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Stem Cell Res
; 57: 102586, 2021 Oct 26.
Article
in English
| MEDLINE
| ID: mdl-34736039
ABSTRACT
In the canonical WNT signaling pathway, active WNT signaling results in the nuclear translocation of ß-catenin where it regulates target gene expression. As a tool to understand these ß-catenin DNA interactions, we used a CRISPR/Cas9 based approach to engineer a human embryonic stem cell line (hESC) harboring a 3X FLAG sequence fused to the C-terminus of ß-catenin. Engineered cells displayed a characteristic hESC morphology, expressed pluripotency-associated markers, retained tri-lineage differentiation potential, and had a normal euploid karyotype. This cell line represents a valuable tool to dissect the transcriptional mechanisms by which WNT signalling regulates pluripotent cell fate.