ABSTRACT
Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.
Subject(s)
Cell Differentiation , Mucous Membrane/cytology , Muscle Development , Pulmonary Alveoli/cytology , Adipogenesis , Adult , Cell Shape , Chondrogenesis , Female , Gingiva/cytology , Humans , Karyotyping , Male , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Mesoderm/cytology , Middle Aged , Myocytes, Smooth Muscle/cytology , Myofibroblasts/cytology , Myofibroblasts/metabolism , Osteogenesis , Pulmonary Alveoli/metabolismABSTRACT
Cervical artery dissection (CAD) is one of the most frequent causes (14.5%) of stroke in young adults. Cases with involvement of more than two arteries are rare. Arnold et al described 11 cases (1.5%) with triple CAD of a reported 740 patients and just a single (0.1%) quadruple case in the same population. Simultaneous dissection of the four principal vessels is extremely rare. According to Papagiannaki et al, the incidence of simultaneous three or four CADs is 1-3/million in the general population. To the best of our knowledge, there are only three published cases of spontaneous quadruple CAD.
Subject(s)
Carotid Artery Thrombosis/diagnostic imaging , Carotid Artery, Internal, Dissection/diagnostic imaging , Carotid Artery, Internal/diagnostic imaging , Thrombophilia/diagnosis , Vertebral Artery Dissection/diagnostic imaging , Adult , Angiography, Digital Subtraction , Antithrombin III/therapeutic use , Antithrombins/therapeutic use , Carotid Artery Thrombosis/etiology , Carotid Artery Thrombosis/surgery , Carotid Artery, Internal/surgery , Carotid Artery, Internal, Dissection/etiology , Carotid Artery, Internal, Dissection/surgery , Cerebral Angiography , Female , Humans , Stents , Thrombophilia/complications , Thrombophilia/drug therapy , Vertebral Artery Dissection/etiology , Vertebral Artery Dissection/surgeryABSTRACT
A comparative investigation of the induction of double-strand DNA breaks (DSBs) in the Chinese hamster V79 cells by γ-radiation at dose rates of 1, 10 and 400 mGy/min (doses ranged from 0.36 to 4.32 Gy) was performed. The acute radiation exposure at a dose rate of 400 mGy/min resulted in the linear dose-dependent increase of the γ-H2AX foci formation. The dose-response curve for the acute exposure was well described by a linear function y = 1.22 + 19.7x, where "y" is an average number of γ-H2AX foci per a cell and "x" is the absorbed dose (Gy). The dose rate reduction down to 10 mGy/min lead to a decreased number of γ-H2AX foci, as well as to a change of the dose-response relationship. Thus, the foci number up to 1.44 Gy increased and reached the "plateau" area between 1.44 and 4.32 Gy. There was only a slight increase of the γ-H2AX foci number (up to 7) in cells after the protracted exposure (up to 72 h) to ionizing radiation at a dose rate of 1 mGy/min. Similar effects of the varying dose rates were obtained when DNA damage was assessed using the comet assay. In general, our results show that the reduction of the radiation dose rate resulted in a significant decrease of DSBs per cell per an absorbed dose.
