ABSTRACT
Synaptogyrins are conserved components of the exocytic apparatus and function as regulators of Ca(2+)-dependent exocytosis. The synaptogyrin family comprises three isoforms: two neuronal (synaptogyrin-1 and -3) and one ubiquitous (synaptogyrin-2) form. Although the expression patterns of the exocytic proteins synaptotagmin-1, SNAP-25, synaptobrevin-2 and synaptophysin have been elucidated in taste buds, the function and expression pattern of synaptogyrin-1 in rat gustatory tissues have not been determined. Therefore, we examined the expression patterns of synaptogyrin-1 and several cell-specific markers of type II and III cells in rat gustatory tissues. Reverse transcription/polymerase chain reaction assays and immunoblot analysis revealed the expression of synaptogyrin-1 mRNA and its protein in circumvallate papillae. In fungiform, foliate and circumvallate papillae, the antibody against synaptogyrin-1 immunolabeled a subset of taste bud cells and intra- and subgemmal nerve processes. Double-labeling experiments revealed the expression of synaptogyrin-1 in most taste cells immunoreactive for aromatic L-amino acid decarboxylase and the neural cell adhesion molecule. A subset of synaptogyrin-1-immunoreactive taste cells also expressed phospholipase Cß2, gustducin, or sweet taste receptor (T1R2). In addition, most synaptogyrin-1-immunoreactive taste cells expressed synaptobrevin-2. These results suggest that synaptogyrin-1 plays a regulatory role in transmission at the synapses of type III cells and is involved in exocytic function with synaptobrevin-2 in a subset of type II cells in rat taste buds.
Subject(s)
Gene Expression Regulation/physiology , Receptors, G-Protein-Coupled/biosynthesis , Synaptogyrins/biosynthesis , Taste Buds/metabolism , Animals , Exocytosis/physiology , Male , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Signal Transduction/physiology , Taste Buds/cytology , Vesicle-Associated Membrane Protein 2/biosynthesisABSTRACT
Properties of the Olami-Feder-Christensen (OFC) model of earthquakes are studied by numerical simulations. The previous study indicated that the model exhibited "asperity"-like phenomena, i.e., the same region ruptures many times near periodically [T. Kotani, Phys. Rev. E 77, 010102(R) (2008)]. Such periodic or characteristic features apparently coexist with power-law-like critical features, e.g., the Gutenberg-Richter law observed in the size distribution. In order to clarify the origin and the nature of the asperity-like phenomena, we investigate here the properties of the OFC model with emphasis on its stress distribution. It is found that the asperity formation is accompanied by self-organization of the highly concentrated stress state. Such stress organization naturally provides the mechanism underlying our observation that a series of asperity events repeat with a common epicenter site and with a common period solely determined by the transmission parameter of the model. Asperity events tend to cluster both in time and in space.
Subject(s)
Algorithms , Earthquakes , Models, Statistical , Oscillometry/methods , Computer SimulationABSTRACT
Characteristic versus critical features of earthquakes are studied on the basis of the Olami-Feder-Christensen model. It is found that the local recurrence-time distribution exhibits a sharp delta -function-like peak corresponding to rhythmic recurrence of events with a fixed "period" uniquely determined by the transmission parameter of the model, together with a power-law-like tail corresponding to scale-free recurrence of events. The model exhibits phenomena closely resembling the asperity known in seismology.
