ABSTRACT
The cytotoxic action of lectins different in origin and carboxyl specificity has been studied. It has been shown that all types of lectins at high concentrations (20 mkg/ml) were able to induce apoptosis in the in vitro populations of Chinese hamster cells two days after the treatment. In the case of Persa fluviatilis lectin this effect was detected immediately after the treatment and two days later as well. It was shown that Sambucus nigra lectin did not influence the frequency of apoptosis in the culture of human cells in contrast to the Lens culinaris and P. fluviatilis lectins. The tendency of stimulation of human cell proliferation under exposure to P. fluviatilis lectin at low concentration (0.2 microg/ml) has been registered.
Subject(s)
Apoptosis/drug effects , Lectins/pharmacology , Animals , Cell Line , Cell Nucleus/drug effects , Cell Nucleus/ultrastructure , Cell Proliferation/drug effects , Cricetinae , Cricetulus , Dose-Response Relationship, Drug , Humans , Plant Lectins/pharmacologyABSTRACT
Human albumin is able to induce statistically significant increasing of the frequency of spontaneous mutations in the hprt locus in cultured somatic mammalian cells. The strait dependence of the mutagenic effect on the protein concentration has been shown. The products of albumin degradation reveal antimutagenic activity in the investigated test-system.
Subject(s)
Albumins/pharmacology , Mutation/drug effects , Animals , Cells, Cultured , Cricetinae , Cricetulus , Dose-Response Relationship, Drug , Humans , Hypoxanthine Phosphoribosyltransferase/genetics , Mutagenicity TestsABSTRACT
The enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) has been revealed to be present in human cell cultures (fibroblasts and cardiomyocytes) and Chinese hamster culture cells. At the same time the enzyme AGT is not reproduced in the human brain shell culture cells.
