1.
Asian Pac J Allergy Immunol
; 18(1): 9-14, 2000 Mar.
Article
in English
| MEDLINE
| ID: mdl-12546052
ABSTRACT
Allergenic components of cowpea vegetable green seeds (Vigna sinensis) were isolated based on solubility, isoelectric precipitation and molecular mass. The allergenicity of the cowpea fractions was monitored by enzyme-linked immunosorbent assay (ELISA) and skin-prick test. The allergenic albumin fraction was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-specific immunoblotting. The 41 and 55 kDa protein components were found to be major allergens and the allergenicity was resistant to heat and proteolytic enzyme digestion. This study confirms the presence of potent allergens in cowpea seeds.
Subject(s)
Allergens/isolation & purification , Fabaceae/immunology , Food Hypersensitivity/immunology , Adolescent , Adult , Allergens/chemistry , Child , Chromatography, Gel , Enzyme-Linked Immunosorbent Assay , Fabaceae/chemistry , Humans , Immunoblotting , Immunoglobulin E/blood , Middle Aged , Molecular Weight , Plant Proteins, Dietary/chemistry , Plant Proteins, Dietary/immunology , Plant Proteins, Dietary/isolation & purification , Seeds/chemistry , Seeds/immunology
2.
Chest
; 67(2): 217-8, 1975 Feb.
Article
in English
| MEDLINE
| ID: mdl-1116398
Subject(s)
Lung Abscess/diagnostic imaging , Pneumonia, Staphylococcal/diagnostic imaging , Adult , Diagnosis, Differential , Gentamicins/therapeutic use , Humans , Lung Abscess/drug therapy , Lung Abscess/etiology , Male , Microbial Sensitivity Tests , Pneumonia, Staphylococcal/drug therapy , Radiography , Sepsis/etiology , Streptococcal Infections/diagnostic imaging , Streptomycin/therapeutic use
3.
Indian J Chest Dis
; 16(4): 411-4, 1974 Oct.
Article
in English
| MEDLINE
| ID: mdl-4469269
4.
Indian J Chest Dis
; 16(3): 182-7, 1974 Jul.
Article
in English
| MEDLINE
| ID: mdl-4423506
Subject(s)
Liver/physiopathology , Respiratory Tract Diseases/physiopathology , Adolescent , Adult , Aged , Child , Chronic Disease , Clinical Enzyme Tests , Female , Humans , Liver Diseases/diagnosis , Liver Function Tests , Lung Neoplasms/physiopathology , Male , Middle Aged , Respiratory Hypersensitivity/physiopathology
5.
Indian J Chest Dis
; 16(3): 171-81, 1974 Jul.
Article
in English
| MEDLINE
| ID: mdl-4423511
6.
Indian J Chest Dis
; 15(3): 231-5, 1973 Jul.
Article
in English
| MEDLINE
| ID: mdl-4753817
