Evidence of coinfection with distinct strains of Burkholderia multivorans in a cystic fibrosis patient.

We report a Cystic Fibrosis patient with chronic Burkholderia multivorans infection involving persistency of one strain and temporary, consecutive coinfection with two different strains. Comparison of the colony morphology with the genotype revealed no correlation. These data are important for interpretation of clinical outcome and transmission studies in CF patients.

[Light and scanning electron microscopic studies of structure of dentin interglobular spaces (author's transl)]. / Licht-und rasterelektronenmikroskopische Untersuchungen zur Struktur der Interglobulararäume.

The author, after giving a literature survey, reports the results of scanning electron microscope examinations of interglobular spaces. The interglobular spaces are mineral-free regions within the dentin. They contain an organic matrix with a smaller amount of ground substance and a smaller number of collagenic fibrils than are contained in the matrix of mineralized dentin. Obviously, hollow spaces observed during microscopic examination are artifacts produced by shrinkage of the matrix despite good fixation. It has not so far been possible to obtain any indications of liquid-filled spaces.