ABSTRACT
The work includes a research of morphological and immune-histochemical specifics of baby rats' cerebral structures in the conditions of experimental hypoxia model and with the administration of Cerebrocurin. It was ascertained that the most pronounced changes in the form of pericellular and perivascular edema, spongiform loci and loci of necrosis, with the phenomena of apoptosis and aponeuroses, occurred during severe hypoxia. Administration of Cerebrocurin in baby rats, which experienced moderate hypoxia, causes a decrease in pathological changes in cerebral structures and the approximation of the level of CD95 APO-1/Fas and Bcl-21 gene expression to their level in intact animals.
Subject(s)
Brain Stem/pathology , Hypoxia/pathology , Neurons/pathology , Neuroprotective Agents/therapeutic use , Animals , Animals, Newborn , Apoptosis/drug effects , Brain Diseases/immunology , Brain Diseases/metabolism , Brain Diseases/pathology , Brain Diseases/prevention & control , Brain Stem/drug effects , Brain Stem/immunology , Brain Stem/metabolism , Disease Models, Animal , Gene Expression/drug effects , Hypoxia/immunology , Hypoxia/metabolism , Neurons/immunology , Neurons/metabolism , Neuroprotective Agents/administration & dosage , Neuroprotective Agents/chemistry , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , fas Receptor/genetics , fas Receptor/metabolismABSTRACT
The article presents results of the study of peopleware of anesthesiology and intensive therapy service of medical institutions in Ukraine of II-III levels.
Subject(s)
Anesthesiology , Critical Care , Delivery of Health Care/standards , Medical Staff, Hospital/standards , Anesthesiology/standards , Anesthesiology/trends , Critical Care/standards , Critical Care/trends , Delivery of Health Care/trends , Medical Staff, Hospital/trends , Quality of Health Care , Ukraine , WorkforceABSTRACT
The authors have studied changes of activity of succinate dehydrogenase (SD) of peripheral blood lymphocytes of newborn with hypoxic-ischemic encephalopathy at early neonatal period and against cerebrokurin medicine administration. Newborn children with hypoxic-ischemic encephalopathy due to severe asphyxia have significant disturbancies of cyto-energy metabolism which is proved through decrease in activity index of SD and decrease in quantity of the cells with moderate and high activity. Lymphocytes of newborns with severe asphyxia were caracterized on six days by absence of the cells with moderate and high activity. Newborns with severe asphyxia who had been prescribed cerebrokurin had on their six days considerable increase in lymphocytes with moderate and high activity.
Subject(s)
Asphyxia Neonatorum/complications , Energy Metabolism , Hypoxia-Ischemia, Brain , Lymphocytes/metabolism , Amino Acids/administration & dosage , Amino Acids/therapeutic use , Asphyxia Neonatorum/blood , Drug Combinations , Energy Metabolism/drug effects , Gestational Age , Humans , Hypoxia-Ischemia, Brain/blood , Hypoxia-Ischemia, Brain/etiology , Hypoxia-Ischemia, Brain/metabolism , Hypoxia-Ischemia, Brain/prevention & control , Infant, Newborn , Lymphocytes/drug effects , Lymphocytes/enzymology , Peptides/administration & dosage , Peptides/therapeutic use , Succinate Dehydrogenase/blood , Succinate Dehydrogenase/metabolism , Treatment OutcomeABSTRACT
The authors carried out the sudy on morphofunctional changes of mitochondria of neurocytes of the brainstem of rats under experimental model of hypoxia and administration of Cerebrokurin and lipin. Cerebrokurin administration to rats which underwent severe chronic hypoxia lead to increase in mitochondria diameter and considerable increase of their area in the unit of volume as well it resulted in renewal of myelin sheaths. Use of Cerebrokurin and lipin in animals which underwent moderate hypoxia lead to probable increase in mitochondria area in the unit of volume in comparison with animals not treated with these agents.
