1.
Biochemistry (Mosc)
; 69(3): 299-305, 2004 Mar.
Article
in English
| MEDLINE
| ID: mdl-15061697
ABSTRACT
Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
Subject(s)
Adenine/chemistry , Bacillus/enzymology , Cytosine/chemistry , DNA Modification Methylases/chemistry , DNA Modification Methylases/isolation & purification , DNA-Cytosine Methylases/chemistry , DNA-Cytosine Methylases/isolation & purification , Deoxyribonucleases, Type II Site-Specific/chemistry , Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry , Site-Specific DNA-Methyltransferase (Adenine-Specific)/isolation & purification , Chromatography, Liquid , DNA Methylation , Substrate Specificity
2.
Nucleic Acids Res
; 19(15): 4296, 1991 Aug 11.
Article
in English
| MEDLINE
| ID: mdl-1831259
