ABSTRACT
OBJECTIVES: A multicentric randomized, 3-year prospective study was conducted to determine for how long Biodentine, a new biocompatible dentine substitute, can remain as a posterior restoration. MATERIALS AND METHODS: First, Biodentine was compared to the composite Z100®, to evaluate whether and for how long it could be used as a posterior restoration according to selected United States Public Health Service (USPHS)' criteria (mean ± SD). Second, when abrasion occurred, Biodentine was evaluated as a dentine substitute combined with Z100®. RESULTS: A total of 397 cases were included. This interim analysis was conducted on 212 cases that were seen for the 1-year recall. On the day of restoration placement, both materials obtained good scores for material handling, anatomic form (0.12 ± 0.33), marginal adaptation (0.01 ± 0.10) and interproximal contact (0.11 ± 0.39). During the follow-up, both materials scored well in surface roughness (≤1) without secondary decay and post-operative pain. Biodentine kept acceptable surface properties regarding anatomic form score (≤1), marginal adaptation score (≤2) and interproximal contact score (≤1) for up to 6 months after placement. Resistance to marginal discoloration was superior with Biodentine compared to Z100®. When Biodentine was retained as a dentine substitute after pulp vitality control, it was covered systematically with the composite Z100®. This procedure yielded restorations that were clinically sound and symptom free. CONCLUSIONS: Biodentine is able to restore posterior teeth for up to 6 months. When subsequently covered with Z100®, it is a convenient, efficient and well tolerated dentine substitute. CLINICAL RELEVANCE: Biodentine as a dentine substitute can be used under a composite for posterior restorations.
Subject(s)
Biocompatible Materials/standards , Calcium Compounds/standards , Dental Materials/standards , Dental Restoration, Permanent/standards , Dentin/anatomy & histology , Silicates/standards , Adult , Biocompatible Materials/chemistry , Bite Force , Calcium Compounds/chemistry , Color , Composite Resins/chemistry , Dental Caries/prevention & control , Dental Cavity Preparation/classification , Dental Marginal Adaptation , Dental Materials/chemistry , Dental Restoration Failure , Dental Restoration Repair , Dental Restoration Wear , Dental Restoration, Permanent/classification , Dentin Sensitivity/prevention & control , Follow-Up Studies , Humans , Prospective Studies , Pulp Capping and Pulpectomy Agents/chemistry , Silicates/chemistry , Silicon Dioxide/chemistry , Surface Properties , Zirconium/chemistryABSTRACT
OBJECTIVES: To evaluate the cytotoxicity of Shellac F, a new fluoride varnish, and its effects on human dentin hydraulic conductance. METHODS: Shellac F was compared to another fluoride varnish (Duraphat) and a fluoride containing desensitizing agent (Isodan). The cytotoxicity test was performed on human gingival fibroblasts and through dentin slice on human pulp fibroblasts. The hydraulic conductance (Lp) was recorded by fluid filtration with a Flodec device under a constant pressure (15 cm H2O). The treated surface of the dentin disks and their sections were also investigated with SEM. RESULTS: The cytotoxicity test on gingival fibroblasts revealed that Duraphat was the least cytotoxic material, followed by Shellac F then Isodan. With dentin slice interposition, a lower level of cytotoxicity was obtained. All of them showed a lower cytotoxicity decreasing on further dilutions (p<0.001). The measurement of hydraulic conductance showed that all materials resulted in a significant decrease in dentin permeability after 24h comprising between 60 and 76%, but there was no statistically significant difference among the materials. This decrease was still over 50% of the initial values after 7 days for all three materials. SEM investigation showed dentin tubules covered with a thick layer of Shellac F or Duraphat whilst no material was observed on dentin surfaces treated with Isodan. SIGNIFICANCE: Shellac F showed an adequate cellular compatibility and a significant effect on human dentin hydraulic conductance. This indicates that the new material is safe and seems to be effective as a potential desensitizing agent.
Subject(s)
Dentin Sensitivity/physiopathology , Fluorides, Topical/pharmacology , Sodium Fluoride/pharmacology , Cells, Cultured , Dental Pulp/drug effects , Dentin/drug effects , Dentin/ultrastructure , Dentin Permeability/drug effects , Drug Evaluation, Preclinical , Fibroblasts/drug effects , Fluorides, Topical/toxicity , Gingiva/drug effects , Humans , Materials Testing , Methacrylates/pharmacology , Methacrylates/toxicity , Microscopy, Electron, Scanning , Nitrates/pharmacology , Nitrates/toxicity , Pressure , Sodium Fluoride/toxicity , Time FactorsABSTRACT
This study was undertaken to understand the biodegradation mechanisms of calcium phosphate (Ca-P) biomaterials with different crystallization. Two types of sintered Ca-P porous ceramic (HA and beta-TCP) and a Ca-P bone cement (CPC) were implanted into cavities drilled in rabbit femoral and tibiae condyles. The results have shown that a material biodegradation was rapid in the beta-TCP and the CPC, but very weak in the HA. This biodegradation presented a decrease of material volume from the periphery to the center as well as a particle formation causing phagocytosis by numerous macrophages and multinucleated giant cells in the CPC. In the beta-TCP, there was a peripheral and central decrease of material volume as well as an absence of particle formation or visible phagocytosis. The process of biodegradation is considered to be directly influenced by the type of material crystallization. The sintered bioceramics processed at a high temperature exhibit good crystallization and are primarily degraded by a process dependent on interstitial liquids. However, the bone cement is formed by physicochemical crystallization and is degraded through a dissolution process associated with a cellular process.
