiation Injuries/blood , Adult , Antioxidants/analysis , Cholecystitis/blood , Cholecystitis/etiology , Hepatitis, Chronic/etiology , Humans , Leukotriene B4/blood , Leukotriene B4/radiation effects , Lipid Peroxidation/radiation effects , Male , Middle Aged , Power Plants , Radiation Injuries/complications , Radioactive Hazard Release , Time Factors , UkraineABSTRACT
There were 96 patients with various nonpurulent pancreatic gland diseases examined. Postoperative disorders of function of myocardium, liver, kidneys, hormonal-metabolic state, inflammatory and reactive processes in blood, mostly pronounced after partial pancreatic resection conduction, were observed in 49 patients with organic hyperinsulinism having a background of initial changes in homeostasis.
Subject(s)
Insulinoma/surgery , Multiple Organ Failure/etiology , Pancreatic Neoplasms/surgery , Postoperative Complications , Humans , Multiple Organ Failure/diagnosis , Time FactorsABSTRACT
Presence of endocrine disorders, malfunction of liver and kidneys requests special approach application concerning general anaesthetic use in patients with the hormone producing insulinomas. There were 44 patients examined, in whom tumor was removed. Sodium hydroxybutyrate application while introducing anaesthesia promoted the haemodynamics stabilization. Arduan dosage ought to be reduced because of it possible cumulation in blood due to hepatorenal disorders existing in this patients. The dominance of the blood glucagon at the end of operation and the glucagon-insulin coefficient shift causes the tachycardia and the shock index increase. The proposed direct binasal stimulation of respiration after the anaesthesia conduction promoted rapid muscle tone and respiratory movements restoration in patients.
Subject(s)
Anesthesia, General , Insulinoma/surgery , Neuroleptanalgesia , Pancreatic Neoplasms/surgery , Glucagon/blood , Hemodynamics , Humans , Insulin/blood , Neuromuscular Nondepolarizing Agents/administration & dosage , Pipecuronium/administration & dosageSubject(s)
Anesthesia/methods , Insulinoma/blood , Pancreatic Neoplasms/blood , Preoperative Care , Biomarkers/blood , Female , Humans , Insulinoma/physiopathology , Insulinoma/surgery , Kidney/physiopathology , Liver/physiopathology , Male , Pancreatic Neoplasms/physiopathology , Pancreatic Neoplasms/surgery , Risk FactorsSubject(s)
Lung Diseases , Mediastinal Diseases , Sarcoidosis , Adrenal Cortex Hormones/therapeutic use , Adult , Anabolic Agents/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Diagnosis, Differential , Diet , Female , Follow-Up Studies , Hospitals, Special , Humans , Hydrocortisone/administration & dosage , Lung Diseases/diagnosis , Lung Diseases/therapy , Male , Mediastinal Diseases/diagnosis , Mediastinal Diseases/therapy , Middle Aged , Nandrolone/analogs & derivatives , Nandrolone/therapeutic use , Nandrolone Decanoate , Respiratory Therapy , Sarcoidosis/diagnosis , Sarcoidosis/therapy , Time FactorsABSTRACT
The influence of bull fathers' pedigree values and the influence of the type of the selection have been studied from the viewpoint of heritability of the selective traits in "father-son-grandfather" generations. The trend of positive influence of inbreeding on bulls' pedigree values has been studied from the viewpoint of milk yield in their daughters.