ABSTRACT
The authors studied the percentage of T-lymphocytes detected by means of acid non specific esterase technique in patients with malignant thyroid tumours. The lymphocytes were investigated in the punctates from the tumour as well as in the venous blood. The results were compared with those in simple goitres and in healthy controls respectively. It has been found that the percentage of T-lymphocytes in the tumours limited to the thyroid was significantly higher in comparison with those of disseminated tumours. In the venous blood of cancers with metastases the ANAE positive lymphocytes were decreased, when compared with thyroid cancers without spread as well as with healthy controls. After the operation the T-lymphocytes decreased in the blood to the values found in healthy people. The authors suppose that ANAE positive lymphocytes (corresponding to the T-lymphocytes) which play an important part in the anti-tumour cell defence might be used as a convenient criterion by which this aspect of immune state could be judged. Further experience will prove whether the presence of metastases could thus be assayed.
Subject(s)
T-Lymphocytes , Thyroid Neoplasms/immunology , Carboxylesterase , Carboxylic Ester Hydrolases , Goiter/immunology , Humans , T-Lymphocytes/enzymologyABSTRACT
In view of the discrepancy in the results concerning the question of inheritance of immunological tolerance, we tried to enhance the genetic effect of tolerization by tolerizing male mice over three succeeding generations and by testing the ability of their progeny to reject an allogeneic tumour graft. This highly sensitive in vivo test, even of partial tolerance, did not reveal any difference in allogeneic tumour growth between progeny of control and tolerized males. Thus, the results again did not confirm genetic transmission of induced tolerance. The possible causes of the discrepancy between the results reported here and the results obtained by others are considered.
Subject(s)
Immune Tolerance , Immunogenetics , Animals , Female , Graft Rejection , Male , Mice , Mice, Inbred Strains , Neoplasm Transplantation , Transplantation, HomologousABSTRACT
Mice of the inbred strains B10.A (H-2a), B10 (H-2b), and BALB/c (H-2d) were immunized with the syngeneic cells, and the sera obtained from individual animals on day 10 after the 8th immunizing injection were assayed for the presence of alloreactive cytotoxic antibodies. Anti-H-2Kk antibodies were present in the sera from 50% of the syngeneically immunized BALB/c mice. The syngeneic-immune B10 sera contained weak anti-H-2Dd antibodies in 14% of the immunized animals. B10.A mice produced no antibodies after syngeneic immunization.
Subject(s)
Antibodies/immunology , Cytotoxicity, Immunologic , H-2 Antigens/immunology , Mice, Inbred Strains/immunology , Animals , Immunization , Mice , Mice, Inbred BALB C , Mice, Inbred C57BLSubject(s)
Macrophages/immunology , Receptors, Mitogen , T-Lymphocytes/immunology , Animals , Cell Membrane/immunology , Female , In Vitro Techniques , Mice , Mice, Inbred A , Movement , Rats , Rats, Inbred StrainsABSTRACT
The tube leukocyte adherence inhibition (LAI) assay was used to follow the specific antitumor immunity in patients with tumors of the urogenital tract, carcinomas of the larynx, melanomas and lung tumors. Positive reactivity was observed in more than 80% of the patients with tumor, whereas less than 5% of the controls were positive. The results show that the tube LAI assay holds promise for diagnosing and monitoring cancer diseases.
Subject(s)
Immunologic Techniques , Leukocyte Adherence Inhibition Test , Neoplasms/diagnosis , Female , Humans , Laryngeal Neoplasms/diagnosis , Lung Neoplasms/diagnosis , Neoplasms/immunology , Urogenital Neoplasms/diagnosisABSTRACT
In agreement with previous results, we failed to observe hereditary transmission of transplantation tolerance in the progeny of tolerant male mice when tested by skin grafting. At the same time we showed that the detection of the tolerance state depended on the test of immunity used. The possibility has not excluded that positive results with hereditary transmission of tolerance via fathers [1-3] may be due to the appropriate detection systems.
Subject(s)
Immune Tolerance , Mice, Inbred C57BL/genetics , Spleen/transplantation , Animals , Cytotoxicity, Immunologic , Female , Graft Rejection , Lymphocyte Culture Test, Mixed , Male , Mice , Skin TransplantationSubject(s)
Immune Tolerance , Immunogenetics , Animals , Cytotoxicity Tests, Immunologic , Male , Mice , Mice, Inbred C57BL , Mice, Inbred StrainsABSTRACT
The tube LAI assay was used for long-term follow-up of specific antitumor immunity in larynx cancer patients. A high percentage of patients examined before surgery exhibited positive LAI reactivity, and the success of treatment was reflected in the disappearance of positive LAI reactions. The tube LAI assay may be useful in diagnosing the precancerous states. Finally, positive LAI reactions were observed in hospital staff members exposed to contact with cancer patients over several years.
