ABSTRACT
The isolates of microorganisms from the rhizosphere of spring barley plants and soil at the use of analytical selection method was isolated. Its isolates on the ability of "nitrogen-fixing activity" was tested. It was shown that isolates of microorganisms had different of the colonies formed and cultural growth on the Eshbi's selective medium as well as the ability to fixing of molecular nitrogen. The different levels of intensity and dynamics of isolates nitrogenase activity in vitro were identified. New isolates of the soil microorganisms complement of the gene pool diazotrophic bacteria. Its isolates are perspectivity for the study as the basis or components of the bacterial fertilizers for the crops.
Subject(s)
Bacteria/isolation & purification , Bacterial Proteins/metabolism , Hordeum/microbiology , Nitrogenase/metabolism , Soil Microbiology , Bacteria/enzymology , Bacteria/genetics , Bacterial Proteins/genetics , Crops, Agricultural/growth & development , Crops, Agricultural/microbiology , Fertilizers/supply & distribution , Gene Expression , Gene Pool , Hordeum/growth & development , Microbial Consortia/genetics , Nitrogen/metabolism , Nitrogen Fixation/physiology , Nitrogenase/genetics , Rhizosphere , Symbiosis/physiologyABSTRACT
Seed inoculation with bacterial consortium was found to increase legume yield, providing a higher growth than the standard nitrogen treatment methods. Alfalfa plants were inoculated by mono- and binary compositions of nitrogen-fixing microorganisms. Their physiological and biochemical properties were estimated. Inoculation by microbial consortium of Sinorhizobium meliloti T17 together with a new cyanobacterial isolate Nostoc PTV was more efficient than the single-rhizobium strain inoculation. This treatment provides an intensification of the processes of biological nitrogen fixation by rhizobia bacteria in the root nodules and an intensification of plant photosynthesis. Inoculation by bacterial consortium stimulates growth of plant mass and rhizogenesis and leads to increased productivity of alfalfa and to improving the amino acid composition of plant leaves. The full nucleotide sequence of the rRNA gene cluster and partial sequence of the dinitrogenase reductase (nifH) gene of Nostoc PTV were deposited to GenBank (JQ259185.1, JQ259186.1). Comparison of these gene sequences of Nostoc PTV with all sequences present at the GenBank shows that this cyanobacterial strain does not have 100% identity with any organisms investigated previously. Phylogenetic analysis showed that this cyanobacterium clustered with high credibility values with Nostoc muscorum.
Subject(s)
Cyanobacteria/physiology , Medicago sativa/physiology , Nitrogen Fixation/physiology , Root Nodules, Plant/microbiology , Symbiosis , Agriculture , Cyanobacteria/metabolism , Medicago sativa/metabolism , Molecular Sequence Data , Nitrogen/metabolism , Phylogeny , Rhizobium/metabolism , Rhizobium/physiology , Root Nodules, Plant/physiology , Sinorhizobium meliloti/metabolism , Sinorhizobium meliloti/physiologyABSTRACT
The present review contains results of proteomic researches of legume-rhizobium symbiosis. The technical difficulties associated with the methods of obtaining protein extracts from symbiotic structures and ways of overcoming them were discussed. The changes of protein synthesis under formation and functioning of symbiotic structures were shown. Special attention has been given to the importance of proteomic studies of plant-microbe structures in the formation of adaptation strategies under adverse environmental conditions. The technical and conceptual perspectives of legume-rhizobium symbiosis proteomics were shown.
