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J Sci Food Agric ; 94(7): 1349-58, 2014 May.
Article in English | MEDLINE | ID: mdl-24114525

ABSTRACT

BACKGROUND: Heat pretreatment is considered the first step in grain milling. This study therefore evaluated microwave and micronization heat treatments in improving the dehulling characteristics, phenolic composition and antioxidant and α-amylase activities of bean cultivars from three market classes. RESULTS: Heat treatments improved dehulling characteristics (hull yield, rate coefficient and reduced abrasive hardness index) depending on bean cultivar, whereas treatment effects increased with dehulling time. Micronization increased minor phenolic components (tartaric esters, flavonols and anthocyanins) of all beans but had variable effects on total phenolic content depending on market class. Microwave treatment increased α-amylase inhibitor concentration, activity and potency, which were strongly correlated (r² = 0.71, P < 0.0001) with the flavonol content of beans. Heat treatment had variable effects on the phenolic composition of bean hulls obtained by abrasive dehulling without significantly altering the antioxidant activity of black and pinto bean hulls. Principal component analysis on 22 constituents analyzed in this study demonstrated the differences in dehulling characteristics and phenolic components of beans and hulls as major factors in segregating the beneficial heat treatment effects. CONCLUSION: Heat treatment may be useful in developing novel dietary fibers from beans with variable composition and bioactivity with a considerable range of applications as functional food ingredients.


Subject(s)
Food Handling , Food, Preserved/analysis , Functional Food/analysis , Industrial Waste/analysis , Phaseolus/chemistry , Plant Epidermis/chemistry , Seeds/chemistry , Alberta , Antioxidants/analysis , Antioxidants/economics , Antioxidants/radiation effects , Food, Fortified/analysis , Food, Fortified/economics , Food, Preserved/radiation effects , Food-Processing Industry/economics , Functional Food/radiation effects , Hot Temperature , Industrial Waste/economics , Infrared Rays , Mechanical Phenomena , Microwaves , Phaseolus/growth & development , Phaseolus/metabolism , Phaseolus/radiation effects , Phenols/analysis , Phenols/economics , Phenols/radiation effects , Pigmentation/radiation effects , Plant Epidermis/growth & development , Plant Epidermis/metabolism , Plant Epidermis/radiation effects , Plant Lectins/metabolism , Plant Lectins/radiation effects , Plant Proteins/metabolism , Plant Proteins/radiation effects , Saskatchewan , Seeds/growth & development , Seeds/metabolism , Seeds/radiation effects , Washington , alpha-Amylases/antagonists & inhibitors , alpha-Amylases/metabolism , alpha-Amylases/radiation effects
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