Subject(s)
Drosophila Proteins , Gene Duplication , Insect Proteins/genetics , Microsatellite Repeats/genetics , Amino Acid Sequence , Animals , DNA, Complementary , Drosophila melanogaster/genetics , Insect Proteins/chemistry , Molecular Sequence Data , Sequence Homology, Amino Acid , Transcription, GeneticABSTRACT
The effect of gonadotropins (PMSG/HCG) dose on number, quality and cytogenetic characteristics of CBA mouse oocytes was investigated. The oocytes from natural cycle were used as a control. Using of 5 IU dose led to increasing of oocytes number with no differences in their parameters from control ones. Percentage of oocytes of bed quality, with nuclear material degeneration, and parthenogenetically activated oocytes were found to increase under 10 IU and 15 IU dosage.
Subject(s)
Chorionic Gonadotropin/pharmacology , Gonadotropins, Equine/pharmacology , Oocytes/drug effects , Animals , Chromosome Aberrations/genetics , Dose-Response Relationship, Drug , Female , Metaphase/drug effects , Mice , Mice, Inbred CBA , Oocytes/cytology , Parthenogenesis/drug effects , Superovulation/drug effectsABSTRACT
A certain decrease in the content of riboflavin in blood plasma and liver and its excretion in the urine were encountered 24-h after administration of phthalazol, according to the schedule of acute dysentery treatment, in rats with initial moderate deficiency in vitamins B1 and B2. The provision with vitamin B1 did not change in this case. The introduction of phthalazol was attended with a noticeable decrease in the excretion of pyridoxic acid but did not affect the B6 content in the liver and blood serum of the animals.
Subject(s)
Anti-Infective Agents/pharmacology , Sulfathiazoles/pharmacology , Vitamin B Complex/metabolism , Animals , Anti-Infective Agents/administration & dosage , Liver/chemistry , Liver/drug effects , Liver/metabolism , Male , Rats , Spectrometry, Fluorescence , Sulfathiazoles/administration & dosage , Time Factors , Vitamin B Complex/analysisABSTRACT
It has been estimated that ten-day rectal administration of metazid in the form of suppositories (20 mg per 100 g of body mass) gives rise to vitamin B2 deficiency both in rats with initial vitamin B2 insufficiency and in those adequately supplied. This is confirmed by a decrease in the vitamin B2 content both in the liver and in the blood plasma. Development and use of suppositories containing combined preparation of metazid and riboflavin (in the dose of 100 micrograms per a rat according to the daily recommended allowance) completely prevent metazid-induced vitamin B2 deficiency.
Subject(s)
Antitubercular Agents/adverse effects , Isoniazid/analogs & derivatives , Riboflavin Deficiency/chemically induced , Riboflavin Deficiency/prevention & control , Animals , Antitubercular Agents/administration & dosage , Isoniazid/administration & dosage , Isoniazid/adverse effects , Rats , Riboflavin/metabolism , Riboflavin Deficiency/metabolism , SuppositoriesABSTRACT
Rectal administration of metaside (20 mg per 100 g body weight) as suppositories for 10 days was found to cause vitamin B2 deficiency both in rats on low-vitamin B2 diet and in those on balanced diet. This appeared as decreased vitamin B2 levels in the liver and plasma. The design and application of suppositories containing the mixed preparation of metaside and riboflavin drug (in a dose of 100 micrograms per rat, which corresponds to the recommended daily requirement) completely prevents vitamin B2 deficiency.
Subject(s)
Antitubercular Agents/pharmacology , Isoniazid/analogs & derivatives , Riboflavin Deficiency/chemically induced , Riboflavin Deficiency/prevention & control , Animals , Antitubercular Agents/administration & dosage , Drug Combinations , Drug Evaluation, Preclinical , Isoniazid/administration & dosage , Isoniazid/pharmacology , Liver/drug effects , Liver/metabolism , Rats , Riboflavin/administration & dosage , Riboflavin/metabolism , Riboflavin/pharmacology , Riboflavin Deficiency/metabolism , SuppositoriesABSTRACT
It was established that 10-day administration of suppository methazide (20 mg per 100 g b. w.) induces B2 vitamin deficiency indicated by relevant hepatic and plasmic values. In vitamin B2 deficiency methazide-induced changes in vitamin B6 metabolism are less marked in rats provided with riboflavin. Use of suppository methazide in combination with riboflavin (100 micrograms per animal which is a recommended daily dose) prevents B2 deficiency. It is recommended daily use combinations of methazide with riboflavin or piridoxin in essential daily consumption doses to treat patients with alimentary vitamin B2 and B6 deficiencies. This will not only prevent side effects of methazide, but also help to overcome deficiency of the above vitamins.
