ABSTRACT
The unregulated expulsion of untreated or partially treated industrial effluents poses serious threat to the aquatic ecosystem. Therefore, in the present study fish Channa punctata were exposed to untreated and microbially treated equalization tank effluent of textile industry and toxicity studies were carried out for 45 days. The study was planned to analyze the toxicity proffered by textile effluents through haematological, biochemical, histopathological and ultrastructural analysis in blood, liver and gill tissues of fish. While comparing untreated and microbially treated effluent exposed groups haematological parameters were significantly (P ≤ 0.05) less in the untreated effluent exposed group whereas White blood cell count was highly escalated. However, in the microbially treated groups, the alterations were less severe. Increased malondialdehyde content indicating oxidative stress, reduced Catalase (CAT) and Superoxide dismutase (SOD) activity showing a weakened antioxidant defence system and increased glutathione activity was also perceived in untreated effluent exposed groups in comparison to microbially treated groups. Histopathological alterations in gill (telangiectasia, lamellae fusion, breakage, vacuolization and bending of lamellae) and liver (sinusoid dilations, fusion, necrosis and congestion) were more pronounced and severe in the untreated effluent exposed group as compared to microbially treated group. The results observed in histopathology were further reaffirmed by scanning electron microscopy. The study clearly highlights less alterations and deformities in microbially treated effluent groups in comparison to untreated effluent groups. These findings, therefore, necessitate the search for more effective microbial inocula for the better treatment of effluents in order to protect the aquatic life as well as human beings. Highlights: Channa punctata exposed for 15, 30 and 45 days to untreated and microbially treated equalization tank effluent of textile industry.Untreated and microbially treated effluent exposed fish elicited alterations in blood, liver and gill tissuesHaematology, biochemical, histopathology and ultrastructural analysis resulted in massive pathologies in groups subjected to untreated effluent inducing maximum damage after 45 days of exposure.Less pronounced toxicity in fish C. punctata was observed in fish exposed to microbially treated effluent indicating its efficacy in toxicity reduction.
ABSTRACT
The unregulated expulsion of untreated textile water into water bodies is a major hazard to aquatic ecosystems. The present investigation was contrived to estimate the impact of textile dye bath effluent (untreated and microbially treated) on fish Channa punctata. Untreated effluent-exposed fish showed extremely altered behaviour (air gulping, erratic and speedy movements, increased opercular activity) and morphology (deposition of dyes on skin and scales, high pigmentation, mucus exudation). Significantly increased micronuclei (1.61-, 1.28-, 1.38-fold) and aberrant cell frequency (1.37-, 1.45-, 1.28-fold) was observed in untreated group as compared to treated group after 15, 30, and 45 days of exposure. Tail length, % tail intensity, tail moment and olive tail moment were also enhanced in all the exposed tissues. However, maximum damage was noticed in gill tissues showing 1.19-, 1.37-, 1.34- and 1.50-fold increased TL, %TI, TM and OTM in untreated group as compared to treated group after 45 days of exposure. On comparing untreated and treated groups, increased blood parameters and significantly reduced white blood cell count (WBC) were noticed in treated group. Significantly enhanced alterations in biochemical parameters were also analysed in untreated group. Reduced alterations in enzymological levels of fishes exposed to treated effluent indicate lesser toxic nature of the degraded metabolites of dye. Histological analysis in fishes exposed to untreated effluent showed several deformities in liver (necrosis, congestion, fusion of cells and melanomacrophage infiltration) and gill tissues (necrosis, bending of lamellae and severe aneurysm). Scanning electron microscopy (SEM) analysis further reaffirmed the pathologies observed in histological analysis. Fewer structural alterations were noticed in treated effluent fishes. The results concluded that untreated effluent inflicted toxicity potential on morphology as well as physiological defects in fish, and the severity increased with increasing duration of exposure, whereas reduction in toxicity in microbially treated groups can be analysed for aquacultural purposes owing to their lesser toxic nature.
Subject(s)
Water Pollutants, Chemical , Animals , Water Pollutants, Chemical/analysis , Textile Industry , Ecosystem , Fishes/metabolism , Necrosis , DNA Damage , Fresh Water , Water/metabolismABSTRACT
The imprudent use of insecticides causes the development of resistance in insect pest populations, contamination of the environment, biological imbalance and human intoxication. The use of microbial pathogens combined with insecticides has been proposed as an alternative strategy for insect pest management. This IPM approach may offer effective ways to control pests, in addition to lowering the risk of chemical residues in the environment. Spodoptera litura (Fabricius) is a major pest of many crops like cotton, maize, tobacco, cauliflower, cabbage, and fodder crops globally. Here, we evaluated the combined effects of new chemistry insecticides (chlorantraniliprole and emamectin benzoate) and entomopathogenic bacterial strains, Shewanella sp. (SS4), Thauera sp. (M9) and Pseudomonas sp. (EN4) against S. litura larvae inducing additive and synergistic interactions under laboratory conditions. Both insecticides produced higher larval mortality when applied in combination with bacterial isolates having maximum mortality of 98 and 96% with LC50 of chlorantraniliprole and emamectin benzoate in combination with LC50 of Pseudomonas sp. (EN4) respectively. The lower concentration (LC20) of both insecticides also induced synergism when combined with the above bacterial isolates providing a valuable approach for the management of insect pests. The genotoxic effect of both the insecticides was also evaluated by conducting comet assays. The insecticide treatments induced significant DNA damage in larval hemocytes that further increased in combination treatments. Our results indicated that combined treatments could be a successful approach for managing S. litura while reducing the inappropriate overuse of insecticides.
Subject(s)
Insecticides , Humans , Animals , Spodoptera , ThaueraABSTRACT
The release of untreated/partially treated effluent and solid waste from textile dyeing industries, having un-reacted dyes, their hydrolysed products and high total dissolved solids (TDS) over the period of time had led to the deterioration of ecological niches. In an endeavour to develop a sustainable and effective alternative to conventional approaches, a plug flow reactor (PFR) having immobilized cells of consortium of three indigenous bacterial isolates was developed. The reactor was fed with effluent collected from the equalization tank of a textile processing unit located near city of Amritsar, Punjab (India). The PFR over a period of 3 months achieved 97.98 %, 82.22 %, 87.36%, 77.71% and 68.75% lowering of colour, chemical oxygen demand (COD), biological oxygen demand (BOD), total dissolved solids (TDS) and total suspended solids (TSS) respectively. The comparison of the phytotoxicity and genotoxicity of untreated and PFR-treated output samples using plant and animal models indicated significant lowering of respective toxicity potential. This is a first report, as per best of our knowledge, regarding direct treatment of textile industry effluent without any pre-treatment and with minimal nutritional inputs, which can be easily integrated into already existing treatment plant. The successful implementation of this system will lower the cost of coagulants/flocculants and also lowering the sludge generation.
