ABSTRACT
Three hundreds percutaneous renal biopsy cases were analyzed for recorded clinical complications retrospectively. Incidence of fever (> or = 37.5 degrees C), pain, and decrease in hematocrit (> or = 4%) was 21%, 28%, and 13% respectively. The incidence of fever and pain were less in the older patients. Prospectively, 125 cases who underwent ultrasonography following renal biopsies and 62 cases who underwent computerized tomography were analyzed. Bigger hematoma was followed by fever 5-7 days after renal biopsies. Incidence of pain and the decrease in hematocrit were correlated with the size of hematoma. Use of the ultrasonographic localization and a Tru-Cut biopsy needle or a Biopty-Cut needle decreased the incidence of hematoma more than 50ml following renal biopsy (0-5%). Incidence of fever and pain was less by these new procedure. The risk of hematoma increased as the biopsy needle went deeper into medulla.
Subject(s)
Biopsy, Needle/adverse effects , Hematoma/etiology , Kidney Diseases/etiology , Kidney/pathology , Adolescent , Adult , Aged , Biopsy, Needle/methods , Child , Female , Fever/etiology , Hematoma/diagnostic imaging , Humans , Kidney Diseases/diagnostic imaging , Male , Retrospective Studies , Tomography, X-Ray Computed , UltrasonographyABSTRACT
We assessed the clinical usefulness of Biopty biopsy instrument & Biopty biopsy needle in percutaneous renal biopsy (PRB) compared with Tru-cut disposable needle and Vim-Silvermann needle. Sixty cases, each consisting 20 cases, were performed PRB by 3 different needles. There was no significant differences between Biopt y-cut needle and Tru-cut needle in the length of renal biopsy tissue and number of glomeruli obtained. The frequency of clinical complications such as fever, flank pain and decrease in Ht greater than 2% was lower in Biopty needle group after PRB. The frequency of middle and large size of hematoma was also lower in Biopty needle group after PRB. We could also obtain specimen from transplanted kidney without complications except small hematoma. From three results, Biopty biopsy needle is a useful tool in performing PRB.
Subject(s)
Biopsy, Needle/methods , Kidney/pathology , Adolescent , Adult , Aged , Biopsy, Needle/adverse effects , Biopsy, Needle/instrumentation , Child , Evaluation Studies as Topic , Female , Hematoma/etiology , Humans , Kidney Diseases/pathology , Male , Middle AgedSubject(s)
Biopsy/adverse effects , Hematoma/diagnosis , Kidney Diseases/diagnosis , Kidney/pathology , Tomography, X-Ray Computed , Ultrasonography , Adolescent , Adult , Aged , Child , Female , Hematoma/etiology , Humans , Kidney Diseases/etiology , Male , Middle AgedABSTRACT
Protein kinase C was purified from rat brain cytosol by using a high performance liquid chromatography (HPLC), Pharmacia FPLC system. This procedure employed a column chromatography on DE-52, followed by three steps of HPLC procedures with threonine-Sepharose (prepared as described in this report), TSK gel Phenyl-5PW (Toyo Soda), and TSK gel G3000SW (Toyo Soda) columns. Starting from about 30 g of rat brain, approximately 200 micrograms of pure enzyme was obtained. The procedure was very simple and highly reproducible. The enzyme thus obtained was nearly pure by silver staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In the presence of 10% (w/v) glycerol and 0.05% (w/v) Triton X-100, the enzyme could be stored at -80 degrees C for several months.
