ABSTRACT
The cloning and sequencing of Saccharomyces cerevisiae ADE12 gene encoding the structure of adenylsuccinate synthetase (ASS) are reported for the first time. Comparative analysis of all known ASS sequences was carried out. Regulation of ADE12 gene expression by exogenic adenine was carried out. The properties of ASS which was isolated and purified from overproducing yeast strain were examined.
Subject(s)
Adenylosuccinate Synthase/genetics , Saccharomyces cerevisiae/genetics , Adenylosuccinate Synthase/isolation & purification , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA Probes , DNA, Recombinant , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Fungal , Molecular Sequence Data , Saccharomyces cerevisiae/enzymology , Sequence Homology, Amino AcidABSTRACT
A series of methods was developed for improving the reliability of chromatographic analyses and to augment their information content and reproducibility. In high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) the possibility of utilizing azeotropic and isobaric solvent systems was substantiated. To control variations of the composition of the eluent systems during storage and analysis in TLC and HPLC, gas chromatography (GC) was found to be applicable. To improve the reliability of GC analyses, a membraneless assembly for introduction of samples, which allows false peaks in a chromatogram to be removed, is recommended. In order to augment the reliability and information content of chromatographic analyses of several bioactive compounds, complex chromatographic methods based on GC, TLC and HPLC were found to be desirable.
