Removal of contaminants of emerging concern by Wolffia arrhiza and Lemna minor depending on the process conditions, pollutants concentration, and matrix type.

Research was carried out on the removal of a group of six contaminants of emerging concern: bisphenol A, N,N-diethyl-m-toluamide, diethylstilbestrol, triclosan, estrone and estradiol from the water matrix during contact with small floating macrophytes Wolffia arrhiza and Lemna minor. The optimal conditions for the process, such as pH, light exposure per day, and plant mass, were determined using the design of experiments chemometric approach based on central composite design. Experiments conducted under the designated optimal conditions showed that after 7 days, the removal efficiency equals 88-98% in the case of W. arrhiza and 87-97% in the case of L. minor, while after 14 days of the experiment, these values are 93-99.6% and 89-98%, respectively. The primary mechanism responsible for removing CECs is the plant uptake, with the mean uptake rate constant equal to 0.299 day-1 and 0.277 day-1 for W. arrhiza and L. minor, respectively. Experiments conducted using municipal wastewater as a sample matrix showed that the treatment efficiency remains high (the average values 84% and 75%; in the case of raw wastewater, 93% and 89%, and in the case of treated wastewater, for W. arrhiza and L. minor, respectively). Landfill leachate significantly reduces plants' ability to remove pollutants (the average removal efficiency equals 59% and 56%, for W. arrhiza and L. minor, respectively).

Design and Evaluation of Low-Cost Vibration-Based Machine Monitoring System for Hay Rotary Tedder.

Vibration monitoring provides a good-quality source of information about the health condition of machines, and it is often based on the use of accelerometers. This article focuses on the use of accelerometer sensors in fabricating a low-cost system for monitoring vibrations in agricultural machines, such as rotary tedders. The aim of the study is to provide useful data on equipment health for improving the durability of such machinery. The electronic prototype, based on the low-cost AVR microcontroller ATmega128 with 10-bit ADC performing a 12-bit measurement, is able to acquire data from an accelerometer weighing up to 10 g. Three sensors were exposed to low accelerations with the use of an exciter, and their static characteristics were presented. Standard experimental tests were used to evaluate the constructed machine monitoring system. The self-contained prototype system was calibrated in a laboratory test rig, and sinusoidal and multisinusoidal excitations were used. Measurements in time and frequency domains were carried out. The amplitude characteristic of the preformed system differed by no more than 15% within a frequency range of 10 Hz-10 kHz, compared to the AVM4000 commercial product. Finally, the system was experimentally tested to measure acceleration at three characteristic points in a rotational tedder, i.e., the solid grease gearbox, the drive shaft bearing and the main frame. The RMS amplitude values of the shaft vibrations on the bearing in relation to the change in the drive shaft speed of two tedders of the same type were evaluated and compared. Additionally, the parameters of kurtosis and crest factor were compared to ascertain the bearing condition.

Emerging therapies in pancreas cancer.

Pancreas cancer has a grave prognosis and treatment options remain limited despite advancement in anti-cancer chemotherapeutics. This review provides an overview of the emerging therapies for pancreas cancer, focusing on novel signal transduction inhibitors (insulin-like growth factor receptor, hedgehog/Smo, PI3k/Akt/mTOR) and cytotoxics (nab-paclitaxel) that are currently in clinical development. Despite the impact molecularly targeted agents have on other tumor types, their application without cytotoxics in pancreas cancer remains limited. In addition, recent report of the superiority of an intensive cytotoxic regimen using fluorouracil, irinotecan and oxaliplatin (FOLFIRINOX) over gemcitabine reminded us of the importance of cytotoxics in this disease. As such, the future of pancreas cancer therapy may be combination regimens consisting of cytotoxics and molecularly targeted agents.

Acquirement of rituximab resistance in lymphoma cell lines is associated with both global CD20 gene and protein down-regulation regulated at the pretranscriptional and posttranscriptional levels.

Acquirement of resistance to rituximab has been observed in lymphoma patients. To define mechanisms associated with rituximab resistance, we developed various rituximab-resistant cell lines (RRCL) and studied changes in CD20 expression/structure, lipid raft domain (LRD) reorganization, calcium mobilization, antibody-dependent cellular cytotoxicity, and complement-mediated cytotoxicity (CMC) between parental and RRCL. Significant changes in surface CD20 antigen expression were shown in RRCL. Decreased calcium mobilization and redistribution of CD20 into LRD were found in RRCL. Western blotting identified a unique 35 kDa protein band in RRCL, which was not seen in parental cells and was secondary to an increase in surface and cytoplasmic expression of IgM light chains. CD20 gene expression was decreased in RRCL. In vitro exposure to PS341 increased CD20 expression in RRCL and minimally improved the sensitivity to rituximab-associated CMC. Our data strongly suggest that the acquisition of rituximab resistance is associated with global gene and protein down-regulation of the CD20 antigen affecting LRD organization and downstream signaling. CD20 expression seems to be regulated at the pretranscriptional and posttranscriptional levels. Proteasome inhibition partially reversed rituximab resistance, suggesting the existence of additional mediators of rituximab resistance. Future research is geared to identify drugs and/or biological agents that are effective against RRCL.