The effect of respiratory diseases on serum lactate dehydrogenase and its isoenzyme patterns in calves.

In this study we examined the serum activity of lactate dehydrogenase (LDH) and its isoenzyme patterns in 28 calves of a lowland black spotted breed and its crossbreeds at the age of 2-6 months suffering from clinically noticeable manifested respiratory diseases--bronchopneumonia (BRD Group). As a control group we used 35 clinically healthy calves of the same age, breed and nutrition (Healthy Group). The sick calves did not show clinical signs or pathological lesions on other organ systems. The results found in sick calves showed a significantly higher total activity of LDH than in clinically healthy animals (P < 0.01). The mean activity of LDH was 2012 U/I in healthy calves and in calves with respiratory diseases 2529 U/1. The differences in all LDH isoenzyme patterns between both groups of animals were significant (P < 0.001) and in calves with respiratory diseases are characterized by a marked increase of the LDH 1 fraction and a decrease in the proportion of the other four LDH isoenzymes. Our results differ from those observed and presented in respiratory diseases in human medicine or in sheep. The explanation for the obtained results in calves and the determination of their diagnostic significance needs further studies and investigations using more animals with various severity of clinical signs and pathological changes, including analysis and determination of lactate dehydrogenase isoenzyme patterns in healthy and affected cattle lung tissue.

The serum protein electrophoretic pattern and acute phase protein concentrations in calves with chronic respiratory diseases.

The aim of this study was to evaluate the serum protein electrophoretic pattern and the concentrations of acute phase proteins (haptoglobin, serum amyloid A, and fibrinogen) in 28 calves with clinical signs of chronic respiratory diseases and 36 healthy calves as a control group. In sick calves we found significantly higher serum concentrations of total proteins (P < 0.001), lower concentrations of albumin (P < 0.001) and marked shift in the concentrations of the most of protein fractions with significantly higher values of alpha1-, beta1-, beta2-, and gamma-globulins (P < 0.001 and P < 0.01). The affected calves had significantly higher values of haptoglobin, serum amyloid A, and fibrinogen as well (P < 0.05, P < 0.001 and P < 0.001, respectively).

The effect of supplementation of clove and agrimony or clove and lemon balm on growth performance, antioxidant status and selected indices of lipid profile of broiler chickens.

The study investigated the effects of diet supplementation with 1% clove flower buds powder combined with either 0.2% lemon balm extract or 0.2% agrimony extract (each of the two pulverized extracts supplied through drinking water) on body weight of broilers, total feed intake, feed conversion ratio and the carcass yield, activity of superoxide dismutase (SOD, EC 1.15.1.1) and glutathione peroxidase (GSH-Px, EC 1.11.1.9) in blood, concentration of sulfhydryl (-SH) groups, malondialdehyde (MDA), vitamin A and E, low-density lipoproteins in the blood plasma, serum cholesterol, total lipids, triglycerides and high-density lipoproteins in broiler chickens at 42 days of age. On the day of hatching, 120 male and female broilers of Cobb 500 were randomly divided into three groups. The control group (1st group) of broilers received a basal diet (BD) without any feed and water additive. Both experimental groups of chicks were fed BD enriched with clove (Syzygium aromaticum L.) powder at a dose of 10 g/kg DM for 42 days. Moreover, either lemon balm (Mellisa officinalis L.) extract or agrimony (Agrimonia eupatoria L.) extract diluted with drinking water (2:1000) was given to broilers in the 2nd and 3rd group respectively. The results indicated that feeding the diets enriched with selected herbal supplements failed to affect the growth performance of broiler chickens at 42 days of age. In addition, this supplementation had no influence on the activities of SOD and GSH-Px, concentration of vitamin A and selected lipid metabolism indices. On the other hand, we observed beneficial effects on some indices of the antioxidant status (increased concentration of -SH groups and vitamin E, decreased concentration of MDA) in the blood of broilers in both experimental groups in comparison with the control group of chickens (p < 0.05). Furthermore, a slightly better antioxidant capacity was found in the blood of broilers supplied the combination of clove and lemon balm compared to clove and agrimony (vitamin E, 11.26 ± 0.73 vs. 9.73 ± 0.64 µmol/L, p < 0.05 respectively). It could be concluded that supplementation of the diet with clove flower buds powder combined with lemon balm extract or agrimony extract dissolved in drinking water has a potential to increase the antioxidant status but fails to influence either the growth performance or the selected lipid metabolism indices of broilers at the age of 42 days.

Fusarial toxins and their role in animal diseases.

Fusarial toxins are toxic metabolites produced mostly by toxigenic micromycetes of genus Fusarium. Dominant mycotoxins of this group include trichothecenes, moniliformin, zearalenone, and fumonisins. Recently, special attention has been paid to these toxins because of their harmful effects on both animals and humans. On the basis of the available literature, we review here the characteristics of major fusarial mycotoxins with an emphasis on their toxic effects on animals. The most important fusarial mycotoxins, their sources, and their pathology including clinical signs, necropsy findings, as well as changes in haematological, biochemical, and immunological indices, are addressed.

The effect of arterial blood sampling sites on blood gases and acid-base balance parameters in calves.

In 21 healthy calves, 1-6 months old, the interrelationship and comparability of acid-base balance variables (pH, HCO3-, BE) and blood gases (pCO2, pO2, and sat-O2) were evaluated in arterial blood collected from a larger, centrally localised (the a. axillaris) and a smaller peripheral artery (the a. auricularis caudalis). Sampling was done by direct puncture of the vessels without local anaesthesia. Except for blood pH, significant differences were observed in the average values of pCO2, pO2, HCO3-, sat-O2 (P < 0.001), and BE (P < 0.05). Analyses of blood from the a. axillaris showed higher pH, pO2, and sat-O2 values, and lower pCO2, HCO3-, and BE values compared with that from the a. auricularis caudalis. Despite statistically significant differences between some variables, in all indices high and significant correlation relationships were recorded (R = 0.928-0.961; P < 0.001). Therefore, from the biological and clinical point of view, these differences are unimportant and the presented method of peripheral arterial blood sampling can be considered suitable for evaluating blood gases and acid-base status.

The effect of zearalenone on some enzymatic parameters in rabbits.

The effects of low (10 microg/kg b.w.) and high (100 microg/kg b.w.) doses of mycotoxin-zearalenone on selected blood serum enzyme activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), and total lactatedehydrogenase (LD) of rabbits were studied. The toxin was administered orally for 14 days. In the experimental group E(1) (10 microg/kg b.w.), a significant increase in ALP activity was observed at 168 and 336 h during the experiment. In the 100 microg zearalenone sera (group E(2)), significant increases in activities of AST, ALT, ALP, GGT, and LD were observed at 168 and 336 h, indicating possible liver toxicity due to chronic effects of the toxin.

The urinary excretion of selenium in sheep treated with a vasopressin analogue.

The renal excretion of selenium was investigated in ewes with an excretion of hypotonic urine (control group) and in ewes with a formation of highly concentrated urine. Chronic stimulation of the urinary concentrating activity of sheep kidneys was induced by a long-term treatment with 1-desamino-8-D-arginine vasopressin (dDAVP), a synthetic analogue of vasopressin with prolonged effects. Young animals with 22 to 25 kg b.w. were fed a normal protein diet providing a daily intake of 129.25 g of crude protein, 12.03 MJ of digestible energy and 0.18 mg of selenium for 3 weeks. The vasopressin treated sheep (n = 11) were given subcutaneous injections of 12.5 micrograms of dDAVP in glycerol twice daily for one week before the clearance measurement of renal functions. The control group (n = 11) was treated with glycerol only. The administration of dDAVP resulted in a highly significant decrease of the urinary flow rate (from 3.19 +/- 0.50 in control group to 0.33 +/- 0.03 mL.min-1 in dDAVP animals, P < 0.001) without changes in the glomerular filtration rate (80.18 +/- 6.36 in controls vs. 77.86 +/- 6.26 mL.min-1, NS). No effects on plasma selenium level were observed (0.17 +/- 0.03 in controls vs. 0.20 +/- 0.03 mumol.L-1, NS) but the amounts of selenium excreted were found to be highly significantly reduced (from 0.29 +/- 0.05 in controls to 0.03 +/- 0.01 nmol.min-1, P < 0.001) in dDAVP treated sheep. Despite a large reduction in urinary flow rate, the selenium concentration in urine was actually the same in both groups (0.09 +/- 0.01 mumol.L-1) resulting in a sharp fall in the renal clearance of selenium (2.20 +/- 0.54 in controls vs. 0.18 +/- 0.03 mL.min-1, P < 0.01) due to dDAVP. This seems to be a consequence of the large increase in the selenium solvent drag induced by a vasopressin treatment. The results presented suggest that vasopressin may contribute to maintenance of the selenium balance in sheep via its effects on renal function.

Study of immune response in pigs fed with high vitamin E-dosages.

The effects of long-term vitamin E-supplementation on phagocytic cells, lymphocyte sub-populations, and SWC3+ cell count were studied in pigs. Eighteen weaned pigs were divided into three groups: 1) 100 mg DL-alpha tocopheryl acetate/kg diet, 2) 200 mg DL-alpha tocopheryl acetate/kg diet, 3) control group (basic feed with 10 mg DL-alpha tocopheryl acetate/kg diet). The examination of the immune indices was performed on day 120 of feeding the supplemented diets. The higher dietary levels of vitamin E resulted in increased serum concentration of alpha-tocopherol for both experimental groups (p < 0.05) and there were no significant differences in counts of CD2+, CD4+, CD8+, B lymphocytes nor SWC3+ cells among the groups. Similarly, vitamin E supplementation did not affect the functions of phagocytic cells tested.

Influence of intoxication with organophosphates on rumen bacteria and rumen protozoa and protective effect of clinoptilolite-rich zeolite on bacterial and protozoan concentration in rumen.

The effect of O-ethyl-S-(2-diisopropylaminoethyl) methylthiophosphonate on rumen bacteria and rumen protozoa was investigated in sheep (after premedication with clinoptilolite-rich zeolite and without that premedication). In control animals a decrease in the total concentration of rumen protozoa was observed 3-7 d after intoxication (particularly in small and large ones). In clinoptilolite-rich-zeolite-treated animals only a slight decrease in protozoan numbers occurred during the first hours after the intoxication. Similarly, in every category of rumen bacteria marked differences between the groups were recorded, particularly in concentration of lipolytic bacteria. The results suggest some protective effect of clinoptilolite-rich zeolite for rumen microbiota against the organophosphate poison.

Plasma and liver alpha-tocopherol in dairy cows with left abomasal displacement and fatty liver.

Thirty dairy cows with left abomasal displacement (LAD) and 14 healthy control cows were studied to assess the status of the natural antioxidant vitamin E, lipid peroxidation in the liver (malondialdehyde-formation) and its relationship to hepatic lipidosis. Blood concentrations of alpha-tocopherol, aminotransferase, glutamin dehydrogenase, free fatty acids, beta-hydroxybutyrate and glucose were determined. alpha-tocopherol, tryglyceride, glycogen and malondialdehyde (MDA) in wer liver tissue samples were examined. The dietary alpha-tocopherol intake and its plasma changes in LDA cows were also investigated. Cows were divided into four groups according to their liver triglyceride contents (< 20; 20-80; > 80 mg/g of fresh tissue; and control groups). The lowest plasma vitamin E and the highest liver vitamin E levels were found in cows with highest hepatic triglyceride content. The highest increase in plasma alpha-tocopherol within 5 days was seen in cows with the lowest liver triglyceride (P < 0.01). The liver triglyceride was positively correlated with liver MDA (r = 0.38; P < 0.05) and negatively with plasma alpha-tocopherol (r = -0.41; P < 0.01).

Acute oral toxicity of the herbicide BUREX EKO in pheasants.

The aim of this study was to determine the acute LD50, clinical symptoms and pathological changes of acute BUREX EKO intoxication in pheasants according to OECD No 205. Medium lethal dose (LD50) of BUREX EKO in pheasant is 3.84 ml/kg body weight with the upper level of reliability 4.50 ml and lower level of reliability 3.27 ml/kg body weight. As far as the calculation to the effective substance is concerned it is 1077 mg of chloridazone per kg body weight with the interval of reliability from 919 to 1263 mg/kg body weight. Calculated the effective substance of chloridazone (3.84 ml is LD50 of BUREX EKO which contains 1077 mg of chloridazone) BUREX EKO can be classified as the moderately toxic substance to pheasants. There were following clinical symptoms of the BUREX EKO intoxication in pheasants: apathy, drowsiness, incapability to move, ruffled feathers, slight diarrhoea, strenuous respiration, tonico-clonical cramps before death, decease with the head expressively bent rearwards. There was a relatively fast beginning of rigor mortis in dead pheasants. Pathologico-anatomical dissection of the pheasants obtained under conditions of acute intoxication did not reveal any changes on the organs of both experimental and control pheasants which would be immediately connected with the effect of the administered substance. Hyperaemia was recorded by histologico-pathological investigation of the liver and kidneys. No changes on the brain and intestine wall were recorded.

Effects of iron and vitamin E administration on some immunological parameters in pigs.

The effects of intramuscular administration of iron and vitamin E on phagocytic functions of leucocytes and on T-lymphocyte percentages were studied. Twelve pigs with an average body weight (BW) of 40 kg were divided into four treatment groups: +E (20 mg tocopheryl acetate per kg BW); +Fe (10 mg iron dextran per kg BW); +E/+Fe (20 mg tocopheryl acetate + 10 mg iron dextran per kg BW), C (control). Blood samples were collected from the sinus ophthalmicus prior to administration the preparations, and then at 12, 24, 48, 72 hours, and 7 days after administration. The iron injection led to a significant increase (p < 0,05) in plasma iron concentrations in both groups given Ferridextran. An evaluation of the immunological parameters showed the greatest rise in T-lymphocyte percentages as well as in metabolic activity of phagocytes (tetrazolium reduction) in vitamin E treated groups (+E, +E/+Fe) within 24 hours after injection. No interactive effects between vitamin E and iron on the immunological parameters studied were found.

[The effect of vitamin E on cortisol and lactate levels and on the acid-base equilibrium in calves exposed to transportation stress]. / Vplyv vitamínu E na hladinu kortizolu, laktátu a acidobázickú rovnováhu u teliat vystavených transportnej zátazi.

The objective of this study was to examine the effect of vitamin E administration on plasma levels of cortisol and lactate, and on acid base balance in transported calves. In the study, eight calves, aged approx. ten days, divided into two groups, were used. 20 mg of tocopherol-acetate per kg body weight were administered orally to each of the four experimental calves 24 hours before loading. The calves were transported by road for 3 hours. Blood samples collected before and after the transportation were examined for acid base balance, lactate, and plasma vitamin E and cortisol levels. The administration of vitamin E led to a decrease of cortisol levels in 24 hours (from 7.6 +/- 9.5 to 4.2 +/- 0.2 nmol/l) as well as to a significant increase (p < 0.05) of plasma vitamin E levels 26 h after administration (from 2.52 +/- 1.36 to 12.28 +/- 6.14 mumol/l). There was no difference between the groups in cortisol response due to transportation stress (Tab. III). The transportation caused typical stress changes in lactate levels and acid base balance (lactacidaemia and the tendency to acidosis, Tab. III, IV). There was approx. threefold increase in plasma lactate concentrations due to transportation (from 2.49 +/- 0.69 to 6.35 +/- 3.75 mmol/l). The results of the present study demonstrated metabolic changes which has been reported to be typical of mild physiological stress reaction. In the present study, vitamin E had no significant effect on plasma levels of cortisol, and lactate, and acid-base balance.

[Biochemical aspects of the toxic effects of Supermethrin in a 140-day avian reproductive test]. / Biochemické aspekty toxického úcinku supermetrínu v podmienkach chronického 140-dnového aviárneho reprodukcného testu.

Chronic toxic effects of supermethrin on some biochemical parameters (AST, ALT, LDH, creatinine and total proteins) were investigated in 84 individuals of Japanese quail divided into four groups (control-K, experimental group I-P1, experimental group II-P2, experimental group III-P3) in the conditions of 140-day avian reproductive test. The three experimental groups received the tested substance at these doses: P1-10.7 mg/kg l.w./day, P2-21.4 mg/kg l.w./day, P3-35.7 mg/kg l.w./day. The results of observation of the enzyme activities AST and ALT show that only the AST activity (in the course of 140-day avian reproductive test) significantly increased to 1.225 mu kat/l in the females of experimental group P1, to 1.053 mu kat/l in P2 and to 1.014 mu kat/l in P3 against the control, in which the AST activity was 0.670 mu kat/l. The values of AST activity in the males were 1.143 mu kat/l in P1, 1.117 mu kat/l in P2 and 1.090 mu kat/l in P3 against the control 0.8395 mu kat/l. The investigation of variations in total LDH activity in Japanese quail after 140-day avian reproductive test has shown an increase in the LDH activity in the males (11.193 mu kat/l in P1, 11.269 mu kat/l in P2, 8.245 mu kat/l in P3 and 7.362 mu kat/l in K) as well as in the females (10.91 mu kat/l in P1, 12.023 mu kat/l in P2, 10.196 mu kat/l in P3 and 7.055 mu kat/l in K).(ABSTRACT TRUNCATED AT 250 WORDS)

Tissue and erythrocyte cholinesterase inhibition and protection by clinoptilolite pretreatment.

The inhibitory effect of VX, an organophosphate nerve agent, on tissue and erythrocyte cholinesterase (ChE) and the protective effect of clinoptilolite (Cli) in male Wistar rats were studied. The tissue and erythrocyte ChE activity were significantly inhibited 30 min after 65.5 micrograms VX/kg body weight administration. The greatest enzyme inhibition was observed in erythrocytes, stomach, brain and liver. The ChE activity after pretreatment with 1.0 g Cli/kg was significantly higher than that in the VX without Cli group in all samples except duodenum and colon.

[Effects of a surgical reposition of left-sided abomasal displacement on parameters of energy metabolism in dairy cows]. / Auswirkungen einer operativen Reposition der linksseitigen Labmagenverlagerung auf Parameter des Energiestoffwechsels bei Milchkühen.

The objective of this study was to investigate whether the surgical replacement of left abomasal displacement (omentopexy) leads to typical stress changes in the energetic metabolism of dairy cows: twenty dairy cows, aged approximately 4.4 years were used. Six blood samples were collected in order to determine concentrations of cortisol, glucose, free fatty acids, L(+)-lactate, and beta-hydroxybutyrate once before, and five times after surgery. The surgical intervention led to a significant elevation in cortisol, glucose, FFA and lactate blood levels. These stress-related changes were observed over a 24 hour-period after surgery. In contrast to these parameters, serum concentration of beta-hydroxybutyrate slowly decreased over the entire observation period (72 hours). The results show that surgical correction of left abomasal displacement has a short-term stress effect on energy metabolism of dairy cows.

Effect of vitamin E on some leucocytic parameters and functions in transported calves.

The objective of this investigation was to determine the effect of vitamin E administration on leucocyte parameters and some of their functions in transported calves. In the study 8 calves, aged approx. 10 days, divided into two groups, were used. 20 mg of tocopheryl-acetate per kg body weight were administered orally to each of the 4 experimental calves 24 hours before loading. The calves were transported by road for 3 hours. Blood samples collected before and after the transportation were examined for total and differential leucocyte counts, T-lymphocyte subpopulation, phagocytic activity, leucocyte migration, serum immunoglobulin levels, and for plasma vitamin E and cortisol levels. The animals showed a leucocytosis with neutrophilia and lymphopenia after transportation. The administration of vitamin E led to a decrease of cortisol level in 24 hours. There was no difference between groups in cortisol reaction due to transportation stress. Leucocyte migration has been less inhibited in the control group after unloading. A mild decline in phagocytic activity was observed 3 hours after transportation. Serum immunoglobulins were unaffected by both vitamin E administration and transportation. Vitamin E had no significant effect on leucocyte function and cortisol levels in present study.

Effect of vitamin E injection on cortisol and white blood cell response to surgical stress in dairy cows.

In 20 dairy cows the effect of a single vitamin E injection on cortisol and white blood cell response to surgical stress was investigated. Abdominal surgery (omentopexy) was carried out in order to correct a left abomasal displacement. Ten hours prior to surgery 10 cows were injected intramuscularly with 10 mg DL-alpha-tocopheryl acetate per kg body weight. Ten cows (controls) received an equivalent volume of injectable water. Over a 72 hour period plasma samples were collected. In 8 cows (4 cows from each group) alpha-tocopherol in liver tissue was determined. Plasma vitamin E concentrations were significantly higher in vitamin E injected cows than in controls in all of the samples after the vitamin E injections. Similarly, liver alpha-tocopherol concentrations were significantly higher in vitamin E injected cows than in controls. The surgical stress led to profoundly increased plasma cortisol levels and leucocytosis due to neutrophilia within 5 hours after the surgery. Plasma cortisol levels as well as leucocyte counts were not affected by route of vitamin E administration used.

[New findings on serum lactate dehydrogenase isoenzymes in pigs during slaughter]. / Nové poznatky o izoenzýmoch laktátdehydrogenázy v krvných sérach osípaných pri porázke.

In the article we describe lactate dehydrogenase-(LD) (EC 1.1.1.27) isoenzyme pattern detected in the sera of pigs at slaughter. The pattern was different from that of normal serum (Fig. 1) and was characterized by the occurrence of an extra LD-fraction in the cathodic site of LD4 (Fig. 2). This fraction was unusual due to its unwillingness to separate by native polyacrylamide gel electrophoresis (PAGE) and took shape of a diffuse zone. The presence of the extra LD-zone caused a proportional decrease in quantitative distribution of the other LD forms, especially LD1 to LD3, in slaughtered pig sera (Tab. I). We examined the homogeneity of an apparent LD5-fraction using gel isoelectric focusing (IEF). We found out that after separation in a gradient of pH (3-9) two to three new extra bands with LD activity appeared in the area with relatively high pH value (pH 9) (Fig. 3). Their localization in the gradient of pH was greatly different from that of true LD molecules, the latter being situated in more acidic area. It is obvious from the finding described above that the diffuse LD-zone, detected in the serum of pigs at slaughter by native PAGE, was in no case a homogeneous protein. Consequently, it eliminates a possibility that the extra LD fraction reflects an increased LD5 activity in serum of affected animals. On the contrary, the IEF showed that the diffuse LD-zone consisted of two to three electrophoretically distinct proteins with relatively high pI values. As these proteins differed in their electrophoretic properties from the true LD isoenzymes we denoted them LD-like proteins. An origin of the unusual LD-like proteins detected in the serum of pigs at slaughter remains unknown for us for the time being.