ABSTRACT
Stability and radical scavenging activity of betalains, biosynthesized by Beta vulgaris hairy root culture were investigated at different pH values, bile salts concentration, as well as at the in vitro conditions of gastrointestinal tract. It was established that pH below 3 and concentrations of the bile salts up to 4% had no great influence on betalains stability. At the in vitro conditions of gastrointestinal tract betalains are relatively stable, as their radical scavenging activity decrease from 75% inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH*) to about 38%.
Subject(s)
Beta vulgaris/chemistry , Free Radical Scavengers/metabolism , Gastrointestinal Contents/chemistry , Quaternary Ammonium Compounds/metabolism , Betalains , Cells, Cultured , Humans , Hydrogen-Ion Concentration , Models, Biological , Oxidation-Reduction , Plant Roots/chemistryABSTRACT
From a study of the relationship between the type and age of the inocula, and the growth and biosynthesis of betalains in a Beta vulgaris hairy root culture, the best results were achieved with a 14 d inoculum grown in submerged culture giving 42 mg betalains (16 mg betacyanins and 26 betaxanthins) and 1.5 g dry biomass in 40 ml medium.
Subject(s)
Beta vulgaris/growth & development , Beta vulgaris/metabolism , Plant Roots/growth & development , Plant Roots/metabolism , Quaternary Ammonium Compounds/metabolism , Aging/physiology , Beta vulgaris/physiology , Betalains , Culture Media/pharmacology , Culture Techniques/methods , Plant Extracts/analysis , Plant Roots/physiology , Quality Control , Quaternary Ammonium Compounds/classification , Quaternary Ammonium Compounds/isolation & purificationABSTRACT
Hairy root cultures were obtained from diploid and induced tetraploid plants of Datura stramonium and analyzed by gas chromatography/mass spectrometry. Twenty alkaloids (19 for diploid and 9 for tetraploid hairy root cultures) were identified. A new tropane ester 3-tigloyloxy-6-propionyloxy-7-hydroxytropane was identified on the basis of mass spectral data. Hyoscyamine was the main alkaloid in both diploid and tetraploid cultures. In contrast to diploid hairy roots, the percentage contributions of the alkaloids, with exceptions for hyoscyamine and apoatropine, were higher in the total alkaloid mixture of tetraploid hairy roots.
Subject(s)
Alkaloids/chemistry , Datura stramonium/chemistry , Plant Roots/chemistry , Alkaloids/isolation & purification , Cells, Cultured , Datura stramonium/genetics , Diploidy , Models, Molecular , Molecular Conformation , Plant Extracts/chemistry , Plant Extracts/isolation & purification , PolyploidyABSTRACT
Betalains biosynthesis and antiradical scavenging activity were investigated during cultivation of four hairy root cultures of Beta vulgaris, obtained from different cultivars (Bordo, Egyptian, Detroit 2 and Detroit Dark Red). The best producer of betalains was a hairy root culture from Beta vulgaris cv. Detroit Dark Red (13.27 mg/g dry weight total pigment production). The ethanol extract, derived from roots of the same culture grown for 15 days under submerged conditions, showed a high antiradical activity (83% of inhibition of the stable DPPH.).
