ABSTRACT
Thermal imaging and laser Doppler flowmetry were applied to study the effects of infrared (IR) radiation mediated by vascular response in healthy volunteers during regular unilateral arteriovenous occlusion of one of the fingers (4 times a day for 7 months with a gradual increase in exposure from 10 to 30 min). After repeated unilateral exposure, we observed consistent changes in temperature conditions and modes of thermoregulation of corresponding skin areas of both hands. We discovered a high level of positive correlation between the dynamics of IR emission from the experimental and control skin areas in the recovery phase after clamping, which indicates that systemic vascular response is involved. The possible mechanisms of post-occlusive changes in skin of the distal phalanges are discussed. These data suggest that multiple regular unilateral arteriovenous occlusions enhance the intradermal blood flow and can be used for the prevention of disorders of the peripheral microcirculation in various pathological conditions.
Subject(s)
Ischemia/physiopathology , Microcirculation/physiology , Skin/blood supply , Temperature , Wrist/blood supply , Adult , Fingers/blood supply , Fingers/physiopathology , Healthy Volunteers , Humans , Laser-Doppler Flowmetry , Male , Skin/physiopathology , Wrist/physiopathologyABSTRACT
The goal of this work was to describe methodological approaches to determination of sensitivity and specificity of the enzyme-linked immunosorbent assay kit (ELISA Kit) for detection of the specific anti-Junin virus (JV) antibody. Comparison of ELISA to plaque reduction neutralization test (PRNT) showed direct relationship between antibody titers in the samples of serum of immunized animals, determined by either PRNT or ELISA methods. The obtained results provided an opportunity to form the panels of positive and negative serum samples to determine the sensitivity and specificity of the ELISA Kit. Sensitivity of the ELISA Kit was at least 98% when studying the samples of serum of immunized guinea pigs and rabbits (determined as positive in PRNT). The sensitivity of the ELISA Kit was at least 68% when studying the samples determined by PNRT as uncertain positive. The specificity was 98%. The specificity of the ELISA Kit was 98%.
