[Polyamines as biochemical markers of the antiproliferative effect of inhibitors of polyamine and putrescine biosynthesis enzymes in L-cell tissue cultures]. / Poliaminy kak biokhimicheskie markery antiproliferativnogo deistviia ingibitorov fermentov biosinteza poliaminov i putrestsina v kul'ture tkani L-kletok.

Effects of inhibitors of enzymes involved in biosynthesis of putrescine and polyamines on growth of mice C3H strain transformed fibroblasts as well as on the intracellular content of putrescine and polyamines were studied. Putrescine and polyamines were estimated as dansyl derivatives using HPLC. DL-alpha-difluoromethyl ornithine (F2MeOrn) and methyl glyoxalbis (quanyl hydrazone) (MGBG) were added to cultivation medium at final concentration 10(-4) M daily within 4 days. The cell culture was not treated within the following 3 days of the experiment. Distinct exceeding of spermidine content over concentrations of putrescine and spermine proved to be the specific property of L-cells growth in the culture. Amount of cells correlated strongly with intracellular content of spermidine and spermine (r = 0.964). Treatment of L-cells with F2MeOrn singly or in combination with MGBG led to 4- and 6-fold decrease in their growth rate, respectively. F2MeOrn altered content of polycations 2 days earlier in the cells which exhibited distinct correlation between putrescine and polyamines (r = 0.943). The rate of MGBG inhibition correlated distinctly with content of putrescine and polyamines (r = 0.953) as well as strong correlation between putrescine and polyamines content (r = 0.994) was detected after simultaneous effect of these inhibitors. This suggests that polyamines were involved in the antiproliferative action of the inhibitors studied and may be used as markers of cytostatics chemotherapeutic efficiency.

[Effect of erythropifadin on the morphofunctional characteristics of donor blood preserved for a long time]. / Vliianie éritropifadena na morfofunktsional'nye kharakteristiki dlitel'no khranimoi donorskoi krovi.

Adenine- and riboxine-containing erythropifaden has been studied for possibility to be used for restoring a functional value of erythrocytes in conserved donor blood stored for 35-49 days. Energy supply and oxygen transport function of erythrocytes are shown to get normal and their morphological composition--to get considerably improved. Besides, osmotic resistance and deformability of erythrocytes insignificantly change while concentration of riboxine in blood and that of substances with average molecular mass remain considerably high. One of the ways to remove the above shortcomings is application of hemosorbents.

[Study of middle molecular weight fraction in the plasma of patients with uremia using high performance liquid chromatography and thin layer chromatography]. / Issledovanie srednemolekuliarnoi fraktsii plazmy krovi bol'nykh s uremiei s pomoshch'iu zhidkostnoi khromatografii vysokogo davleniia i tonkosloinoi khromatografii.

Detection and quantitative estimation of peptides were carried out in the middle molecular fraction of blood plasma from patients with uremia using high performance liquid chromatography and thin-layer chromatography. The middle molecular fraction was separated into four fractions after ultrafiltration through a column of Protein Pak i-60. As low as 40-60 mcg/ml of the peptides were detected in the first and second fractions.