ABSTRACT
The changes in antibiotic sensitivity of the purulent-necrotic processes agents under the low-intensive laser irradiation impact were studied in experimental part of the investigation. There were 156 patients, suffering neuropathically-infected form of the diabetic foot syndrome, examined and treated, in 41 of them the iodine deficiency was revealed. Durable regional intaarterial infusion, low-intensive laser irradiation and preparations of iodine in a daily dose of 200 mcg were applied. High efficacy of the antibacterial therapy photomodification was proved, it have permitted to improve the results of treatment, to refuse from disabling operations in majority of the patients, to secure the foot bearing function. While the iodine deficiency presence the results of treatment are improved due to application of the iodine preparations.
Subject(s)
Diabetic Foot/therapy , Iodine/therapeutic use , Low-Level Light Therapy/methods , Necrosis/therapy , Pseudomonas Infections/therapy , Staphylococcal Infections/therapy , Aged , Anti-Bacterial Agents/therapeutic use , Diabetic Foot/microbiology , Diabetic Foot/pathology , Diabetic Foot/surgery , Disease Management , Drug Resistance, Bacterial , Female , Humans , Iodine/deficiency , Male , Middle Aged , Necrosis/microbiology , Necrosis/pathology , Necrosis/surgery , Pseudomonas Infections/microbiology , Pseudomonas Infections/pathology , Pseudomonas Infections/surgery , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/growth & development , Staphylococcal Infections/microbiology , Staphylococcal Infections/pathology , Staphylococcal Infections/surgery , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & developmentABSTRACT
The cellular and lipopolysaccharide (LPS) fatty acid compositions of the type strains of Klebsiella pneumoniae, K. oxytoca, K. terrigena, K. planticola, and "K. trevisanii" were studied. The cellular fatty acids of klebsiellae were presented by straight-chain saturated and monounsaturated, cyclopropane, and hydroxy fatty acids. Hexadecanoic, methylenehexadecanoic, octadecenoic and hexadecenoic acids prevailed. The K. pneumoniae strain mainly differed from the strains of other species by two and more times lower level of dodecanoic acid in cells. Variations of cyclopropane and unsaturated fatty acid contents in cells were observed. LPS fatty acids profiles of klebsiellae mainly consisted of straight-chain saturated and hydroxy fatty acids with predominance of tetradecanoic and 3-hydroxytetradecanoic acids. LPS fatty acids profiles of K. oxytoca, K. terrigena, K. planticola, and "K. trevisanii" strains were very similar and differed from that of the K. pneumoniae strain by higher levels of dodecanoic acid (approximately 5-6 times) and absence of 2-hydroxytetradecanoic acid. The obtained data indicated more close relatedness of K. oxytoca, K. terrigena, and K. planticola and some their remoteness from K. pneumoniae.
Subject(s)
Fatty Acids/analysis , Klebsiella/chemistry , Lipopolysaccharides/chemistry , Chromatography, Gas , Klebsiella/classification , Klebsiella pneumoniae/chemistry , Klebsiella pneumoniae/classification , Lipopolysaccharides/isolation & purification , Phenotype , Species SpecificityABSTRACT
Bacteria of genus Klebsiella were found to possess group-specific antigens of ABO system. Representatives of conditionally pathogenic species, K. rhinoscleromatis, K. pneumoniae, K. ozaenae, K. oxytoca, contain 9.4 to 38% of the antigens. In the process of interaction of plant organisms and Klebsiella, which passed three times in green tomato fruits, the latter have been found to influence some biological features of the bacteria which is manifested in the loss of the mimicry antigens by a considerable part of the strains as well as in developing capsules by some capsule-free variants of klebsiellae.
