Microplastic pollution in small rivers along rural-urban gradients: Variations across catchments and between water column and sediments.

The aquatic ecosystems of the world are highly burdened with microplastics (MPs; particles <5 mm). There is a great need for better understanding of patterns of MP pollution across catchments and rivers of different sizes, anthropogenic pressures and hydrogeomorphological features. In this study, we investigated the MP concentrations including their characteristics (polymer type, shape, size and colour), and MP distribution in water and sediments of two hydrogeomorphologically different small-scale catchments (< 800 km2), namely Kamniska Bistrica (KB) and Ljubljanica (LJ), Slovenia. The main objective of this study was to gain a better understanding of how WWTP effluents and catchment urbanisation together with the diversity of natural hydrogeomorphology, affect the quantity and quality of MP pollutants in the rivers with smaller catchments. Significantly different mean MP concentrations were found in the water columns (KB: 59 ± 16 items m-3; LJ: 31 ± 14 items m-3), but not in the sediments (KB: 22 ± 20 items kg-1; LJ: 23 ± 25 items kg-1). A longitudinal gradient with increasing particle concentration was observed in both water and sediment samples and in both catchments. Polyethylene (PE) and polypropylene (PP) particles dominated in all samples. Fibres were predominant in the water column samples, while fragments were more common in the sediment samples. MP particles were mostly coloured, and most of them were smaller than 2 mm in both water and sediment samples. The critical evaluation of the results and previous studies suggest that the characteristics of the catchment (anthropogenic pressures, size, climate, etc.), the hydrogeomorphology of the river (sediment type, discharge, flow velocity etc.), the sampling location along the river, the sampled compartment (water, sediment), the sampling method, and the hydrometeorological characteristics at the time of sampling, are important factors for observed MP concentrations and other characteristics.

Marine litter in the Croatian part of the middle Adriatic Sea: Simultaneous assessment of floating and seabed macro and micro litter abundance and composition.

In this study, abundance, distribution and composition of floating and seabed macro and micro litter in the Central Adriatic Sea were assessed. Floating macro litter observations were made. Floating and seabed micro litter were sampled with manta net and Van Veen grab, respectively. Micro litter particles visually found under the microscope were chemically analyzed with Fourier Transform Infrared microscope. Average calculated concentrations of floating macro (175 items/km2), floating micro (127 thousand particles/km2) and seabed micro litter (36 particles/100 g dry weight) show similar values as other published studies from the Mediterranean Sea. A statistically significant (p < 0.01) correlation between the floating micro and macro litter concentrations was found for the sites located in the channel waters. Disagreement between model and observations revealed gaps in our knowledge concerning the sea circulation and litter sources. Simultaneous samplings and observations of marine litter in different marine compartments proved possible, efficient and informative.

Assessment on marine litter ingested by fish in the Adriatic and NE Ionian Sea macro-region (Mediterranean).

This study presents data on the marine litter occurrence in the stomachs of fish species living in different marine habitats for the Adriatic and NE Ionian Sea macro-region. "Macro-litter" was examined in 614 specimens belonging to 11 species, while micro-litter in 230 specimens belonging to 7 species. The study highlights for the first time the presence of litter in the stomachs of the fish species Citharus linguatula. The occurrence of "macro-litter" in the guts of fish was <3% in both the NE Ionian and N Adriatic but reached 26% in the S Adriatic Sea. Micro-litter occurrence was 40 for the NE Ionian and increased to 87% in the N Adriatic (Slovenian Sea). The ingested "macro" and micro-litter differed among the areas. The marine habitat was found to affect the "macro"-litter ingestion but not the micro-litter.

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis.

Plastic resin pellets, categorized as microplastics (≤5 mm in diameter), are small granules that can be unintentionally released to the environment during manufacturing and transport. Because of their environmental persistence, they are widely distributed in the oceans and on beaches all over the world. They can act as a vector of potentially toxic organic compounds (e.g., polychlorinated biphenyls) and might consequently negatively affect marine organisms. Their possible impacts along the food chain are not yet well understood. In order to assess the hazards associated with the occurrence of plastic pellets in the marine environment, it is necessary to develop methodologies that allow for rapid determination of associated organic contaminant levels. The present protocol describes the different steps required for sampling resin pellets, analyzing adsorbed organochlorine pesticides (OCPs) and identifying the plastic type. The focus is on the extraction of OCPs from plastic pellets by means of a pressurized fluid extractor (PFE) and on the polymer chemical analysis applying Fourier Transform-InfraRed (FT-IR) spectroscopy. The developed methodology focuses on 11 OCPs and related compounds, including dichlorodiphenyltrichloroethane (DDT) and its two main metabolites, lindane and two production isomers, as well as the two biologically active isomers of technical endosulfan. This protocol constitutes a simple and rapid alternative to existing methodology for evaluating the concentration of organic contaminants adsorbed on plastic pieces.

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis.

Microplastic pollution in the marine environment is a scientific topic that has received increasing attention over the last decade. The majority of scientific publications address microplastic pollution of the sea surface. The protocol below describes the methodology for sampling, sample preparation, separation and chemical identification of microplastic particles. A manta net fixed on an ¼A frame« attached to the side of the vessel was used for sampling. Microplastic particles caught in the cod end of the net were separated from samples by visual identification and use of stereomicroscopes. Particles were analyzed for their size using an image analysis program and for their chemical structure using ATR-FTIR and micro FTIR spectroscopy. The described protocol is in line with recommendations for microplastics monitoring published by the Marine Strategy Framework Directive (MSFD) Technical Subgroup on Marine Litter. This written protocol with video guide will support the work of researchers that deal with microplastics monitoring all over the world.

Mercury induced community tolerance in microbial biofilms is related to pollution gradients in a long-term polluted river.

The net toxicity of different forms of mercury, in the long-term during their transformation processes, leads to the selection of resistant bacterial cells and this result in community tolerance which is pollution induced. Accordingly, based on profiles of a bacterial community structure, analysis of Hg resistant culturable bacteria and quantification of merA genes, we assessed development of pollution induced community tolerance in a mercury-polluted gradient in the Idrijca River. TTGE analysis did not show effects of mercury pollution to bacterial community diversity, while quantification of merA genes showed that merA genes can be correlated precisely (R(2)=0.83) with the total concentration of mercury in the biofilm microbial communities in the pollution gradient.